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151.
Keitaro?TanoiEmail author Natsuko?I.?Kobayashi Takayuki?Saito Naoko?Iwata Risa?Kamada Ren?Iwata Hisashi?Suzuki Atsushi?Hirose Yoshimi?Ohmae Ryohei?Sugita Tomoko?M.?Nakanishi 《Plant and Soil》2014,384(1-2):69-77
Aims
The mechanisms underlying magnesium (Mg) uptake by plant roots remain to be fully elucidated. In particular, there is little information about the effects of Mg deficiency on Mg uptake activity. A Mg uptake kinetic study is essential for better understanding the Mg uptake system.Methods
We performed a Mg uptake tracer experiment in rice plants using 28?Mg.Results
Mg uptake was mediated by high- and low-affinity transport systems. The K m value of the high-affinity transport system was approximately 70 μM under Mg-deficient conditions. The Mg uptake activity was promoted by Mg deficiency, which in turn fell to the basal level after 5- min of Mg resupply. The induced uptake rate was inhibited by ionophore treatment, suggesting that an energy-dependent uptake system is enhanced by Mg deficiency.Conclusions
The Mg uptake changes rapidly with Mg conditions in rice, as revealed by a 28?Mg tracer experiment. This technique is expected to be applicable for Mg uptake analyses, particularly in mutants or other lines.152.
153.
K Nakanishi K Nara H Hagiwara Y Aoyama H Ueno S Hirose 《European journal of biochemistry》1991,202(1):15-21
A cDNA clone encoding transglutaminase was isolated from a bovine-endothelial-cell cDNA library using oligonucleotide probes designed based on partial amino acid sequences of the purified protein. Sequencing of the cDNA insert revealed an open reading frame of 2061 bp coding for a protein of 687 amino acids. The sequence of bovine endothelial-cell transglutaminase was 88, 82, 80, 37, 37 and 37% identical with that of human endothelial, rat macrophage, guinea-pig liver, human and rat keratinocyte transglutaminases, and the human blood-coagulation factor XIIIa subunit, respectively. The cDNA clone was hybridized to a single mRNA species of 3.9 kb in the liver, lung, spleen and heart but not hybridized to RNA from the brain. Northern-blot analysis of mRNA from retinoid-treated cultured vascular endothelial cells revealed that retinoids were able to induce a large increase in the transglutaminase mRNA levels. 相似文献
154.
Yasuhiro Kidera Hisato Kawakami Tsutomu Sakiyama Kunio Okamoto Kaoru Tanaka Masayuki Takeda Hiroyasu Kaneda Shin-ichi Nishina Junji Tsurutani Kimiko Fujiwara Morihiro Nomura Yuzuru Yamazoe Yasutaka Chiba Shozo Nishida Takao Tamura Kazuhiko Nakagawa 《PloS one》2014,9(7)
Background
Nephrotoxicity remains a problem for patients who receive cisplatin chemotherapy. We retrospectively evaluated potential risk factors for cisplatin-induced nephrotoxicity as well as the potential impact of intravenous magnesium supplementation on such toxicity.Patients and Methods
We reviewed clinical data for 401 patients who underwent chemotherapy including a high dose (≥60 mg/m2) of cisplatin in the first-line setting. Nephrotoxicity was defined as an increase in the serum creatinine concentration of at least grade 2 during the first course of cisplatin chemotherapy, as assessed on the basis of National Cancer Institute Common Terminology Criteria for Adverse Events version 4.0. The severity of nephrotoxicity was evaluated on the basis of the mean change in the serum creatinine level. Magnesium was administered intravenously to 67 patients (17%).Results
Cisplatin-induced nephrotoxicity was observed in 127 patients (32%). Multivariable analysis revealed that an Eastern Cooperative Oncology Group performance status of 2 (risk ratio, 1.876; P = 0.004) and the regular use of nonsteroidal anti-inflammatory drugs (NSAIDs) (risk ratio, 1.357; P = 0.047) were significantly associated with an increased risk for cisplatin nephrotoxicity, whereas intravenous magnesium supplementation was associated with a significantly reduced risk for such toxicity (risk ratio, 0.175; P = 0.0004). The development of hypomagnesemia during cisplatin treatment was significantly associated with a greater increase in serum creatinine level (P = 0.0025). Magnesium supplementation therapy was also associated with a significantly reduced severity of renal toxicity (P = 0.012).Conclusions
A relatively poor performance status and the regular use of NSAIDs were significantly associated with cisplatin-induced nephrotoxicity, although the latter association was marginal. Our findings also suggest that the ability of magnesium supplementation to protect against the renal toxicity of cisplatin warrants further investigation in a prospective trial. 相似文献155.
