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991.
K Fujiwara T Tamura F Taguchi K Machii K Suzuki 《Comptes rendus des séances de la Société de biologie et de ses filiales》1976,170(2):509-513
Nude (nu/nu) mice failed to resist to virulent mouse hepatitis virus (MHV) infection after vaccination with inactivated virus. Resistance was induced in nu/nu mice by the transfer of spleen cells from heterozygous haired (nu/ +) mice concomitantly with the vaccination, and the effect was more remarkable with spleen cells from immunized nu/ + mice. Antibody was demonstrable in nu/nu mice having received nu/ + cells and survived challenge infection. 相似文献
992.
The superior cervical ganglion was reinnervated by vagal afferent fibers following heterologous cross anastomosis between the superior cervical preganglionic trunk and the vagal trunk at the level of the supranodose ganglion in cats. The contractions of the nictitating membrane and the postganglionic action potentials from the external carotid sinus nerve in response to electrical stimulation of the vagal artificial preganglionic trunk in these operated cats were inhibited by treatment with tetraethylammonium and atropine. The choline acetyltransferase activities were measured by the radiometric method. The activities in cross anastomosed superior cervical ganglion were lower than those of normal superior cervical ganglion, but higher than those of chronically decentralized superior cervical ganglion. The activities in cross anastomosed nodose ganglion were lower than those of normal nodose ganglion, but higher than those of chronically decentralized superior cervical ganglion. These results further support the view that the primary afferent vagus artificially synapsed in the superior cervical ganglion is cholinergic. 相似文献
993.
A designed mRNA consisting of 42 ribonucleotides having the cap structure was synthesized. The capped leader sequence of the brome mosaic virus (BMV) mRNA 4, m7G5'pppGUAUUAAUA (F-1), was synthesized by the phosphotriester method and followed by the capping reaction. A 32-mer consisting of an initiation codon (AUG), the coding region corresponding to a bacterial pheromone cAD1 and two stop codons, was constructed by the 18-mer (F-2) and 14-mer (F-3), which were synthesized by the phosphoramidite method. 2'-,3'-O-Methoxymethylene-guanosine 5'-phosphate was condensed with F-3 using P1-2',3'-O-methoxymethyleneguanosine-5'-yl P2-adenosine-5'-yl pyrophosphate (9) with T4 RNA ligase. The chemically synthesized RNA fragments were ligated successively with T4 RNa ligase to afford the whole RNA molecule. 相似文献
994.
Repair of scalp defects using a tissue expander and Marlex mesh. 总被引:3,自引:0,他引:3
A simple technique using Marlex mesh and a tissue expander to cover scalp defects is described and two patients are presented. This technique is suitable for medium-sized defects that cannot be closed primarily. Marlex mesh is sutured to the wound edges in lieu of a temporary skin graft and to prevent enlargement of the defect during tissue expansion. The tissue expander is placed under adjacent normal scalp in a subgaleal pocket developed through the scalp defect. The scalp defect is closed secondarily using the expanded scalp flap. This technique was performed in two patients with satisfactory results. Marlex mesh obviates the need for a temporary skin graft to cover the scalp defect. 相似文献
995.
Cystic fibrosis mutations in North American populations of French ancestry: Analysis of Quebec French-Canadian and Louisiana Acadian families 总被引:4,自引:2,他引:2 下载免费PDF全文
Rima Rozen Robert H. Schwartz Bettina C. Hilman Pat Stanislovitis Glenn T. Horn Katherine Klinger Jocelyne Daigneault Marc De Braekeleer Bat-sheva Kerem Lap-Chee Tsui T. Mary Fujiwara Kenneth Morgan 《American journal of human genetics》1990,47(4):606-610
A 3-bp deletion (ΔF508) in the cystic fibrosis (CF) gene is the mutation on the majority of CF chromosomes. We studied 112 CF families from North American populations of French ancestry: French-Canadian families referred from hospitals in three cities in Quebec and from the Saguenay-Lac St. Jean region of northeastern Quebec and Acadian families living in Louisiana. ΔF508 was present on 71%, 55%, and 70% of the CF chromosomes from the major-urban Quebec, Saguenay-Lac St. Jean, and Louisiana Acadian families, respectively. A weighted estimate of the proportion of ΔF508 in the French-Canadian patient population of Quebec was 70%. We found that 95% of the CF chromosomes with ΔF508 had D7S23 haplotype B, the most frequent haplotype on CF chromosomes. In the Saguenay-Lac St. Jean families, 86% of the CF chromosomes without ΔF508 had the B haplotype, compared with 31% for the major-urban Quebec and Louisiana Acadian families. The incidence of CF in the Saguenay-Lac St. Jean population was 1/895 live-born infants. 相似文献
996.
ADP-ribosylation of the rho/rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells 总被引:12,自引:0,他引:12
T Nishiki S Narumiya N Morii M Yamamoto M Fujiwara Y Kamata G Sakaguchi S Kozaki 《Biochemical and biophysical research communications》1990,167(1):265-272
Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho/rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyltransferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho/rac proteins by ADP-ribosylation to induce these changes in the cells. 相似文献
997.
