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81.
82.
Histological Observations on Initiation and Morphogenesis in Immature and Mature Embryo Derived Callus of Barley (Hordeum vulgare L.) 总被引:1,自引:0,他引:1
Callus was induced from immature and mature embryos of barley(cv. Haruna Nijo) on Murashige and Skoog medium containing 2mg l-1 2,4-D and 5 mg l-1 picloram, respectively. Paraffin sections(10 µm thick) were prepared for histology during callusinitiation and plant regeneration. Meristems were regeneratedfrom nodular compact callus (NC) derived from scutellar epidermisin immature embryos, whereas they were regenerated from NC derivedfrom epidermal cells of leaf or coleoptile bases in mature embryos.Regardless of the explant source, regeneration was predominantlythrough organogenesis, although regeneration through somaticembryogenesis infrequently occurred. Thus, the callus inducedfrom immature and mature embryos of barley was regarded as 'nodularcompact' rather than 'embryogenic'.Copyright 1995, 1999 AcademicPress Barley, callus, Hordeum vulgare, histology, immature embryo, mature embryo, regeneration 相似文献
83.
The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against solamargine was produced by fusing splenocytes immunized with a solamargine-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Extensive cross-reaction of anti-solamargine antibodies against solasonine appeared. Aglycone of solamargine, solasodine cross-reacted with anti-solamargine antibodies resulting in a 43.8% cross-reaction. Insignificant cross-reaction appeared with tomatine (2.06%). The full measuring range of the assay extends from 57.5 pmol ml–1 to 11.5 nmol ml–1 of solamargine. 相似文献
84.
Strong adjuvant action of Klebsiella O3 lipopolysaccharide and its inhibition of systemic anaphylaxis 总被引:1,自引:0,他引:1
T. Yokochi Y. Inoue Y. Kato T. Sugiyama G.-Z. Jiang M. Kawai M. Fukada K. Takahashi 《FEMS immunology and medical microbiology》1995,10(3-4):181-184
Abstract Immunization with lipopolysaccharide from Klebsiella O3 as an immunological adjuvant did not cause the death of mice in systemic anaphylaxis to bovine serum albumin. On the other hand, most mice immunized with lipopolysaccharide from Escherichia coli O111, Klebsiella O4 and Salmonella minnesota did die. Klebsiella O3 lipopolysaccharide enhanced IgM and IgG antibody response to BSA more markedly than Escherichia coli O111 lipopolysaccharide, while it affected the production of IgE antibody only slightly. Therefore, it is suggested that the inhibition of systemic anaphylaxis by Klebsiella O3 lipopolysaccharide adjuvant might be related to its strong adjuvant action on IgM and IgG class antibody production, and that high levels of circulating IgM and IgG antibodies might act as blocking antibodies in the development of IgE-mediated systemic anaphylaxis. 相似文献
85.
Yukimaru Sugiyama 《Primates; journal of primatology》1984,25(4):423-432
Conspecific infanticides by male langurs depresses population growth in their densely populated habitats. An infanticidal
model developed from demographic parameters obtained in the Dharwar study area predicts an almost stable population. On the
other hand, a non-infanticidal model predicts a population growth by 2.6% per year. The effect of frequent troop usurpation
and infanticide on the control of population growth must be strong if the natality rate is high. For subadult and juvenile
surplus males, it must be difficult to survive and to mature in the all-male party in its poor habitat. However, from calculations
for living adult males and the number of troops at Dharwar, most adult males are thought to be able to obtain a troop within
five years of first challenge of usurpation. Dominant males do not always take over a troop containing more females than do
subordinate males. These simple assessments require further intensive field studies to determine the precise differences between
infanticidal and non-infanticidal populations and whether or not dominant males make a greater genetic contribution than subordinate
males to subsequent generations. 相似文献
86.
