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41.
Generation of factor VIII cofactor activity requires divalent metal ions such as Ca2+ or Mn2+. Evaluation of cofactor reconstitution from isolated factor VIIIa subunits revealed the presence of a functional Ca2+ binding site within the A1 subunit. Isothermal titration calorimetry demonstrated at least two Ca2+ binding sites of similar affinity (K(d) = 0.74 microm) within the A1 subunit. Mutagenesis of an acidic residue-rich region in the A1 domain (residues 110-126) homologous to a putative Ca2+ binding site in factor V (Zeibdawi, A. R., and Pryzdial, E. L. (2001) J. Biol. Chem. 276, 19929-19936) and expression of B-domainless factor VIII molecules yielded reagents to probe Ca2+ and Mn2+ binding in a functional assay. Basal activity observed for wild type factor VIII in a metal ion-free buffer was enhanced approximately 2-fold with saturating Ca2+ or Mn2+ and yielded functional K(d) values of 1.2 and 1.40 microm, respectively. Ca2+ binding affinity was greatly reduced (or lost) in several mutants including E110A, E110D, D116A, E122A, D125A, and D126A. Alternatively, E113A, D115A, and E124A showed wild type-like activity with little or no reduction in Ca2+ affinity. However, Mn2+ affinity was minimally altered except for mutant D125A (and D116A). These results are consistent with region 110-126 serving a critical role for Ca2+ coordination with selected residues capable of contributing to a partially overlapping site for Mn2+, and that occupancy of either site is required for maximal cofactor activity. 相似文献
42.
As reported in a previous article [Kataoka (1988a) Plant CellPhysiol. 29: 1323], growing apices of the xanthophycean coenocyticalga, Vaucheria terrestris, bends away from a unilateral bluelight (BL) source, if they are simultaneously irradiated withstrong background BL in a solution containing 14 mM Ca2+.Since the negative bending is a function of the product of theexternal Ca2+ concentration and the fluence rate of backgroundBL, a BL-induced Ca2+-influx at the apex was hypothesized tobe the cause of the phototropic inversion. The present reportprovides strong evidence for this hypothesis. Addition of theCa2+ channel blockers, La3+, verapamil, nifedipine and nitrendipineto media containing 4 mM Ca2+ completely inhibited the phototropicinversion. By contrast, 1 µM A23187
[GenBank] (plus 4 mM Ca2+) notonly enhanced the phototropic inversion under background BL,but also mimicked the background BL; i.e. it caused negativebending under safe red light. Inhibition of phototropic inversionby La3+ is also observed under conditions where the algae areirradiated with unilateral BL for 1 week. A BL-dependent Ca2+influx and a consequential elevation of the cytoplasmic Ca2+-levelin the apical growth region must be involved in the early stepsof phototropic response. A BL-controlled opening of L-type Ca2+channels is also suggested.
1A part of this study was reported at the 3rd Phycological Congressat Melbourne 1988 (Kataoka 1988b) and XXII Yamada Conferenceon Plant Water Relations and Growth Under Stress at Osaka 1989(Kataoka 1989)
2Dedicated to Prof. em. Dr. Noburo Kamiya on the occasion ofhis 77th birthday (Received May 23, 1990; Accepted July 13, 1990) 相似文献
43.
In the presence of 18 mM Ca2+ unilateral 1015min irradiation with blue light can elicit negative phototropicbending in the tip-growing coenocytic fresh water alga, Vaucheriaterrestris (Xanthophyceae), when it is simultaneously irradiatedwith strong blue or green background light. By changing wavelength,fluence rate, and duration of background light and holding thoseof unilateral light (456 nm, 1.7 Wm2) negative phototropicresponse was analyzed: the wavelength of the background lighthad to be shorter than 540 nm; red light (660 nm) was ineffectiveeven at very high fluence rates (100W.m2). The negativebending was strongly and specifically dependent on the externalCa2+ concentration. Other divalent cations, Mg2+ and Ba2+, wereeither toxic or quite ineffective; Sr2+ could partly supportthe growth, but mediated neither positive nor negative phototropicbending. The rate of tip-growth was not significantly alteredbetween 106M and 10-6 mM Ca2+. Pre-irrradiation with the backgroundlight slightly increased the negative curvature; whereas thatwithout subsequent simultaneous irradiation does not cause negativebending, but rather increases the positive curvature. Three-mindelayed start of background light did not cause negative bendingany more. The present results strongly suggest that blue light elicitsan influx of Ca2+ at the apex of Vaucheria and that the increasedcytoplasmic Ca2+ regulates the sensitivity and direction ofphototropic response. (Received June 23, 1988; Accepted September 7, 1988) 相似文献
44.
