Bioengineering for the industrial production of 2,3-butanediol by the yeast,Saccharomyces cerevisiae

World Journal of Microbiology and Biotechnology - Avilamycin, an excellent growth-promoting feed additive, produced by Streptomyces viridochromogenes, was widely used to promote the growth of...     

Prevalence and genomic characterization of Group A Streptococcus dysgalactiae subsp. equisimilis isolated from patients with invasive infections in Toyama prefecture,Japan

Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes severe invasive streptococcal infections, especially in elderly people. Between 2013 and 2018, 88 streptococci were isolated from clinical blood culture in a hospital in Toyama prefecture, Japan. The collection included six Group A SDSE (ASD) strains, which are rarely isolated. Multilocus sequence typing categorized five of the six strains into ST128 and the remaining strain into a new type. Maximum-likelihood phylogenetic analysis revealed that the six ASD strains had highly similar genome sequences. Bayesian analysis indicated that the most recent common ancestor of the strains appeared 39 years ago. The ASD strains possessed carbohydrate synthase genes that are conserved in Streptococcus pyogenes strains, whereas one strain featured a different arrangement of the gene cluster. The carbohydrate synthase genes varied by Lancefield type (A, C, and G).     

Designing eco-villages for revitalizing Japanese rural areas

Reestablishing a sound environment in rural areas has become one of the most important environmental issues in Japan, not only in rural policy but also in national land policy. Physically speaking, regeneration of natural ecosystems, reestablishment of sound material flows, and the design of rural landscapes are important areas of research and practice. Another important issue in the management of rural areas, particularly in the mountainous areas that cover 61% of the country, is the preparation of the landscape for urban dwellers. This paper considers methods for improving rural environments. The modeling of ideal eco-villages with sound natural environment, low input and sustainable material flow, and maintenance of villages through urban and rural interaction is considered. Three different types of typical eco-village models are designed in urban fringe areas, in typical rural areas and in remote mountainous areas. Further, a case-study for the search of reality in adopting such models is surveyed in Chosei-gun, Chiba prefecture as a case of an eco-village in a typical rural areas.