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排序方式: 共有95条查询结果,搜索用时 15 毫秒
91.
Yukio Kimura Noriko Yasuda Hiroko Tanigaki-Nagae Toshikatsu Nakabayashi Hisami Matsunaga Masako Kimura 《Bioscience, biotechnology, and biochemistry》2013,77(10):2509-2516
Acetoacetate decarboxylase is a valuable tool for clinical analysis of ketone bodies in human plasma. After screening many microorganisms, we found Bacillus polymyxa A-57 to be a new enzyme source with a good yield of acetoacetate decarboxylase. After purifying the intracellular enzyme by three-stage column chromatography, we identified it as a single protein by SDS-polyacrylamide gel electrophoresis. The enzyme had a molecular weight of approximately 280,000 and consisted of 10 (±2) identical subunits. It had an optimum pH of 5.9 and was stable up to about 60°C for 30min. The apparent Km and Vmax values for lithium acetoacetate were 0.94 mm and 296 μmiol/min/mg, respectively. In addition, the decarboxylase activity was found in the broth. After purification, we found that it was due to an active peptide which we named A-57-9, which we identified as the antibiotic polymyxin M1. However, the Vmax value (0.25 μmol/min/mg) of the peptide was very much lower and the Km value (400 mm) was higher than those of real acetoacetate decarboxylase. 相似文献
92.
Umemura M Yahagi A Hamada S Begum MD Watanabe H Kawakami K Suda T Sudo K Nakae S Iwakura Y Matsuzaki G 《Journal of immunology (Baltimore, Md. : 1950)》2007,178(6):3786-3796
IL-17 is a cytokine that induces neutrophil-mediated inflammation, but its role in protective immunity against intracellular bacterial infection remains unclear. In the present study, we demonstrate that IL-17 is an important cytokine not only in the early neutrophil-mediated inflammatory response, but also in T cell-mediated IFN-gamma production and granuloma formation in response to pulmonary infection by Mycobacterium bovis bacille Calmette-Guérin (BCG). IL-17 expression in the BCG-infected lung was detected from the first day after infection and the expression depended on IL-23. Our observations indicated that gammadelta T cells are a primary source of IL-17. Lung-infiltrating T cells of IL-17-deficient mice produced less IFN-gamma in comparison to those from wild-type mice 4 wk after BCG infection. Impaired granuloma formation was also observed in the infected lungs of IL-17-deficient mice, which is consistent with the decreased delayed-type hypersensitivity response of the infected mice against mycobacterial Ag. These data suggest that IL-17 is an important cytokine in the induction of optimal Th1 response and protective immunity against mycobacterial infection. 相似文献
93.
94.
Takashi Toyofuku Masayuki Suzuki Hisami Suga Saburo Sakai Atsushi Suzuki Tsuyoshi Ishikawa Lennart Jan de Nooijer Ralf Schiebel Hodaka Kawahata Hiroshi Kitazato 《Marine Micropaleontology》2011,78(3-4):113-120
Specimens of the benthic foraminifer Ammonia beccarii were cultured in the laboratory in order to determine the relation between temperature and Mg/Ca and oxygen isotope values in their tests. Asexual reproduction in this species provides a large number of juveniles that were allowed to grow into maturity at temperatures ranging from 10 to 27 °C and at salinities ranging from 18 to 33 PSU. The Mg/Ca in a calcite increase exponentially and δ18O decreases linearly with the temperature. Salinity has no significant impact on either Mg/Ca or δ18O. We show how the combination of these two parameters can be used to reconstruct seawater δ18O and temperature in shallow marine habitats. 相似文献
95.