全文获取类型
收费全文 | 9300篇 |
免费 | 510篇 |
国内免费 | 5篇 |
出版年
2023年 | 19篇 |
2022年 | 67篇 |
2021年 | 128篇 |
2020年 | 67篇 |
2019年 | 97篇 |
2018年 | 113篇 |
2017年 | 114篇 |
2016年 | 164篇 |
2015年 | 300篇 |
2014年 | 340篇 |
2013年 | 589篇 |
2012年 | 583篇 |
2011年 | 576篇 |
2010年 | 405篇 |
2009年 | 351篇 |
2008年 | 624篇 |
2007年 | 603篇 |
2006年 | 603篇 |
2005年 | 579篇 |
2004年 | 574篇 |
2003年 | 566篇 |
2002年 | 527篇 |
2001年 | 152篇 |
2000年 | 137篇 |
1999年 | 166篇 |
1998年 | 128篇 |
1997年 | 107篇 |
1996年 | 91篇 |
1995年 | 81篇 |
1994年 | 90篇 |
1993年 | 76篇 |
1992年 | 79篇 |
1991年 | 74篇 |
1990年 | 47篇 |
1989年 | 57篇 |
1988年 | 55篇 |
1987年 | 42篇 |
1986年 | 40篇 |
1985年 | 28篇 |
1984年 | 59篇 |
1983年 | 25篇 |
1982年 | 43篇 |
1981年 | 40篇 |
1980年 | 20篇 |
1979年 | 27篇 |
1977年 | 21篇 |
1975年 | 16篇 |
1974年 | 14篇 |
1973年 | 14篇 |
1971年 | 13篇 |
排序方式: 共有9815条查询结果,搜索用时 31 毫秒
51.
A genetically restricted suppressor factor that requires interaction with two distinct targets 总被引:6,自引:0,他引:6
I Aoki M Usui M Minami M E Dorf 《Journal of immunology (Baltimore, Md. : 1950)》1984,132(4):1735-1740
We have previously described a genetically restricted suppressor factor (TsF3) that suppresses the terminal phases of the contact sensitivity response. The activity of TsF3 is restricted by genes in the H-2 (I-J) and Igh complexes. This report analyzes the mechanisms responsible for these genetic restrictions. One cellular target of TsF3 is an I-J-bearing antigen-presenting cell population that is sensitive to low doses of cyclophosphamide. To elicit suppression I-J homology is required between this antigen-presenting cell population and the TsF3 donor. In contrast, the Igh-linked genetic restriction exists between TsF3 and an unprimed cell population present in the recipient. These findings suggest that under these experimental conditions TsF3 acts by bridging the APC with cells of the host. Finally, we demonstrated that nonspecific bystander or cognate suppression can be mediated by TsF3, provided specific antigen is present in the site of the ongoing T cell response. 相似文献
52.
H Fujiwara H Aoki T Yoshioka S Tomita R Ikegami T Hamaoka 《Journal of immunology (Baltimore, Md. : 1950)》1984,133(1):509-514
Preinduction of potent hapten-reactive helper T cell activity and subsequent immunization with hapten-coupled syngeneic tumor cells result in enhanced induction of tumor-specific immunity through T-T cell collaboration between anti-hapten helper T cells and tumor-specific effector T cells. On the basis of this augmenting mechanism, a tumor-specific immunotherapy protocol was established in which a growing tumor regresses by utilizing a potent trinitrophenyl (TNP)-helper T cell activity. C3H/He mice were allowed to generate the amplified (more potent) TNP-helper T cell activity by skin painting with trinitrochlorobenzene (TNCB) after pretreatment with cyclophosphamide. Five weeks later, the mice were inoculated intradermally with syngeneic transplantable X5563 tumor cells. When TNCB was injected into X5563 tumor mass, an appreciable number of growing tumors, in the only group of C3H/He mice in which the amplified TNP-helper T cell activity had been generated were observed to regress (regressor mice). These regressor mice were shown to have acquired tumor-specific T cell-mediated immunity. Such immunity was more potent than that acquired in mice whose tumor was simply removed by surgical resection. These results indicate that in situ TNP haptenation of the tumor cells in TNP-primed mice can induce the enhanced tumor-specific immunity leading to the regression of a growing tumor. Most importantly, the present study further investigates the applicability of this TNP immunotherapy protocol to an autochthonous tumor system. The results demonstrate that an appreciable percent of growing methylcholanthrene-induced autochthonous tumors regressed by the above TNP immunotherapy protocol. Thus, the present model provides an effective maneuver for tumor-specific immunotherapy in syngeneic transplantable as well as autochthonous tumor systems. 相似文献
53.
Dispersion capabilities of new queens were studied in the two haplometrotic paper wasps Polistes riparius and P. snelleni. New queens were marked on the nests in the late summer and located in the next spring. Dispersion distances greatly varied among queens: although a large part of recovered queens nested in close proximity to their natal sites, some did disperse over 100–300 m. This suggests that queens' emigration from and immigration into the censused areas occurred to a substantial extent. On the whole, these species exhibited a weaker “philopatric” tendency than those so far studied for dispersion distance, and seem to have the potential for a long-distance dispersion. 相似文献
54.
Summary Two new species of the Mermithidae (Nematoda) are described from the Japanese blackfly Simulium japonicum Matsumura. They are the first representatives of Gastromermis and Isomermis from Japan. Gastromermis mesostoma n. sp. is characterized by a terminal or near terminal mouth, 6 hypodermal cords and the absence of cloacal or vulvar lips.
Isomermis bipapillatus n. sp. is characterized by the small size of the spicules, their curved shape and by a pair of terminal hypodermal protuberances
in the tail.
