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991.
992.
Cryosurgery was used as a mode of treatment in 31 patients with advanced tumours.The patients were selected because of failure of other forms of conventional treatment or because a less radical type of therapy was advisable.The primary aim of the treatment was palliation. Pain was well controlled in almost all patients in whom the main bulk of the tumour could be successfully destroyed. Bleeding was also controlled temporarily and obstruction prevented in other patients.Cryosurgery is a simple procedure, easy to perform, has very few complications and can be repeated at frequent intervals. It is well tolerated by the elderly patients and produces very little postoperative discomfort, permitting earlier discharge from hospital.In selected cases of cancer, cryosurgery has a definite role to play in improving the quality of survival. It is particularly useful in extensive and recurrent cancers of the head and neck region. 相似文献
993.
Mesophyll and bundle sheath chloroplasts were prepared fromleaves of Zea mays grown at light intensities of 1.1 and 240µW/cm2, respectively. The mesophyll chloroplasts thatdeveloped at the low intensity and bundle sheath chloroplatsthat developed at both low and high intensities showed higherratios of chlorophyll a/b and P700/chlorophylls compared withthe normal ratios found for the mesophyll chloroplasts thathad developed at the high intensity. Derivative absorption spectrophotometryat 77?K revealed that the low intensity mesophyll chloroplastscontained more of chlorophyll a forms with longer wavelengthred bands than high intensity mesophyll chloroplasts. More ofthe longer wavelength forms of chlorophyll a were also presentin the bundle sheath chloroplasts that had developed at lowand high intensities. All these four types of chloroplasts showedtwo peaks of fluorescence, one at 687 hra and the other at 733or 738 nm. In addition to these peaks, the high intensity mesophyllchloroplasts showed a shoulder at 697 nm, and the two typesof bundle sheath chloroplasts showed a shoulder at 680 nm. (Received June 17, 1974; ) 相似文献
994.
The effect of dihydroconiferyl alcohol (DCA) on elongation ofcucumber hypocotyl segments was studied. DCA did not influenceelongation when given alone, but synergistically enhanced IAA-inducedelongation. The stimulating effect of DCA was conspicuous particularlywhen it was given together with suboptimal concentrations ofIAA. DCA hardly influenced GA-induced elongation in the presenceof 2% sucrose. (Received May 22, 1974; ) 相似文献
995.
996.
997.
Effects of Mn++ on 45Ca content and potassium-induced contraction of the aortic strip 总被引:3,自引:0,他引:3
S Shibata 《Canadian journal of physiology and pharmacology》1969,47(9):827-829
998.
Intrinsic DNA-dependent ATPase activity of reverse gyrase 总被引:4,自引:0,他引:4
Reverse gyrase is a type I DNA topoisomerase that promotes positive supercoiling of closed-circular double-stranded DNA through an ATP-dependent reaction, and it was purified from an archaebacterium, Sulfolobus. When ATP is replaced by UTP, GTP, or CTP, this enzyme just relaxes the negatively supercoiled closed-circular double-stranded DNA. We found that reverse gyrase hydrolyzes ATP through a double-stranded DNA-dependent reaction. The superhelicity of the DNA did not affect the ATPase activity. However, reverse gyrase does not hydrolyze UTP, GTP, or CTP. Therefore, any of the four nucleotide 5'-triphosphates acts as an effector for the topoisomerase activity of reverse gyrase, but only ATP supports the positive supercoiling of closed-circular double-stranded DNA, through the energy released on its hydrolysis. Single-stranded DNA was a much more potent cofactor for the ATPase activity of the enzyme than double-stranded DNA, and it acted as a potent inhibitor for the topoisomerase activity on double-stranded DNA. These results indicate that reverse gyrase has higher affinity to single-stranded DNA than to double-stranded DNA, which suggests a cellular function of the enzyme. 相似文献
999.
1000.
Purification of caldesmon and myosin light chain (MLC) kinase from arterial smooth muscle: comparisons with gizzard caldesmon and MLC kinase 总被引:2,自引:0,他引:2
We have developed a simple and conventional purification method for caldesmon and MLC kinase from bovine arterial smooth muscle, and compared the arterial and gizzard proteins. Arterial caldesmon shares the alternative binding to calmodulin or F-actin in a Ca2+-dependent manner and the antigenic determinants with the gizzard protein. Both caldesmons have the same association constant with F-actin (1.3-1.7 X 10(7) M-1) and the same maximum binding (1 caldesmon per 12-14 actins). However, the molecular weight of arterial caldesmon (dimer of a 148 kDa polypeptides) was slightly different from that of gizzard caldesmon (heterodimer of 150/147 kDa polypeptides). The molecular weight of arterial MLC kinase (160 kDa) was much larger than that of the gizzard enzyme (135 kDa). The enzyme activities of both MLC kinases were comparable (Km = 9.5 microM, Vmax = 12.5 mumol/min X mg). The association constant of the arterial enzyme to F-actin (5.1 X 10(6) M-1) was much larger than that of the gizzard enzyme (9.0 X 10(5) M-1) but the maximum binding was the same (1 enzyme per 12-13 actins). Immunocytochemical examinations showed that caldesmon and MLC kinase in cultured arterial cells have a restricted localization along the stress fibers, suggesting functional linkages between both proteins and actin filaments in vivo. 相似文献