全文获取类型
收费全文 | 3654篇 |
免费 | 279篇 |
国内免费 | 3篇 |
出版年
2021年 | 29篇 |
2019年 | 24篇 |
2018年 | 31篇 |
2017年 | 29篇 |
2016年 | 57篇 |
2015年 | 93篇 |
2014年 | 106篇 |
2013年 | 188篇 |
2012年 | 201篇 |
2011年 | 176篇 |
2010年 | 91篇 |
2009年 | 88篇 |
2008年 | 170篇 |
2007年 | 181篇 |
2006年 | 140篇 |
2005年 | 148篇 |
2004年 | 141篇 |
2003年 | 133篇 |
2002年 | 156篇 |
2001年 | 130篇 |
2000年 | 135篇 |
1999年 | 108篇 |
1998年 | 56篇 |
1997年 | 50篇 |
1996年 | 33篇 |
1995年 | 36篇 |
1994年 | 34篇 |
1993年 | 43篇 |
1992年 | 77篇 |
1991年 | 75篇 |
1990年 | 69篇 |
1989年 | 87篇 |
1988年 | 55篇 |
1987年 | 61篇 |
1986年 | 56篇 |
1985年 | 71篇 |
1984年 | 67篇 |
1983年 | 38篇 |
1982年 | 39篇 |
1981年 | 31篇 |
1980年 | 19篇 |
1979年 | 45篇 |
1978年 | 31篇 |
1977年 | 37篇 |
1976年 | 23篇 |
1975年 | 26篇 |
1974年 | 26篇 |
1973年 | 26篇 |
1972年 | 24篇 |
1970年 | 23篇 |
排序方式: 共有3936条查询结果,搜索用时 15 毫秒
71.
72.
73.
74.
75.
76.
77.
78.
Ryozo Oishi Naoto Adachi Keishi Okada Nobuyuki Muroi Kiyomi Saeki 《Journal of neurochemistry》1990,55(6):1899-1904
To clarify the regulation of central histaminergic (HAergic) activity by cholinergic receptors, the effects of drugs that stimulate the cholinergic system on brain histamine (HA) turnover were examined, in vivo, in mice and rats. The HA turnover was estimated from the accumulation of tele-methylhistamine (t-MH) during the 90-min period after administration of pargyline (65 mg/kg, i.p.). In the whole brain of mice, oxotremorine, at doses higher than 0.05 mg/kg, s.c., significantly inhibited the HA turnover, this effect being completely antagonized by atropine but not by methylatropine. A large dose of nicotine (10 mg/kg, s.c.) also significantly inhibited the HA turnover. This inhibitory effect was antagonized by mecamylamine but not by atropine or hexamethonium. A cholinesterase inhibitor, physostigmine, at doses higher than 0.1 mg/kg, s.c., significantly inhibited the HA turnover. This effect was antagonized by atropine but not at all by mecamylamine. None of these cholinergic antagonists used affected the steady-state t-MH level or HA turnover by themselves. In the rat brain, physostigmine (0.1 and 0.3 mg/kg, s.c.) also decreased the HA turnover. This inhibitory effect of physostigmine was especially marked in the striatum and cerebral cortex where muscarinic receptors are present in high density. Oxotremorine (0.2 mg/kg, s.c.) and nicotine (1 mg/kg, s.c.) also decreased the HA turnover in the rat brain. However, these effects showed no marked regional differences. These results suggest that the stimulation of central muscarinic receptors potently inhibits the HAergic activity in the brain and that strong stimulation of central nicotinic receptors can also induce a similar effect. 相似文献
79.
80.
Purification and characterization of membrane-bound inositolpolyphosphate 5-phosphatase 总被引:1,自引:0,他引:1
Membrane-bound inositolpolyphosphate 5-phosphatase was solubilized and highly purified from a microsomal fraction of rat liver. Its physiochemical and enzymological properties were compared with those of highly purified preparations of two types of soluble enzyme (soluble Type I and Type II) from rat brain. The molecular masses of the membrane-bound and soluble Type I enzymes were 32 kDa, while that of soluble Type II enzyme was 69 kDa, as determined by molecular sieve chromatography. The membrane-bound and soluble Type I enzymes showed similar broad peaks on isoelectric focusing (pI 5.8-6.4), while soluble Type II enzyme showed multiple peaks in the region between pI 4.0-5.8. All three enzymes required divalent cation for activity. Mg2+ was the most effective for both the membrane-bound and soluble Type I enzymes, while Co2+ enhanced soluble Type II enzyme activity about 1.5-fold relative to Mg2+ at 1 mM. The optimal pH of both the membrane-bound and soluble Type I enzymes was 7.8, while that of soluble Type II was 6.8. The Km values for inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] of all three enzymes were similar (5-8 microM), but those for inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4] were quite different, the Km values of membrane-bound and soluble Type I enzymes being 0.8 microM, while that of soluble Type II was 130 microM. These similarities between the membrane-bound and soluble Type I enzymes suggest that these two molecules may be the same protein, and that concentrations of Ins(1,4,5)P3 and Ins(1,3,4,5)P4, both of which are considered to play critical roles in the regulation of intracellular Ca2+-concentration, may be differently regulated by two functionally distinct enzymes. 相似文献