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71.
In this article, we demonstrate that an increase in intracellular Ca2+ concentration may represent a specific common step(s) in the mechanism(s) of action of glutamate (Glu) and depolarizing agents on formation of inositol phosphates (IPs) in 8-day-old rat forebrain synaptoneurosomes. In fact, A23187, a Ca2+ ionophore, induces a dose-dependent accumulation of IPs, which is not additive with that evoked by Glu and K+ but is slightly synergistic with that induced by carbachol. In addition, Glu and K+ augment the intracellular Ca2+ concentration in synaptoneurosome preparations as measured by the fura-2 assay. The absence of external Ca2+ decreases basal and Glu-, and K(+)-stimulated formation of IPs. Cd2+ (100 microM) fully inhibits both Glu- and K(+)-evoked formation of IPs without affecting the carbachol-elicited response of IPs. Zn2+ inhibits Glu- and K(+)-stimulated accumulation of IPs (IC50 approximately 0.4 mM) but with a lower affinity than Cd2+ (IC50 approximately 0.035 mM). The organic Ca2+ channel blockers verapamil (10 microM), nifedipine (10 microM), omega-conotoxin (2 microM), and amiloride (10 microM) as well as the inorganic blockers Co2+ (100 microM) and La3+ (100 microM) block neither Glu- nor K(+)-evoked formation of IPs, a result suggesting that the opening of the L-, T-, N-, or P-type Ca2+ channels does not participate in these responses. All these data suggest that an increase in intracellular Ca2+ concentration resulting from an influx of Ca2+, sensitive to Cd2+ but not to other classical Ca2+ antagonists, may play a key role in the transduction mechanism activated by Glu or depolarizing agents. 相似文献
72.
73.
74.
Ammonium and nitrate uptake rates and rhizosphere pH in non-mycorrhizal roots of Norway spruce [Picea abies (L.) Karst.] 总被引:5,自引:0,他引:5
Summary Relationships between root zone temperature, concentrations and uptake rates of NH
4
+
and NO
3
–
were studied in non-mycorrhizal roots of 4-year-old Norway spruce under controlled environmental conditions. Additionally, in a forest stand NH
4
+
and NO
3
–
uptake rates along the root axis and changes in the rhizosphere pH were measured. In the concentration (Cmin) range of 100–150 M uptake rates of NH
4
+
were 3–4 times higher than those of NO
3
–
The preference for NH
4
+
uptake was also reflected in the minimum concentration (Cmin) values. Supplying NH4NO3, the rate of NO
3
–
uptake was very low until the NH
4
+
concentrations had fallen below about 100 M. The shift from NH
4
+
to NO
3
–
uptake was correlated with a corresponding shift from net H+ production to net H+ consumption in the external solution. The uptake rates of NH
4
+
were correlated with equimolar net production of H+. With NO
3
–
nutrition net consumption of H+ was approximately twice as high as uptake rates of NO
3
–
In the forest stand the NO
3
–
concentration in the soil solution was more than 10 times higher than the NH
4
+
concentration (<100 M), and the rhizosphere pH of non-mycorrhizal roots considerably higher than the bulk soil pH. The rhizosphere pH increase was particularly evident in apical root zones where the rates of water and NO
3
–
uptake and nitrate reductase activity were also higher. The results are summarized in a model of water and nutrient transport to, and uptake by, non-mycorrhizal roots of Norway spruce in a forest stand. Model calculations indicate that delivery to the roots by mass flow may meet most of the plant demand of nitrogen and calcium, and that non-mycorrhizal root tips have the potential to take up most of the delivered nitrate and calcium. 相似文献
75.
Karen A. Keith Mary C. Duncan Max Murray Ken Bairden Andrew Tait 《International journal for parasitology》1990,20(8):1037-1045
In this study we have shown that NHS-biotin and I125-streptavidin can detect cuticular polypeptides of Ostertagia spp. The labelled polypeptide profile of intact nematodes is simple compared to the profile obtained by labelling homogenates. None of the major internal polypeptides are labelled and the subset of proteins labelled in intact nematodes appears to be mainly surface associated. The results presented here demonstrate that NHS-biotin may be used as a reagent for the analysis of surface polypeptides. The surface polypeptide profiles of the five major developmental stages (L1, L2, L3, L4 and adult) of Ostertagia circumcincta show a series of stage-specific molecules with no polypeptides common to all stages, indicating that the cuticle is a dynamic structure which changes throughout the life cycle. Similarity comparison of Ostertagia ostertagi L3 and L4 stage surface profiles showed that each stage is clearly distinct; comparison of these stages between the two species shows an overall similarity. 相似文献
76.
