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21.
Quantum dots (QDs) are novel photostable semiconductor nanocrystals possessing wide excitation spectra and narrow, symmetrical emission spectra and can be conjugated to a wide range of biological targets, including proteins, antibodies and nucleic acid probes. These characteristics have provoked considerable interest in their use for bioimaging. Much investigation has been performed into their use for multiplex immunohistochemistry and in situ hybridisation which, when combined with multispectral imaging, has enabled quantitation and colocalisation of gene expression in clinical tissue. Many advances have recently been made using QDs for live cell and in vivo imaging, in which QD-labelled molecules can be tracked and visualised in 3-D. This review aims to outline the beneficial properties presented by QDs along with important advances in their biological application. 相似文献
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Host mediated selection of pathogen genotypes as a mechanism for the maintenance of baculovirus diversity in the field 总被引:1,自引:0,他引:1
Hitchman RB Hodgson DJ King LA Hails RS Cory JS Possee RD 《Journal of invertebrate pathology》2007,94(3):153-162
The genetic diversity of many DNA virus populations in nature is unknown, but for those that have been studied it has been found to be relatively high. This is particularly true for baculoviruses, a family of large double-stranded DNA viruses that infect the larval stages of insects. Why there should be such heterogeneity within these virus populations is puzzling and what sustains it is still unknown. It has long been recognized that some baculoviruses have a relatively wide host range, but the effect of different host species on the genotypic structure of a baculovirus population has received little attention. We provide evidence that infection of different insect species can influence the genetic diversity of a Panolis flammea nucleopolyhedrovirus (PaflNPV) population, isolated from the pine beauty moth. Variable regions of the PaflNPV genome were sequenced and novel ORFs were identified on each of the enlarged fragments. The roles of these orfs and the implications of their presence or absence within different genotypes are discussed. The variable fragments were also labelled with 32P and used as polymorphic genetic markers of genotype abundance. The proportion of polymorphic loci changed after passage in different insect species and this varied among species, suggesting a role for host selection of pathogen genotypes in the field as a mechanism for maintaining genetic diversity. These results have wide-ranging implications for understanding the ecology of insect-virus interactions in the natural environment and the evolution of baculovirus life history strategies. 相似文献
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Emotional intelligence (EI) is a multi-faceted construct consisting of our ability to perceive, monitor, regulate and use emotions. Despite much attention being paid to the neural substrates of EI, little is known of the spontaneous brain activity associated with EI during resting state. We used resting-state fMRI to investigate the association between the amplitude of low-frequency fluctuations (ALFFs) and EI in a large sample of young, healthy adults. We found that EI was significantly associated with ALFFs in key nodes of two networks: the social emotional processing network (the fusiform gyrus, right superior orbital frontal gyrus, left inferior frontal gyrus and left inferior parietal lobule) and the cognitive control network (the bilateral pre-SMA, cerebellum and right precuneus). These findings suggest that the neural correlates of EI involve several brain regions in two crucial networks, which reflect the core components of EI: emotion perception and emotional control. 相似文献
24.
B M Arnold M Kuttner D M Willis A J Hitchman J E Harrison T M Murray 《Canadian journal of physiology and pharmacology》1975,53(6):1135-1140
Using a specific radioimmunoassay for porcine intestinal calcium-binding protein (CaBP), we have measured the concentration of CaBP in the various tissues and organs of normal pigs. Intestinal CaBP was present in highest concentration in the upper small intestine, with lower concentrations in the distal small intestine. Intestinal CaBP was also found, in lower concentrations, in kidney, liver, thyroid, pancreas, and blood. In all other tissues, including parathyroid, bone, skeletal muscle, and brain, CaBP immunoreactivity was undetectable or less than in blood. The elution profile of calcium-binding activity and immunoreactivity from gel filtration analysis of kidney and parathyroid extracts suggest that the calcium-binding protein in the parathyroid gland, and the major calcium-binding protein(s) in the kidney, are chemically and immunochemically different from intestinal CaBP. 相似文献
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Lotus corniculatus nodulation specificity is changed by the presence of a soybean lectin gene 总被引:5,自引:0,他引:5
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Plant lectins have been implicated as playing an important role in mediating recognition and specificity in the Rhizobium-legume nitrogen-fixing symbiosis. To test this hypothesis, we introduced the soybean lectin gene Le1 either behind its own promoter or behind the cauliflower mosaic virus 35S promoter into Lotus corniculatus, which is nodulated by R. loti. We found that nodulelike outgrowths developed on transgenic L. corniculatus plant roots in response to Bradyrhizobium japonicum, which nodulates soybean and not Lotus spp. Soybean lectin was properly targeted to L. corniculatus root hairs, and although infection threads formed, they aborted in epidermal or hypodermal cells. Mutation of the lectin sugar binding site abolished infection thread formation and nodulation. Incubation of bradyrhizobia in the nodulation (nod) gene-inducing flavonoid genistein increased the number of nodulelike outgrowths on transgenic L. corniculatus roots. Studies of bacterial mutants, however, suggest that a component of the exopolysaccharide surface of B. japonicum, rather than Nod factor, is required for extension of host range to the transgenic L. corniculatus plants. 相似文献
27.
