全文获取类型
收费全文 | 1454篇 |
免费 | 89篇 |
国内免费 | 1篇 |
出版年
2022年 | 9篇 |
2021年 | 16篇 |
2020年 | 5篇 |
2019年 | 19篇 |
2018年 | 19篇 |
2017年 | 21篇 |
2016年 | 24篇 |
2015年 | 47篇 |
2014年 | 68篇 |
2013年 | 98篇 |
2012年 | 96篇 |
2011年 | 110篇 |
2010年 | 53篇 |
2009年 | 57篇 |
2008年 | 84篇 |
2007年 | 90篇 |
2006年 | 90篇 |
2005年 | 66篇 |
2004年 | 92篇 |
2003年 | 64篇 |
2002年 | 75篇 |
2001年 | 36篇 |
2000年 | 32篇 |
1999年 | 34篇 |
1998年 | 16篇 |
1997年 | 11篇 |
1996年 | 13篇 |
1995年 | 9篇 |
1994年 | 8篇 |
1993年 | 20篇 |
1992年 | 19篇 |
1991年 | 17篇 |
1990年 | 14篇 |
1989年 | 18篇 |
1988年 | 14篇 |
1987年 | 12篇 |
1986年 | 7篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1979年 | 2篇 |
1976年 | 2篇 |
1975年 | 7篇 |
1974年 | 2篇 |
1973年 | 5篇 |
1966年 | 2篇 |
1964年 | 1篇 |
1963年 | 2篇 |
排序方式: 共有1544条查询结果,搜索用时 31 毫秒
991.
Choemon Kanno Kunio Yamauchi Tomokichi Tsugo 《Bioscience, biotechnology, and biochemistry》2013,77(12):2067-2073
The polar lipid material which contains most of unsaponiriable matter of milk fat was collected by means of neutral alumina column chromatography. After saponification of the polar lipid material, the unsaponiriable matter was purified by repeated Florisil and neutral alumina column chromatography and the total tocopherol fraction was obtained. It was found that the total tocopherol fraction isolated from milk fat contained 6 of the known naturally occurring tocopherols, that is, α-, β-, γ-, and δ-tocopherols and α- and γ-tocotrienols. These were identified by two-dimensional thin-layer and gas-liquid chromatography before and after hydrogenation. 相似文献
992.
Katsuichi Saito Toshihide Nakamura Isao Kobayashi Mayumi Ohnishi-Kameyama Hitomi Ichinose Keitarou Kimura 《Bioscience, biotechnology, and biochemistry》2013,77(12):2120-2127
Some Lactobacillus brevis strains were found to aggregate upon the addition of xylan after screening for lactic acid bacteria that interact with plant materials. The S-layer proteins of cell surface varied among the strains. The strains that displayed xylan-mediated aggregation retained its ability even after the removal of S-layer proteins. L. brevis had negative zeta potentials. A correlation between the strength of aggregation and zeta potential was not observed. However, partial removal of S-layer proteins resulted in decreases in the electric potential and aggregation ability of some strains. Therefore, xylan-mediated aggregation of L. brevis was considered to be caused by an electrostatic effect between the cells and xylan. L. brevis also aggregated in the presence of mucin, and the strengths of aggregation among the strains were similar to that induced by xylan. Thus, xylan- and mucin-mediated L. brevis aggregation was supposed to be caused by a similar mechanism. 相似文献
993.
Choemon Kanno Hiroyuki Hattori Kunio Yamauchi 《Bioscience, biotechnology, and biochemistry》2013,77(5):1325-1332
Properties of proteins in the light (<d 1.03 on Ficoll and then <d 1.14 on sucrose) and heavy (d 1.03/1.05 on Ficoll and then <d 1.14 on sucrose) plasma membranes (PM) isolated from lactating bovine mammary gland were investigated. The PMs consisted of 57 ~ 62% of protein and 38 ~ 43% lipid. The lipid/protein ratio was 0.77 in the light PM and higher than 0.61 of the heavy PM. However, no appreciable differences were found between the light and heavy PMs with respect to polypeptide, amino acid, and carbohydrate compositions. The protein moiety contained approximate 5 ~ 6% carbohydrate: fucose 8 ~ 9, mannose 11 ~ 12, galactose 16, N-acetylglucosamine 32,N-acetylgalactosamine 12 ~ 15, and sialic acid 17~20 mol%. PM protein was high in the content of aspartic acid, glutamic acid, and leucine and low in proline, cystine, methionine, and histidine. On double immunodiffusion both PMs formed precipitin lines against milk fat globule membrane (MFGM) antiserum. Electrophoretic analysis on sodium dodecyl sulfate-polyacrylamide gel revealed the presence of many minor polypeptides and three major glycopeptides (PAS-I, II, and III) of molecular weights of 115,000, 94,000, and 82,000. The glycoprotein profiles of the PMs were different from those of MFGM, except that PAS-II and -III of PM corresponded to PAS-3 and -4 of MFGM, respectively. 相似文献
994.