77.
The effects of pentobarbitone on the transport of 45Ca2+ by rat brain mitochondria were studied, using the Ruthenium Red-EGTA quench technique. In the presence of succinate and inorganic phosphate, mitochondria rapidly accumulate 45Ca2+. Pentobarbitone (0.1-1.0 mM) stimulates the initial rate of Ca2+ transport. In contrast, pentobarbitone (1 mM) did not affect the NaCl (50 mM)-induced efflux of 45Ca2+ from mitochondria. Dibucaine (60 micro M), a clinically used local anaesthetic, inhibits both 45Ca2+ uptake an efflux. The results suggest that barbiturate stimulation of mitochondrial Ca2+ uptake may, in combination with effects on other Ca2+ sequestering processes, contribute to the inhibitor of transmitter release observed at a number of synapses. 相似文献
78.
Examination of the reproductive morphology of the adelphoparasitic red alga Gardneriella tuberifera Kylin reveals that this monotypic genus is correctly placed in the family Solieriaceae (Gigartinales), to which its host Agardhiella gaudichaudii (Montagne) Silva et Papenfuss also belongs. Gardneriella is multiaxial, nonprocarpic and has an inwardly directed, three-celled carpogonial branch. The large, reniform uninucleate auxiliary cell is distinct prior to and after fertilization. It is diploidized by an unbranched, multicellular connecting filament which lacks pit connections. One or two connecting filaments arise from each fertilized carpogonium. From the diploidized auxiliary cell, the gonimoblast initial is cut off obliquely toward the interior of the thallus. The cells of the gonimoblast fuse with adjacent unpigmented vegetative cells of Gardneriella and pigmented cells of the host. These cells become incorporated into the developing cystocarp and, from those of Gardneriella, additional short chains of gonimoblast cells arise. The mature cystocarp is placentate, radiately lobed, and lacks a surrounding involucre. Carposporangia are borne in short chains and the unpigmented carpospores are released upon the dissolution of outer vegetative cells. No ostiole is present. Gardneriella appears to be most closely related to the placentate solieriacean genera Agardhiella, Sarcodiotheca, and Meristiella and therefore this genus should be placed in the tribe recently erected for these taxa, the Agardhielleae. 相似文献
79.
The effects of regeneration on ecdysteroid levels and duration of the 10th larval instar of female house crickets were studied. Three experimental groups were used. The first consisted of larvae regenerating two legs cut off on the first day of this stage. The second group was made up of insects that underwent either an epidermal incision (sham-operated group I) or bleeding (sham-operated group II) at the same time as amputation in the first group. The last group was composed of normal insects.Larval stage length: in the first two groups, the duration of the 10th larval stage was significantly modified. It increased for regenerating animals as well as for sham-operated group I, but decreased for sham-operated group II.Ecdysteroid production: controls showed two periods of intense ecdysteroid activity. The first, which took place between days 4 and 6, was assumed to induce apolysis of the tegument and to render regeneration impossible (it coincided with the so-called critical period for regeneration). The second, which occurred between days 7 and 8 initiated cuticle synthesis. For sham-operated insects neither epidermal injury nor a loss of blood appear to change this hormonal profile significantly. Regenerating crickets however exhibited drastically reduced ecdysteroid peaks: the total apparent production of ecdysteriods was 50% below normal. Moreover, relative concentration of ecdysone to 20-hydroxy-ecdysone was greatly increased. Thus, regeneration obviously has a great and specific influence on ecdysteroid release in insect larvae. This effect may be related to changes observed in the prothoracic gland cycle which suggest that it plays a complex role in the regulation of the regenerative process. 相似文献
80.
Summary Myogenic cells from mice homozygous for the lethal mutation motor endplate disease (med/med) were grown in culture. Like muscle cells taken from wild type (+/?) litter mates they fused to form myotubes which contracted, developed cross striations, and exposed acetylcholine receptors (AChR) on their surface. However, a decrease of 30% in the number of mononucleated cells per unit fresh weight of muscle was observed as early as 2–3 days postnatal, i.e., at least one week prior to the onset of physiological symptoms. Hence, in addition to influencing the functional maintenance of motor endplates, the med gene seems to control early events in muscle development. 相似文献