The large N-linked oligosaccharides released from Schizosaccharomyces pombe
by endo-beta-N-acetylglucosaminidase H were examined to determine how the
negatively chargedpyruvylated galactoses present (Gemmill,T.R., and
Trimble,R.B., 1996, J. Biol. Chem ., 271, 25945-25949) were attached to the
oligosaccharide chains. Binding of biotinylated human serum amyloid P and
peanut agglutinin to native and depyruvylated S.pombe glycoproteins,
respectively, indicated that the pyruvylated epitope was likely to be in
the beta configuration. Examination by high- field 1H NMR of whole glycans
and a disaccharide fragment released from them on partial acid hydrolysis
showed that the pyruvylated galactose species was in fact beta1,3-linked to
a second galactose, and this occurred an average of five to six times on
nominal Gal57Man64GlcNAc N- glycans. The pyruvate-2,(4,6)Gal-beta1,3Gal
epitope is chemically similar to acetaldehyde-Galbeta1,3Gal groups found on
the glycoproteins from Paramyxovirus-infected bovine kidney cells (Prehm,
P., Scheid,A. and Choppin,P.W. ,1979, J. Biol. Chem ., 254, 9669-9677). The
1:1 stoichiometry between pyruvate and beta-linked galactose in these
S.pombe glycans indicates that either pyruvate addition to terminal
beta1,3Gal is highly efficient or that pyruvylated Gal is transferred en
bloc to alpha1,2-linked Gal residues in theN-linked chains. In
contradiction to many galactomannan-producing fungi, which add substantial
amounts of Gal in the furanose form to their glycoproteins, all detectable
Gal in the large S.pombe galactomannans is in the pyranose form, as found
in higher eukaryotes. The current work shows that the S.pombe outer chain
structure is a poly-alpha1,6Man backbone 2- O-substituted with either Gal
or the pyruvylated galactobiose and contains little alpha1,2-linked or
2-O-substituted Man. This is in contrast to the S. cerevisiae outer chain,
which is poly-alpha1,6Man substituted with alpha1,2-linked Man sidechains
(Ballou,C.E. ,1990, Methods Enzymol , 185, 440-470).
相似文献
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Hodgson DJ Hitchman RB Vanbergen AJ Hails RS Possee RD Cory JS 《Journal of evolutionary biology》2004,17(5):1018-1025
Mixed‐genotype infections are common in many natural host–parasite interactions. Classical kin‐selection models predict that single‐genotype infections can exploit host resources prudently to maximize fitness, but that selection favours rapid exploitation when co‐infecting genotypes share limited host resources. However, theory has outpaced evidence: we require empirical studies of pathogen genotypes that naturally co‐infect hosts. Do genotypes actually compete within hosts? Can host ecology affect the outcome of co‐infection? We posed both questions by comparing traits of infections in which two baculovirus genotypes were fed to hosts alongside inocula of the same or a different genotype. The host, Panolis flammea, is a herbivore of Pinus sylvestris and Pi. contorta. The pathogen, PfNPV (a nucleopolyhedrovirus), occurs naturally as mixtures of genotypes that differ, when isolated, in pathogenicity, speed of kill and yield. Single‐genotype infection traits failed to predict the ‘winning’ genotypes in co‐infections. Co‐infections infected and caused lethal disease in more hosts, and produced high yields, relative to single‐genotype infections. The need to share with nonkin did not cause fitness costs to either genotype. In fact, in hosts feeding on Pi. sylvestris, one genotype gained increased yields in mixed‐genotype infections. These results are discussed in relation to theory surrounding adaptive responses to competition with nonkin for limited resources. 相似文献
30.
Congcong Liu Zhencai Chen Ting Wang Dandan Tang Glenn Hitchman Jiangzhou Sun Xiaoyue Zhao Lijun Wang Antao Chen 《PloS one》2015,10(5)
The Stroop effect is one of the most robust and well-studied phenomena in cognitive psychology and cognitive neuroscience. However, little is known about the relationship between intrinsic brain activity and the individual differences of this effect. In the present study, we explored this issue by examining whether resting-state functional magnetic resonance imaging (rs-fMRI) signals could predict individual differences in the Stroop effect of healthy individuals. A partial correlation analysis was calculated to examine the relationship between regional homogeneity (ReHo) and Stroop effect size, while controlling for age, sex, and framewise displacement (FD). The results showed positive correlations in the left inferior frontal gyrus (LIFG), the left insula, the ventral anterior cingulate cortex (vACC), and the medial frontal gyrus (MFG), and negative correlation in the left precentral gyrus (LPG). These results indicate the possible influences of the LIFG, the left insula, and the LPG on the efficiency of cognitive control, and demonstrate that the key nodes of default mode network (DMN) may be important in goal-directed behavior and/or mental effort during cognitive control tasks. 相似文献