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81.
Eggs of Oryzius latipes in the blastula stage were exposed to M/100 artificial sea water which contained cadmium at the concentrations of 0.1, 1.0, 10.0, 20.0 or 50.0 mg 1−1 . The 96 h TL50 , value for cadmium was estimated to be 20 5 mg 1−1 . When the eggs were incubated for 24 h in the M/100 sea water with 10.0 mg Cd 1−1 and then rinsed in glycine buffer solution (pH; 2.0), the cadmium content of the egg decreased markedly. Cadmium levels were determined in parts of the embryonic body, the chorion and the yolk sac. The most cadmium was detected in the chorion (94.6%). Prolonged cadmium exposure revealed that most of the cadmium was absorbed by the chorion and little was detected in the embryonic body and the yolk sac. 相似文献
82.
Triton X-100 extract from brush border membranes of rat small intestine was recombined with egg phosphatidylcholine liposomes by the freeze-thaw sonication method. The treated liposomes showed a Na+-dependent uptake of cyclacillin, which was inhibited by a low concentration of mercuric ions and L-phenylalanylglycine, but not by glycine. These are consistent with the absorption characteristics of the antibiotic in situ and indicate that reconstitution of the Na+-dependent active cyclacillin transport system of rat small intestine has been achieved. 相似文献
83.
NG, a strain of cultured tobacco cells of Nicotiana glutinosahad high growth rates and carboxylate contents (CA) of100 to 130 meq/100 g of dry cells on media containing 42 meqNO3/liter as the sole N source. (CA) is the amount ofinorganic cations minus inorganic anions in meq per 100 g ofdry cells. NG, cultured on media containing NH4 10+NO3 42 in meq/liter,had lower growth rates and lower (CA) values as comparedwith NG on media containing NO3 as the sole N source. NG, cultured on media containing NH4 30+NO3 42 in meq/liter,had high growth rates and (AC) values of 22 to 53 meq/100gof dry cells. In this case, the (AC) content may correspondto organic cations, basic organic N compounds such as free asprotein-bound basic amino acids. The easily absorbed Cl mayhave been required maintain good growth conditions such as ionicbalance and a favorable pH in the cells. Thus cultured cells of Nicotiana glutinosa may have physiologicaladaptability against variations in a relatively wide range of|CA| contents [|CA| being the absolute valuesof (CA)]. (Received May 15, 1980; ) 相似文献
84.
Noriyuki Muranushi Noriko Takagi Shozo Muranishi Hitoshi Sezaki 《Chemistry and physics of lipids》1981,28(3):269-279
The effect of fatty acids and monoglycerides on barrier properties of liposomal membranes prepared from egg phosphatidylcholine was investigated. The incorporation of these lipids as liposomal membrane components induced the alteration of the permeability to less permeable liposomally entrapped drugs, sulfanilic acid and procainamide ethobromide (PAEB). Monoolein caused greatly increased permeability of both drugs and unsaturated fatty acids markedly enhanced the release rate of PAEB, while saturated fatty acids caused a small increase in the release rate.Electron spin resonance (ESR) investigation with 5-nitroxide stearic acid showed that fatty acids disordered the hydrophobic region of the lipid bilayer and the disordering effect of unsaturated fatty acids was greater than that of saturated ones. It was demonstrated that the incorporated fatty acids and monoglycerides interacted with the polar region of the membranes by ESR study with cholestane label and 1H-NMR study. These results indicated that the increase in the membrane permeability caused by fatty acids and monoglycerides associated with the disorder in the membranes' interior and the interaction of the incorporated lipid with the polar head group of phospholipid. 相似文献
85.
Hitoshi Iizumi Akihiko Hattori C.P. McRoy 《Journal of experimental marine biology and ecology》1980,47(2):191-201
The distribution of nitrate and nitrite in the interstitial water of the sediment of eelgrass (Zostera marina) bed of Izembek Lagoon, Alaska, were investigated. Their concentrations were relatively high (0 to 9.8 μg-at.N·1?1, average 4.8 for nitrate; 0 to 4.0 μ-at.N·1?1, average 1.9 for nitrite) although the sediments were anoxic and contained hydrogen sulphide. The rates of bacterial denitrification measured by 15N tracer technique ranged from 0.49×10?10 to 1.2 × 10?9 g-atN·g?1·h?1. When a steady state is maintained, the loss of nitrate and nitrite must be balanced by their production by bacterial nitrification. Experimentally determined rate of nitrification in the sediment was of the same order. A model experiment demonstrated that oxygen is transported from leaves to rhizomes and roots of eelgrass and released into the sediment. The oxygen is used for nitrification in the rhizosphere in anoxic sediments. 相似文献
86.
- When applying the adsorption theory to the selectivity coefficient,K and Mg uptakes by cells in a K-Mg replacement series of mediaare not regulated only by a common mechanism, under the assumptionthat b values, which indicate the affinities between the ionand the adsorptive surface, do not change.
- Regulation of Kand Mg uptakes by a common multiphasic mechanismin the cellsis possible when the selectivity coefficient bK/bMgvaries inverselywith the MK/MMg ratio in the external medium.
- K and Mg uptakesare not regulated only by their respectivesingle specific mechanisms.
- Another possibility is regulation of K and Mg uptakes bothbycommon and specific mechanisms. The common mechanism maybemultiphasic.
87.
Hitoshi Watanabe 《Journal of invertebrate pathology》1976,28(3):321-328
A microsporidian was isolated from the Egyptian cotton leafworm, Spodoptera litura, and was identified as a member of the genus Nosema since its sporont produced two spores. The size of the fresh spores was 3.3–4.5 × 1.7 – 2.2 μm. Mean length of the polar filaments was 90.7 μm. In addition to the Egyptian cotton leafworm, this microsporidian was also infectious to Pieris rapae crucivora, Hyphantria cunea, Chilo suppressalis, Agrotis ipsilon, and Adoxophyes orana. Although the parasite ultimately caused generalized infection in the Egyptian cotton leafworm larva, the main site of infection was the adipose tissue. 相似文献
88.
K Igarashi H Kumagai Y Watanabe N Toyoda S Hirose 《Biochemical and biophysical research communications》1975,67(3):1070-1077
The activities of ribonucleases (RNase HS and RNase A), which hydrolyze ribonucleic acid at linkages attached to pyrimidine nucleotides were stimulated by polyamines, while the activities of ribonucleases (RNase T1 and RNase M), which attack ribonucleic acid at linkages attached to purine nucleotides were not influenced by polyamines. In the presence of polyamines, the cleavage of C5′-O-P linkages adjacent to cytosine nucleotide was stimulated, while the cleavage of C5′-O-P linkages adjacent to uracil nucleotides was inhibited slightly. The effect of polyamines on the activities of ribonucleases occured through the binding of the polyamines to nucleic acid. 相似文献
89.
A PC survey revealed the occurrence of an unusual amino acid in fruit bodies of the mushroom Lactarius quietus Fr. and a related species. This was identified as l-2-amino-4-methylpimelic acid, which has not been previously reported as a naturally occurring compound. 相似文献
90.
Sleep and Biological Rhythms - 相似文献