ABSTRACT: INTRODUCTION: Valproic acid is a commonly used anti-epileptic drug. Hematological toxicities are among theoccasionally observed adverse effects of this medication. CASE PRESENTATION: We present the case of a 13-year-old Caucasian boy who demonstrated mild anemia 12months after the introduction of valproic acid therapy. A bone marrow biopsy revealedmaturation arrest of proerythroblasts. CONCLUSION: Prompt diagnosis and valproic acid discontinuation resulted in the patient's recovery. 相似文献
In January-February 1991, in Prydz Bay, phytoplankton bloomwas evident in the inner shelf area with the dominant diatomsbeing represented mainly by pennate species of the Nitzschia-Fragilariopsisgroup. Dinoflagellates and naked flagellates were most abundantin the centre of the bay; however, larger heterotrophic speciesprevailed at the southern stations. Cell carbon values (average317 µg l1; range 92-1048 µg l1) foundin the bloom in the south were chiefly due to pennate diatomsand larger heterotrophic dinoflagellates. Much lower carbonvalues (average 51 µg l1; range 7-147 µgl1) in the outer shelf region were mainly contributedby large centric diatoms (70-110 mu;m) and small dinoflagellates(5-25 µm). Wide ranges of algal cell sizes were observedin both southern and northern communities; the overlapping ofsizes of diatoms and flagellates, the latter containing heterotrophs,suggested complex trophic relationships within the planktonand an enhanced heterotrophic activity in the south. North-to-southvariations in surface 相似文献
The novel peptidasome, called presequence protease, PreP, was originally identified and characterized in Arabidopsis thaliana as a mitochondrial matrix and chloroplast stroma localized metalloprotease. PreP has a function as the organellar peptide clearing protease and is responsible for degrading free targeting peptides and also other unstructured peptides up to 65 amino acid residues that might be toxic to organellar functions. PreP contains an inverted Zn-binding motif and belongs to the pitrilysin protease family. The crystal structure of AtPreP refined at 2.1 Å demonstrated a unique totally enclosed large cavity of 10 000 Å3 that opens and closes in response to peptide binding, revealing a novel catalytic mechanism for proteolysis. Homologues of PreP have been found in yeast and human mitochondria. Interestingly, the human PreP, hPreP, is the protease that is responsible for clearing the human brain mitochondria from the toxic amyloid-β peptide (Aβ) associated with Alzheimer's disease (AD). Accumulation of Aβ has been shown in the brain mitochondria from AD patients and mutant transgenic mice overexpressing Aβ. Here, we present a review of our present knowledge on structural and functional characteristics of PreP and discuss its mitochondrial Aβ-degrading activity in the human brain mitochondria in relation to AD. 相似文献
iSERS (SERS=surface‐enhanced Raman scattering) microscopy is an emerging Raman‐based staining technique for the selective localization of target proteins on cells and tissues using antibody‐ SERS nanotag conjugates. In this contribution we demonstrate the feasibility of iSERS for imaging of programmed cell death‐ligand 1 (PD‐L1), an important predictive biomarker, on single SkBr‐3 breast cancer cells. Further details can be found in the article by Elzbieta Stepula, Matthias König, Xin‐Ping Wang, et al. ( e201960034 ).
Low molecular weight protein kinase from maize seedlings was isolated. The molecular weight of the enzyme was below 10 000. The enzyme preferentially utilized ATP and casein as donor and acceptor of phosphorus, respectively. Casein kinase was a cyclic nucleotide-independent, heparin-sensitive enzyme. The low molecular weight casein kinase was resolved into basic, neutral and slightly acidic peaks of activity by chromatofocussing. 相似文献
Genetic variants of serum alkaline phosphatase were studied by the method of starch gel electrophoresis in the Zlotnicka Pstra breed of pigs. Two regions of alkaline phosphatase migration were observed. A single fraction in region I and four different phenotypes: AB, B, BC and BD in region II, were found. For AB, B and BC phenotypes the genetic control by three alleles AkpA, AkpB and AkpC in suggested. The observed segregation ratios in some cases deviated significantly from the expected ones. 相似文献