Yoshisada Fujiwara 《Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression》1982,699(3):217-225
Crosslink repair of mitomycin C-induced interstrand crosslinks was studied in exponentially growing and confluent normal human, transformed WI38CT-1, Fanconi's anemia (FA) and xeroderma pigmentosum (XP) group-A fibroblasts by the assay methods of alkaline sucrose centrifugation, hydroxyapatite column chromatography and S1-nuclease digestion. These three methods demonstrated unequivocally that crosslinking occurred at a rate of 0.13 crosslinks/108 Da per μg per ml mitomycin C ( 10 μg/ml) and the first half-excision of crosslinks followed the rapid first-order kinetics of 2–3 h half-life in exponentially-growing normal, WI38CT-1 and XP group-A cells. However, the first half-excision was completely defective in three out of the four FA strains tested and severely retarded in an FA strain. These results strongly support our previous observations in different strains of normal human, FA and XP group-A cells. An important new addition is that confluent, otherwise proficient, normal and XP cells almost completely lost the ability of the first, rapid half-excision of mitomycin C crosslinks in their DNA. This probably suggests that the enzyme or regulatory factor responsible for the half-excision, which differs from that for nucleotide excision repair, present constitutively in confluent cells, may be induced or activated only in the cycling cells. However, its relation to a defective FA factor is not clear at present. 相似文献
998.
M. Kurokawa S. Oda E. Tsubotani H. Fujiwara K Yokoyama S. Ishibashi 《Molecular and cellular biochemistry》1982,45(3):151-157
Summary A difference was observed in the intracellular distribution between type I and II hexokinases in Ehrlich-Lettre hyperdiploid ascites tumor cells (ELD cells). Experiment of the rebinding to the mitochondria for either each or mixture of the partially purified preparations of the two types of hexokinase indicated that the accepting site on the mitochondrial membrane was common for both types. Mild treatment of the two isoenzymes with chymotrypsin resulted in loss of the binding ability to mitochondria without change in the catalytic activity. It was deduced from these results that the essential region in the two types of hexokinase to interact with mitochondria, which was cleaved by chymotrypsin, was the same or near-similar.Secondly, rebinding to and releasing from mitochondria were examined for the two hexokinase isoenzymes in the presence of various factors affecting the interaction between hexokinase and mitochondria, such as divalent cations, glucose 6-phosphate, and Pi. In the absence of divalent cations, about a half of the type I isoenzyme was bound to mitochondria, whereas almost no type II was bound. A difference was also seen between the two types in the concentration of divalent cations required for the saturation of the binding. A more marked difference was observed in the effect of Pi either alone or in combination with glucose 6-phosphate on the activity and binding ability of the two hexokinases. For type I isoenzyme, Pi relieved both inhibitory and releasing effects of glucose 6-phosphate. On the contrary, for type II, Pi had no such a modulating effect on the releasing action of glucose 6-phosphate, and had the inhibitory effect for itself on the enzyme activity.From these results, it is likely that the difference in the intracellular distribution between type I and II hexokinases in ELD cells is due to the difference in their catalytic regions in the reaction with these ligands, which would induce the structural change in the region responsible for the binding to mitochondria. 相似文献
999.
Toshikazu Yamanouchi Toshio Tsushima Hitomi Murakami Yuji Sato Kazuo Shizume Kazuo Oshimi Hideaki Mizoguchi 《Biochemical and biophysical research communications》1982,108(1):414-420
Changes in insulin receptors accompanying cell differentiation in human promyelocytic leukemia cells (HL-60) were studied. Cell differentiation was induced by 1α,25-dihydroxyvitamin D3, vitamin A, dimethyl sulfoxide, or phorbol esters. 1α,25-dihydroxy-vitamin D3 increased the ability of HL-60 cells to bind insulin in a dose-dependent manner. The increase in insulin binding was due to an increase in the number of insulin receptors. Vitamin A, dimethyl sulfoxide and phorbol esters were also effective in increaseing insulin receptors. Thus, the differentiation of HL-60 cells was accompanied by an increase in insulin receptors. 相似文献
1000.
Trigeminal nerve: the possible origin of substance p-nergic response in isolated rabbit iris sphincter muscle 总被引:2,自引:0,他引:2
We determined the effects of trigeminal nerve denervation on the noncholinergic, nonadrenergic response to electrical transmural stimulation of the isolated rabbit iris sphincter muscle. The left ophthalmic nerve (first branch of the trigeminal nerve) was cut at the intracranial, peripheral site of the trigeminal ganglion and five to ten days later, the iris sphincter muscle isolated from the left eye (operated side) was found to produce a fast cholinergic contraction in response to electrical transmural stimulation and there was no evidence of noncholinergic, nonadrenergic contractions. On the other hand, in the iris sphincter muscle isolated from the right eye (control side), electrical transmural stimulation produced both cholinergic and noncholinergic, nonadrenergic contractile responses. Capsaicin and bradykinin produced noncholinergic, nonadrenergic contractile responses in the muscle from the control side, while in the iris sphincter from the trigeminally denervated eye there was no such response to application of these drugs. Exogenous substance P (SP) and carbachol produced a strong contractile response in both the trigeminally innervated and denervated sphincter muscles. Somatostatin, vasoactive intestinal polypeptide (VIP) and enkephalin were without effects. These observations suggest that the noncholinergic, nonadrenergic responses to electrical transmural stimulation are derived from the trigeminal nerve and that the mediator involved is probably SP or a related peptide. 相似文献