This work was in part supported by a grant (No. 58570188) from the Japanese Ministry of Education, Science and Culture
This work was in part supported by a grant (No. 58570188) from the Japanese Ministry of Education, Science and Culture 相似文献
55.
56.
Nobuo Aoki 《Journal of Protein Chemistry》1986,5(4):269-277
Fibrinolytic system is one of the major proteolytic pathways in vivo and primarily responsible for dissolution of thrombi. Two enzymes are primarily involved in this proteolytic system; plasminogen activator (PA) and plasmin. Plasmin is formed by a limited proteolysis of plasminogen by PA, which is mainly synthesized by and secreted from vascular endothelial cells. This proteolytic process proceeds physiologically only on the surface of fibrin. Thus, initiation and progression of the fibrinolytic process depend on the function of endothelial cells and fibrin formation. Endothelial cells may also synthesize and excrete PA inhibitor (PAI) which inhibits immediately, PA once released. The rates of synthesis and excretion of PA and PAI by endothelial cells are regulated by various factors. Among them, thrombin stimulates the release of PA whereas activated protein C may decrease the release of PAI. Thus, both enzymes enhance fibrinolytic potential. PA which has escaped from inhibition by PAI binds to fibrin. 2-Plasmin inhibitor (2PI) inhibits the binding of plasminogen to fibrin, thereby suppressing this fibrin-associated plasminogen activation. A part of 2PI is cross-linked to fibrin by activated factor XIII when fibrin is formed, and the 2PI thus cross-linked to fibrin inhibits in situ plasmin formed on fibrin. Thus, 2PI as well as PAI plays a central role in inhibition of fibrinolysis. 相似文献
57.
Postmortem changes in uric acid and ascorbic acid in human cerebral cortex tissues excised after cardiac death 总被引:2,自引:0,他引:2
K Iriyama T Iwamoto M Yoshiura T Aoki 《Biochemical medicine and metabolic biology》1986,36(2):186-193
There has been no report on the determination of uric acid (UA) in human brain and heart tissues. UA and ascorbic acid (AA) in human cerebral cortex and heart tissues excised after cardiac death have been studied by reversed-phase high-performance liquid chromatography (HPLC) with electrochemical detection (ECD). It has been found that the levels of AA and UA in the human cerebral cortex tissues tend to decrease and increase, respectively, after cardiac death as a function of time between death and forensic operation. In addition, it has been found that there is no special relationship between UA levels in human heart tissues and time after cardiac death, also that the UA levels in the heart are high as compared with those in human cerebral cortex tissues. We have emphasized that the HPLC-ECD method is useful in determining UA and AA in mammalian tissues by one-time chromatography to gain a better understanding of the relationship between disease and serum urate level. 相似文献
58.
Cycling of soil carbon in the first year after a clear-felling was compared with that before the felling in a Japanese red
pine forest in Hiroshima Prefecture, west Japan. The daily mean temperature at the soil surface in summer was increased after
the felling in comparison to that before felling, and the water content of both the A0 layer and the surface mineral soil was decreased due to the loss of the forest canopy. The rate of weight loss of the A0 layer was reduced after felling. However, accumulation of the A0 layer rapidly decreased because of the lack of litter supply to the forest floor. Low soil respiration after felling was
mainly caused by the cessation of root respiration. Analysis of annual soil carbon cycling was then conducted using a compartment
model. The relative decomposition rate of the A0 layer decreased whereas that of humus and dead roots in mineral soil increased to some extent after felling. The accumulation
of carbon in mineral soil, however, increased slightly due to the supply of humus from roots killed by the felling. 相似文献
59.
Experiments were designed to examine the early events in the initiation of glutamate deamination in kidney. Perfused kidneys from methionine sulfoximine-treated rats formed ammonia from [15N]glutamate via the purine nucleotide cycle. The turnover of the 6-amino group of adenine nucleotides to yield ammonia occurred at the rate of 0.30 mumol/g of kidney/min. This rate is 3-4 times larger than in liver and is in agreement with published rates of the purine nucleotide cycle in kidney. The addition of 0.1 mM fluorocitrate to glutamate perfusions stimulated ammonia formation 3 1/2-fold. The turnover of the 6-amino group of adenine nucleotides increased during the first 5 min after adding fluorocitrate to form ammonia predominately from tissue glutamate and aspartate. This turnover correlates with a 3 1/2-fold increase in kidney tissue IMP levels. As the ATP/ADP ratio fell the purine nucleotide cycle was inhibited and glutamate dehydrogenase was stimulated to form ammonia stoichiometric with glutamate taken up from the perfusate. Ammonia formation via glutamate dehydrogenase occurred at a rate of 1.0 mumol/g of kidney/min. Fluorocitrate completely blocked ammonia formation from aspartate in perfusions. The perfused kidney formed ammonia from aspartate via the purine nucleotide cycle at a rate of 1.0 mumol/g of kidney/min. The results indicate a discrete role for aspartate in renal metabolism. Ammonia formation via the purine nucleotide cycle can occur at significant rates and equal to the rate of ammonia formation from glutamate via glutamate dehydrogenase. 相似文献
60.
Factor XIII-mediated cross-linking of NH2-terminal peptide of alpha 2-plasmin inhibitor to fibrin 总被引:1,自引:0,他引:1
The NH2-terminal 12-residue peptide of alpha 2-plasmin inhibitor, Asn-Gln-Glu-Gln-Val-Ser-Pro-Leu-Thr-Gly-Leu-Lys-NH2 . AcOH, was found to be a good substrate for plasma transglutaminase (activated blood coagulation factor XIII) and rapidly incorporated into fibrin by the enzyme. A high concentration of the peptide inhibited the enzyme-mediated cross-linking of alpha 2-plasmin inhibitor to fibrin probably by competing with the inhibitor for the same site of fibrin alpha-chain. 相似文献