Choemon Kanno Hiroyuki Hattori Kunio Yamauchi 《Bioscience, biotechnology, and biochemistry》2013,77(11):2995-3001
The light and heavy plasma membranes (PM) isolated from lactating bovine mammary glands contained 38~43% lipid of which 41~44% was phospholipid and 47~52% neutral lipid. The contents of phospholipid and neutral lipid were somewhat higher in the light PM than in the heavy PM. Cholesterol was contained 55 ~60% of neutral lipid and the ratio of cholesterol to phospholipid was 0.64 to 0.69. Phospholipid was composed of sphingomyelin (Sph) 29~38%, phosphatidylcholine (PC) 27~35%, phosphatidylethanolamine (PE) 16~20%, phosphatidylserine 10%, and phosphatidylinositol 6~7%. The content of Sph was higher in the heavy PM than in the light PM, while the values of PC and PE were opposite. The major fatty acids of lipid components were palmitic acid, stearic acid, and oleic acid and those of Sph were palmitic acid, stearic acid, C23:0 and 24:0. The fatty acid composition of individual lipid classes differed significantly from each other but were similar between the light and heavy PMs. Tetracosapentaenoic acid (C24:5) was the major fatty acid of the diacylglycerol fraction. The results indicated that the lipid composition, especially phospholipid components, of bovine mammary gland PMs was different from those of milk fat globule membranes which is derived from the PM of mammary secretory cells. 相似文献
995.
996.
Miho Itoh Hideki Tatsukawa Lee Eun-Seo Kiyofumi Yamanishi Soichi Kojima Kiyotaka Hitomi 《The journal of histochemistry and cytochemistry》2013,61(11):793-801
Transglutaminase (TG) is a family of enzymes that catalyzes cross-linking reactions among proteins. Using fluorescent-labeled highly reactive substrate peptides, we recently developed a system to visualize isozyme-specific in situ enzymatic activity. In the present study, we investigated the in situ activities of TG1 (skin-type) and TG2 (tissue-type) using whole mouse sections of various embryonic developmental stages and neonates. In each case, we also successfully used immunostaining of identical whole mouse sections for protein expression after detection of enzymatic activities. In general, the enzymatic activity was correlated with TG protein expression. However, in some tissues, TG protein expression patterns, which were inconsistent with the enzymatic activities, suggested that inactive TGs were produced possibly by self cross-linking or other modifications. Our method allowed us to simultaneously observe developmental variations in both TG isozyme-specific activities and protein levels in mouse embryonic and neonate tissues. 相似文献
997.
998.
Structural requirements of strigolactones for germination induction and inhibition of Striga gesnerioides seeds 总被引:1,自引:0,他引:1
Saki Nomura Hitomi Nakashima Masaharu Mizutani Hirosato Takikawa Yukihiro Sugimoto 《Plant cell reports》2013,32(6):829-838
Key message
Structure–activity relationship studies of strigolactones and Striga gesnerioides seed germination revealed strict structural requirements for germination induction and a new function of the plant hormones as germination inhibitors.Abstract
Stereoisomers of the naturally occurring strigolactones, strigol, sorgolactone, orobanchol, sorgomol and 5-deoxystrigol, 36 in total, were prepared and screened for the ability to induce and/or inhibit the germination of Striga hermonthica and Striga gesnerioides seeds collected from mature plants that parasitized on sorghum and cowpea, respectively. All of the compounds induced S. hermonthica seed germination, albeit displayed differential activities. On the other hand, only a limited number of the compounds induced significant germination in S. gesnerioides, thus indicating strict structural requirements. Strigolactones inducing high germination in S. gesnerioides induced low germination in S. hermonthica. Strigolactones with the same configuration at C3a, C8b and C2′ as that in 5-deoxystrigol (9a) induced high germination of S. hermonthica seeds, but most of them inhibited the germination of S. gesnerioides. The differential response of S. gesnerioides to strigolactones may play an important role in the survival of the species. However, the compounds could be used as means of control if mixed cropping of cowpea and sorghum is adopted. 相似文献999.
1000.
Akira Hirono Hisaichi Fujii Kazue Hirono Hitoshi Kanno Shiro Miwa 《Human genetics》1992,88(3):347-348
Summary The entire coding sequence of a Japanese class 1 variant (G6PD Tokyo) was amplified by the polymerase chain reaction from genomic DNA. Nucleotide analysis by a direct sequencing technique revealed a unique nucleotide substitution (1246 G to A) in exon 10, which predicts a Glu to Lys substitution at the 416th amino acid. This is another member of a conspicuous mutation cluster surrounding the putative NADP-binding domain. 相似文献