全文获取类型
收费全文 | 538篇 |
免费 | 30篇 |
出版年
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 11篇 |
2016年 | 8篇 |
2015年 | 5篇 |
2014年 | 14篇 |
2013年 | 120篇 |
2012年 | 25篇 |
2011年 | 25篇 |
2010年 | 11篇 |
2009年 | 9篇 |
2008年 | 15篇 |
2007年 | 20篇 |
2006年 | 23篇 |
2005年 | 16篇 |
2004年 | 17篇 |
2003年 | 17篇 |
2002年 | 20篇 |
2001年 | 19篇 |
2000年 | 20篇 |
1999年 | 11篇 |
1997年 | 6篇 |
1994年 | 6篇 |
1992年 | 8篇 |
1991年 | 8篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 9篇 |
1984年 | 2篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1980年 | 2篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 9篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 7篇 |
1971年 | 4篇 |
1970年 | 9篇 |
1969年 | 9篇 |
1968年 | 7篇 |
1967年 | 4篇 |
1966年 | 3篇 |
1965年 | 2篇 |
排序方式: 共有568条查询结果,搜索用时 15 毫秒
521.
The site of action of antimycin A is known to lie between cytochrome b and c in the respiratory chain of mammalian cells. But in general, bacteria, even those which have cytochromes similar to those of mammalian cells such as Bacillus subtilis, are naturally resistant to this antibiotic.The mechanism of this natural resistance is studied using a strain of B. subtilis. Succinoxidase activity of the intact cells of this bacterium showed very low sensitivity to the antibiotic, but on disruption of the cells, the sensitivity increased 7.5 times. Moreover, the activity of the intact cells could be sensitized by treatment with cationic detergent. In addition to the permeability barrier suggested by the above results, it was found that the electron transport system of this bacterium contained antimycin A insensitive by-path. 相似文献
522.
Akira Yanai Teruhiko Beppu Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(7):1307-1311
A new strain of bacteriophage, phage 95, specific to Pseudomonas aeruginosa and Ps. schuylkilliensis was isolated. It has been shown that the phage induces a lytic enzyme which hydrolyzes peptide bond between l-alanine and d-glutamic acid in the peptidoglycan of cell wall.Here the characteristics of phage 95 are described. It possesses a hexagonal head of 650Å, a contractile tail of 1150Å and spike with fibers. Its DNA has a GC content of 48%, a density of 1.700 g · cm?3 and Tm of 87.7°C. Photoreactivation was observed. Latent period is 20 min and burst size is 50. The phage is stable between pH 5 and 7.5, and unstable above 55°C. Culture condition and 300 liter scale cultivation are also presented. 相似文献
523.
Tadayuki Hino Takeshi Uozumi Gakuzo Tamura Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(7):1109-1115
Mode of action of crystalline nuclease O obtained from autolyzed Aspergillus oryzae on RNA and synthetic homopolymers was examined. Crystalline nuclease O had no strict base specificity, although the velocity of hydrolysis was poly A > poly U > RNA > poly C. This enzyme did not degrade poly G. Digestion of high molecular weight RNA with an excess of this enzyme produced mono-, di- and trinucleotides with 5′-terminal phosphate. The amount of mono-, di- and trinucleotides was, respectively, 13.6, 70.0 and 16.4% of total degradation products. All the four bases were detected in mononucleotide fraction and 3′-terminals and 5′-terminals of oligonucleotides. 相似文献
524.
Yoshihiro Yoshiyama Kei Arima Michio Matsuhashi Gakuzo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(7):1573-1577
It was, using the particulate enzyme from Micrococcus lysodeikticus, revealed that most of 2-14C-mevalonic acid incorporated into the cell of Lactobacillus heterohiochii H-1 was incorporated into the lipid intermediate of cell wall biosynthesis. About 10% of the radioactivities incorporated into the cells was, however, found in nucleic acid fraction which was extracted from lysozyme treated cells with phenol. Most of the radioactivities in the nucleic acid fraction was eluted at the beginning of the elution pattern from Sephadex G-200 or MAK-column. The material is different from tRNA and rRNA. 相似文献
525.
Takeshi Uozumi Gakuzo Tamura Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(8):963-968
In was found that an intracellular ribonuclease was present as an inactive form in the fresh mycelium of Asp. oryzae. It was about 3 times activated either by 3 m urea or by the autolysis of mycelium at 30°C for 20 hr. The optimum pH of the ribonuclease activity was 8.3. It was heat sensitive (60°C, 10 min), and completely inhibited by 5 mm EDTA. It was activated by 1 mm Mg2+ and inhibited by Zn2+, Ca2+, Cd2+, Co2+ and Cu2+. 相似文献
526.
Keiichi Shimazaki Shintaro Sugai Ryoya Niki Shunrokuro Arima 《Bioscience, biotechnology, and biochemistry》2013,77(13):1995-2002
The secondary structure of bovine αs-casein and chemically modified αs-casein in various solvents was investigated by infrared absorption spectrum and optical rotatory dispersion measurements. Amino groups of αs-casein were either succinylated or acetylated, and carboxyl groups were either methylated or ethylated. Acetylated- and ethylated-αs-caseins are insoluble in water. Water-soluble samples have unordered structure in water. In organic solvents, such as 2-chloroethanol and ethylene glycol, they have about 50% α-helical fraction. On the other hand, it was found that methylated-αs-casein had two infrared absorption peaks centered at 1625 and 1643 cm?1 in D2O-CH3OD mixed solvent. This fact may be connected with the presence of β-structure. In the case of solid film of this sample, cast from solution containing CH3OH, the presence of β-structure was indicated, too. The authors attempted to explain the formation of β-structure in methylated-αs-casein in terms of the electrostatic interactions due to the differences in the net charge between methylated and unmodified αs-caseins. 相似文献
527.