Sato Takahide; Kusaba Sakiku; Nakagawa Hiroki; Ogura Nagao 《Plant & cell physiology》1984,25(6):1069-1071
By using a monospecific anti-polygalacturonase-2 antibody, a54K-dalton polypeptide was detected in in vitro translationproducts by a wheat germ cell-free translation system programmedwith polyadenylated RNA from ripe tomato pericarp tissue. Thisputative precursor of polygalacturonase was about 9K daltonslarger in molecular weight than polygalacturonase-2. (Received December 12, 1983; Accepted May 17, 1984) 相似文献
87.
Minoru Niimoto Takao Hattori Ichiji Ito Ryuichiro Tamada Kiyoshi Inokuchi Kunzo Orita Hisashi Furue Nobuya Ogawa Tomohiro Toda Motonosuke Furusawa Shigemasa Koga Isamu Hashimoto Tatsuhei Kondo Shigeru Fujimoto Yuzuru Sugiyama Osahiko Abe Masaaki Oya 《Cancer immunology, immunotherapy : CII》1984,18(1):13-18
Summary The usefulness of LMS in postoperative immunochemotherapy of gastric cancer was investigated. In compliance with the protocol, MMC was given at a dose of 20 mg on the day of gastrectomy, and an additional 10 mg on the next day IV. The patients receiving 600 mg Tegafur daily were then divided into two groups according to whether LMS was also given or not. LMS was administered for 3 days before the operation in a daily dose of 150 mg and for 1 year or more after operation according to a schedule of 3 days' administration followed by an 11-day interval. The 2-year follow-up demonstrated that in stage III patients, the LMS (+) regimen was superior to the LMS (–) regimen, since the former prolonged the relapse-free interval significantly. The survival rate for stage III disease was also significantly higher in the LMS (+) than in the LMS (–) group. There was no significant difference in the incidence of subjective or objective side-effects between two groups. The incidence of agranulocytosis was comparable in the two groups.Gastrointestinal Cancer Research Group, Japan Levamisole Research AssociationChairmen of the Gastrointestinal Cancer Research Group, Japan LMS Research AssociationController of the Gastrointestinal Cancer Research Group, Japan LMS Research AssociationMembers of the Data Collection and Analysis SubcommitteeThis study was carried out by the Gastrointestinal Cancer Research Group, Japan LMS Research Association (directed by Prof. Kiyoshi Inokuchi, Dept. of Surgery, Kyushu University and Prof. Eiro Tsubura, Dept. of Internal Medicine, Tokushima University). The results were presented in part at the 19th General Meeting of the Japanese Society for Gastroenterological Surgery in February, 1982 相似文献
88.
89.
Culture fluids of 4 type A, 6 type B, and 1 type EClostridium botulinum strains were compared for their toxicities in 9–11-day-old (infant) and adult mice. In intraperitoneal challenges, most toxins were about equally potent for infants and adults; the exceptions were 4 type B preparations, which were 500 to 5,000 times more lethal for infants. In orogastric challenges, samples were 10 to >15,000 times more toxic for infants, and the previously mentioned type B toxins constituted a group having the highest relative toxicity for infants. 相似文献
90.
The liberation of 2,4-dinitrophenolate ion from 2,4-dinitrophenyl sulfate (DNPS) in aqueous organic solvent with 0.1 N sodium hydroxide was accelerated upon addition of an equimolar amount of Oxime-I (10-hydroxy-11-hydroxyimino[20]-paracyclophane) to the sulfate ester. Oxime-I was found to undergo covalent participation at the oxime group to afford oxime O-sulfonate. The rate acceleration with Oxime-I was larger than that with β-CD (cycloheptaamylose). The catalytic efficiency of Oxime-I has been ascribed primarily to the tighter inclusion of the substrate ester into the more hydrophobic Oxime-I cavity provided by the effective apolar paracyclophane skeleton, as well as to the greater nucleophilicity of the oxime group than of the hydroxyl group in β-CD. Consequently, Oxime-I may be considered as a conventional model for arylsulfatases and sulfotransferases, providing the effective binding process for the substrate. 相似文献