The salinity tolerance ofVaucheria dichotoma, a siphonous Xanthophycean alga was investigated. The alga survived an external osmotic potential range between 74 and 1,
176 mOsmol (ca. 2.5 and 40.0 ppt. (parts per thousand]). Turgor pressure was regulated in salinities ranging from 74 to 441
mOsmol. With further increase of the salinity, turgor pressure decreased from 153 to 9 mOsmol (0.44 to 0.08 MPa). At 441 mOsmol
salinity the major intracellular ions were present in the following concentrations (mM/l cell water): K+, 145; Na+; 90; sulphate, 91; Cl−, 91. Under the most severe salinity stress (1,176 mOsmol) the ionic concentration increased to (mM/l cell water): K+, 250; Na+, 75; sulphate, 35; Cl−, 351. The content of amino acids: alanine (Ala), threonine (Thr and glutamic acid (Glu) was lower, nerver exceeding 5–11
mM, however; the concentrations were positively correlated with salinity. 相似文献
45.
Wakabayashi H Zhou Q Nogami K Ansong C Varfaj F Miles S Fay PJ 《Biochimica et biophysica acta》2006,1764(6):1094-1101
Reconstitution of factor VIII from isolated heavy chain (HC) and light chain (LC) shows pH-dependence. In the presence of Ca2+, up to 80% of native factor VIII activity was recovered over a wide range of pH. In contrast, affinity of HC and LC was maximal at pH 6.5-6.75 (Kd approximately 4 nM), whereas a Kd approximately 20 nM was observed at physiological pH (7.25). The effect of Cu2+ (0.5 microM total Cu2+) on maximal activity regenerated was negligible at pH 6.25-8.0. However, this level of Cu2+ increased the inter-chain affinity by approximately 5-fold at pH 7.25. This effect resulted from an approximately 1.5-fold increased association rate constant (k(on)) and an approximately 3-fold reduced dissociation rate constant (k(off)). High affinity (Kd=5.3 fM) of the factor VIII heterodimer for Cu2+ was estimated by increases in cofactor activity. No significant increase in inter-chain affinity was observed when either isolated chain was reacted with Cu2+ followed by addition of the complementary chain. Together, these results suggest that the protonation state of specific residues modulates inter-chain affinity. Furthermore, copper ion contributes to the maintenance of the heterodimer at physiologic pH by a mechanism consistent with bridging the two chains. 相似文献
46.
A chemical synthesis of uridine 5’-diphospho-N-acetyllactosamine (Galβ(1→4)GlcNAc-UDP; UDP-LacNAc) and Galβ(1→3)GlcNAc-UDP is described. Coupling of the disaccharide imidate
derivatives with dibenzylphosphate gave the corresponding 1-phosphates, which were condensed with UMP-imidazolate to give
the target UDP-oligosaccharides after purification by anion exchange HPLC and gel filtration column chromatography. Using
this methodology a variety of oligosaccharide nucleotide analogues can be synthesized. These UDP-oligosaccharides may be useful
for finding so-called `oligosaccharide transferases’, the glycosyltransferases which transfer the oligosaccharide moiety onto
glycosyl acceptors. 相似文献
47.