Tsutomu Yamaguchi Gakuzo Tamura Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(5):519-521
A study was made of thermogravimetric analyses of microcrystalline cellulose, (Avicell), over a temperature range from 240°C to 300°C under air and nitrogen by means of a thermal balance. For comparative purpose, cellobiose and glucose were also used. The volatilization rate of cellulose was related to the amount of pyrolytic residue and accelerated owing to the oxidation in the presence of atmospheric oxygen. The apparent activation energies of pyrolysis were obtained from weight loss data.Quantities of carbonyl and carboxyl groups in pyrocellulose increased linearly against degradation stages of cellulose irrespective of pyrolytic temperatures and times. 相似文献
528.
Kei Arima Shinjiro Iwasaki Gakuzo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(5):540-551
Out of some 800 strains of microorganisms, a potent fungus for milk clotting enzyme was isolated from soil during the course of screening tests and was identified as one of strains of Mucor pusillus Lindt. Satisfactory results were obtained in cheese making experiments with this enzyme which could be produced effectively by solid culture on wheat bran at 30°C for about 70 hrs.The balance between milk clotting activity and proteolytic activity of this enzyme resembled very much to that of rennet.Microbial rennet from Mucor pusillus F-27 was obtained with high productivity by solid culture followed by water extraction. The enzyme could be precipitated by salting out with ammonium sulfate and also by mixing with various water-miscible organic solvents such as ethanol, methanol or acetone.This enzyme is one of acid proteases having its optimal pH for milk casein digestion around 3.5. The ratio of milk clotting activity to proteolytic activity of this enzyme resembled that of calf rennet than those of other proteases of fungal origin. This was more heat stable and more resistant against pH changes than animal rennet. Apparent activity of milk clotting was more affected by Ca ion concentration in milk than that of calf rennet.The liberation of 12% TCA soluble nitrogen from casein fraction was a little less specific than that of calf rennet. The optimal temperature for milk clotting lay around 56°C.Electrophoretic patterns of α-peak of casein treated with this enzyme showed the weak proteolysis which resembled that with rennet. 相似文献
529.
Naoki Higashi Hirofumi Shoun Keiji Yano Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(6):1081-1084
Sulfite ion (HSO3 -) is one of the products when elemental sulfur is oxidized by the hydrogen sulfide:ferric ion oxidoreductase of Thiobacillus ferrooxidans AP19-3. Under the conditions in which HSO3 - is accumulated in the cells, the iron oxidase of this bacterium was strongly inhibited by HSO3 -. Since cytochrome c oxidase is one of the most important components of the iron oxidase enzyme system in T. ferrooxidans, effects of HSO3 - on cytochrome c oxidase activity were studied with the plasma membranes of HSO3 --resistant and -sensitive strains of T. ferrooxidans, OK1-50 and AP19-3. The enzyme activity of AP19-3 compared with OK1-50 was strongly inhibited by HSO3 -. To investigate the inhibition mechanism of HSO3 - in T. ferrooxidans, cytochrome c oxidases were purified from both strains to an electrophoretically homogeneous state. Cytochrome c oxidase activity of a purified OK1-50 enzyme was not inhibited by 5 mM HSO3 -. In contrast, the same concentration of HSO3 - inhibited the enzyme activity of AP19-3 50%, indicating that the cytochrome c oxidase of OK1-50 was more resistant to HSO3 - than that of AP19-3. Cytochrome c oxidases purified from both strains were composed of three subunits. However, the molecular weight of the largest subunit differed between OK1-50 and AP19-3. Apparent molecular weights of the three subunits of cytochrome c oxidases were 53,000, 24,000, and 19,000 for strain AP19-3 and 55,000, 24,000, and 19,000 for strain OK1-50, respectively. 相似文献
530.
Atsushi Kakinuma Hiromu Sugino Masao Isono Gakuzo Tamura Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(6):973-976
Several properties of ATPase bound to the inner membrane of a psychrophilic marine bacterium Vibrio sp. strain ABE-1 were examined. The membrane-bound ATPase had two optimal peaks of the activity at pH 5.8 and 7.3. The ATPase activity was strongly inhibited by N,N’- dicyclohexylcarbodiimide (DCCD) and NaN3 at pH 5.8 and 8.0, and stimulated by MgCl2 and CaCl2 at pH 8.0. At pH 8.0, the enzyme hydrolyzed GTP and ITP as well as ATP but not AMP or p-nitrophenylphosphate. CTP, UTP, and ADP were poor substrates. These characteristics indicate that there is a F0F1-type ATPase in the inner membrane of this bacterium. In addition, the ATPase activity was also significantly inhibited by Na3 Vo4, suggesting the coexistence of a P-type ATPase as a minor constituent. The membrane-bound ATPase activity was maximum at 50°C, but the strong DCCD-sensitivity observed at 20°C was greatly reduced at this temperature. 相似文献