Photosymbiotic ascidian fauna were surveyed in the subtidal zone off Pari Island in the Thousand Islands (Java Sea, Indonesia). Nine species were recorded: Didemnum molle, Trididemnum miniatum, Lissoclinum patella, L. punctatum, L. timorense, Diplosoma gumavirens, D. simile, D. simileguwa, and D. virens. All of these species have been previously recorded in the Ryukyu Archipelago, Japan. Diplosoma gumavirens and D. simileguwa were originally described from the Ryukyu Archipelago in 2009 and 2005, respectively, and all of the observed species are potentially widely distributed in Indo–West Pacific coral reefs. 相似文献
156.
157.
Suzuki Hajime Sakabe Takahiro Hirose Yuu Eki Toshihiko 《Applied microbiology and biotechnology》2017,101(2):659-671
Applied Microbiology and Biotechnology - We aimed to develop the bioassays for genotixicity and/or oxidative damage using the recombinant yeast. A genotoxicity assay was developed using recombinant... 相似文献
158.
Masaaki Hirose Etsuro Sugimoto Hideo Chiba 《Bioscience, biotechnology, and biochemistry》2013,77(12):2157-2162
It was found that fructose 1,6-diphosphate, the main intermediate of glycolysis, was able to act as a coenzyme of yeast phosphoglucomutase reaction. The mechanism of the coenzymatic activity of fructose 1,6-diphosphate was studied. It was indicated in the fructose 1,6-diphosphate dependent reaction that glucose 1,6-diphosphate was formed by the phosphate-transfer of fructose 1,6-diphosphate to glucose 1-phosphate in the first step, and in the second step the conversion of glucose 1-phosphate to glucose 6-phosphate, the original mutase reaction, occurred in the presence of glucose 1,6-diphosphate. The kinetic constants in the reaction of the first step were determined from the time courses of the fructose 1,6-diphosphate dependent reaction. 相似文献
159.
Ayaaki Ishizaki Hiroshiro Shibai Yoshio Hirose Teruo Shiro 《Bioscience, biotechnology, and biochemistry》2013,77(1):107-113
In inosine fermentation, the yield of the product was closely related to the partial pressure of carbon dioxide in the culture system rather than the bicarbonate ion concentration in the culture liquid. The inhibitory effect of carbon dioxide was restored by the methods of reducing the partial pressure of carbon dioxide lower than a certain level. Both ventilation and chemical absorption were applicable for the restoration of the carbon dioxide inhibition, but ventilation had great advantages over the other method from an industrial view-point. In the estimation of aeration-agitation conditions for the scale-up of submerged fermentation in which carbon dioxide was inhibitory, the rate of aeration was to be established to make sufficient ventilation and overcome this inhibition. A scheme for the procedure for the estimation of aeration-agitation conditions under the consideration of influences of carbon dioxide on submerged fermentation was proposed in this paper. 相似文献
160.
MAP kinase cascades in elicitor signal transduction 总被引:3,自引:0,他引:3
Suzuki K 《Journal of plant research》2002,115(3):0237-0244
Protein kinases play important roles in elicitor signal transduction. In this article, I describe the current view of the
role of mitogen-activated protein kinase (MAPK) cascades in elicitor signal transduction of plant cells based on our own research
and recent developments in this field. In the past several years, it has become apparent that MAPK cascades play important
roles in elicitor signal transduction in plants. Our early studies demonstrated the identification of p47 MAPK in tobacco
as an elicitor-responsive protein kinase and possible involvement of p47 MAPK in elicitor signal transduction to induce defense
responses, including defense gene expression and hypersensitive cell death. However, the molecular identity of p47 MAPK is
still unclear. Recent important studies suggest that tobacco MAPK cascades that include SIPK, and/or WIPK, and NtMEK2, an
upstream kinase for both SIPK and WIPK, have a crucial function in induction of defense responses and hypersensitive cell
death. The orthologs of these protein kinases in Arabidopsis and alfalfa are also suggested to have similar functions. Furthermore, the identification of loss-of-function mutation in
Arabidopsis reveals a negative regulatory role for putative MAPK cascades in plant defense mechanisms.
Received: February 7, 2002 / Accepted: February 25, 2002 相似文献