Tada T Suzuki K Sakurai Y Kubo M Okada H Itoh T Tsukamoto K 《Journal of virology》2011,85(4):1834-1846
The molecular basis of pathogenicity of H5N1 highly pathogenic avian influenza (HPAI) viruses in chickens remains largely unknown. H5N1 A/chicken/Yamaguchi/7/2004 virus (CkYM7) replicates rapidly in macrophages and vascular endothelial cells in chickens, causing sudden death without fever or gross lesions, while H5N1 A/duck/Yokohama/aq10/2003 virus (DkYK10) induces high fever, severe gross lesions, and a prolonged time to death, despite the 98% amino acid identity between the two viruses. To explore the molecular basis of this difference in pathogenicity, a series of eight single-gene reassortant viruses from these HPAI viruses were compared for pathogenicity in chickens. Two reassortants possessing the NP or PB2 gene from DkYK10 in the CkYM7 background reduced pathogenicity compared to other reassortants or CkYM7. Inversely, reassortants possessing the NP or PB2 gene of CkYM7 in the DkYK10 background (rgDkYK-PB2(Ck), rgDkYK-NP(Ck)) replicated quickly and reached higher titers than DkYK10, accompanied by more rapid and frequent apoptosis of macrophages. The rgDkYK-NP(Ck) and rgDkYK-PB2(Ck) reassortants also replicated more rapidly in chicken embryo fibroblasts (CEFs) than did rgDkYK10, but replication of these viruses was similar to that of CkYM7 and DkYK10 in duck embryo fibroblasts. A comparison of pathogenicities of seven rgDkYK10 mutants with a single amino acid substitution in NP(Dk) demonstrated that valine at position 105 in the NP(Ck) was responsible for the increased pathogenicity in chickens. NP(Ck), NP(105V), and PB2(Ck) enhanced the polymerase activity of DkYK10 in CEFs. These results indicate that both NP and PB2 contribute to the high pathogenicity of the H5N1 HPAI viruses in chickens, and valine at position 105 of NP may be one of the determinants for adaptation of avian influenza viruses from ducks to chickens. 相似文献
48.
Osanai M Saegusa H Kazuno AA Nagayama S Hu Q Zong S Murakoshi T Tanabe T 《Biochemical and biophysical research communications》2006,344(3):920-925
Voltage-dependent Ca(2+) channels play important roles in cerebellar functions including motor coordination and learning. Since abundant expression of Ca(V)2.3 Ca(2+) channel gene in the cerebellum was detected, we searched for possible deficits in the cerebellar functions in the Ca(V)2.3 mutant mice. Behavioral analysis detected in delayed motor learning in rotarod tests in mice heterozygous and homozygous for the Ca(V)2.3 gene disruption (Ca(V)2.3+/- and Ca(V)2.3-/-, respectively). Electrophysiological analysis of mutant mice revealed perplexing results: deficit in long-term depression (LTD) at the parallel fiber Purkinje cell synapse in Ca(V)2.3+/- mice but apparently normal LTD in Ca(V)2.3-/- mice. On the other hand, the number of spikes evoked by current injection in Purkinje cells under the current-clamp mode decreased in Ca(V)2.3 mutant mice in a gene dosage-dependent manner, suggesting that Ca(V)2.3 channel contributed to spike generation in Purkinje cells. Thus, Ca(V)2.3 channel seems to play some roles in cerebellar functions. 相似文献
49.
Zoey Tay Ru Jun Eng Kenichi Sajiki Kim Kiat Lim Ming Yi Tang Mitsuhiro Yanagida Ee Sin Chen 《PloS one》2013,8(1)
Doxorubicin is an anthracycline antibiotic that is among one of the most commonly used chemotherapeutic agents in the clinical setting. The usage of doxorubicin is faced with many problems including severe side effects and chemoresistance. To overcome these challenges, it is important to gain an understanding of the underlying molecular mechanisms with regards to the mode of action of doxorubicin. To facilitate this aim, we identified the genes that are required for doxorubicin resistance in the fission yeast Schizosaccharomyces pombe. We further demonstrated interplay between factors controlling various aspects of chromosome metabolism, mitochondrial respiration and membrane transport. In the nucleus we observed that the subunits of the Ino80, RSC, and SAGA complexes function in the similar epistatic group that shares significant overlap with the homologous recombination genes. However, these factors generally act in synergistic manner with the chromosome segregation regulator DASH complex proteins, possibly forming two major arms for regulating doxorubicin resistance in the nucleus. Simultaneous disruption of genes function in membrane efflux transport or the mitochondrial respiratory chain integrity in the mutants defective in either Ino80 or HR function resulted in cumulative upregulation of drug-specific growth defects, suggesting a rewiring of pathways that synergize only when the cells is exposed to the cytotoxic stress. Taken together, our work not only identified factors that are required for survival of the cells in the presence of doxorubicin but has further demonstrated that an extensive molecular crosstalk exists between these factors to robustly confer doxorubicin resistance. 相似文献
50.
5-Bromouracil derivatives (1) reacted with carbanions at room temperature to give 6-substituted uracils (6), 2,4-diazabicyclo[4,1,0] heptanes (8) and 2,4-diazabicyclo[4,3,0]nonane derivative (9), which depend largely upon the structure of the active methylene compounds employed for the generation of carbanions. 相似文献