全文获取类型
收费全文 | 1595篇 |
免费 | 98篇 |
专业分类
1693篇 |
出版年
2022年 | 11篇 |
2021年 | 11篇 |
2020年 | 6篇 |
2019年 | 9篇 |
2018年 | 15篇 |
2017年 | 8篇 |
2016年 | 26篇 |
2015年 | 39篇 |
2014年 | 32篇 |
2013年 | 70篇 |
2012年 | 87篇 |
2011年 | 87篇 |
2010年 | 70篇 |
2009年 | 69篇 |
2008年 | 79篇 |
2007年 | 84篇 |
2006年 | 82篇 |
2005年 | 83篇 |
2004年 | 87篇 |
2003年 | 87篇 |
2002年 | 72篇 |
2001年 | 46篇 |
2000年 | 62篇 |
1999年 | 43篇 |
1998年 | 20篇 |
1997年 | 29篇 |
1996年 | 15篇 |
1995年 | 13篇 |
1994年 | 8篇 |
1993年 | 14篇 |
1992年 | 31篇 |
1991年 | 19篇 |
1990年 | 29篇 |
1989年 | 31篇 |
1988年 | 30篇 |
1987年 | 19篇 |
1986年 | 16篇 |
1985年 | 22篇 |
1984年 | 8篇 |
1983年 | 15篇 |
1982年 | 14篇 |
1981年 | 10篇 |
1980年 | 10篇 |
1979年 | 18篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1976年 | 7篇 |
1975年 | 8篇 |
1974年 | 5篇 |
1969年 | 4篇 |
排序方式: 共有1693条查询结果,搜索用时 15 毫秒
991.
Hundreds of flagellar basal bodies cover the cell surface of the endosymbiotic bacterium Buchnera aphidicola sp. strain APS 下载免费PDF全文
Maezawa K Shigenobu S Taniguchi H Kubo T Aizawa S Morioka M 《Journal of bacteriology》2006,188(18):6539-6543
Buchnera aphidicola is the endosymbiotic bacterium of the pea aphid. Due to its small genome size, Buchnera lacks many essential genes for autogenous life but obtains nutrients from the host. Although the Buchnera cell is nonmotile, it retains clusters of flagellar genes that lack the late genes necessary for motility, including the flagellin gene. In this study, we show that the flagellar genes are actually transcribed and translated and that the Buchnera cell surface is covered with hundreds of hook-basal-body (HBB) complexes. The abundance of HBB complexes suggests a role other than motility. We discuss the possibility that the HBB complex may serve as a protein transporter not only for the flagellar proteins but also for other proteins to maintain the symbiotic system. 相似文献
992.
Yoshida S Tamaoki M Shikano T Nakajima N Ogawa D Ioki M Aono M Kubo A Kamada H Inoue Y Saji H 《Plant & cell physiology》2006,47(2):304-308
Dehydroascorbate reductase (DHAR) is a key component of the ascorbate recycling system. Three functional DHAR genes are encoded in the Arabidopsis genome. Ozone exposure increased the expression of the cytosolic DHAR (cytDHAR) gene alone. We characterized an Arabidopsis mutant with a deficient cytDHAR. The mutant completely lacked cytDHAR activity and was highly ozone sensitive. The amounts of total ascorbate and glutathione were similar in both lines, but the amount of apoplastic ascorbate in the mutant was 61.5% lower. These results indicate that the apoplastic ascorbate, which is generated through the reduction of DHA by cytDHAR, is important for ozone tolerance. 相似文献
993.
Sakata Y Kubo N Morishita M Kitadani E Sugiyama M Hirai M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(2):243-250
A population of F7 recombinant inbred lines (RILs) was made from a cross between susceptible (‘Santou’) and resistant (PI197088-1) lines of
cucumber in order to study powdery mildew resistance loci. Susceptibility to powdery mildew in the F7 RIL individuals showed a continuous distribution from susceptible to resistant, suggesting that powdery mildew resistance
is controlled by quantitative trait loci (QTLs). A QTL analysis identified two and three loci for powdery mildew resistance
under 26 and 20°C conditions, respectively. One QTL was found in the same position under both temperature conditions. Therefore,
it is more likely that one major QTL acts under both temperature conditions and that other QTLs are specific to the two temperature
conditions. The above results suggest that the four QTLs are controlled in a different temperature manner, and that their
combination played an important role in expressing a high level of resistance to powdery mildew in this cucumber population.
Sequence-tagged site (STS) markers associated with each QTL were developed and would be useful for breeding a cucumber line
with a high level of powdery mildew resistance.
Y. Sakata and N. Kubo contributed equally to this work and are considered as first authors. 相似文献
994.
Yumura W Imasawa T Suganuma S Ishigami A Handa S Kubo S Joh K Maruyama N 《Histology and histopathology》2006,21(11):1151-1156
An experimental model with accelerated but not drastic renal senescence seemed useful to recognize the mechanisms of how kidney function deteriorates with age. Senescence marker protein-30 (SMP30), whose expression decreased with age and was sex-independent, is mainly expressed in hepatocytes and proximal tubular cells. Therefore, we established a SMP30 deficient strain of mice with a C57BL/6 background by gene targeting to investigate whether this molecule is involved in renal tubular cell senescence. Male SMP30 knockout (SMP30Y/-) mice and male wild-type (SMPY/+) mice (n=5) aged 12 months were examined histologically. Their tubular epithelia showed the deposition of lipofuscin and the presence of senescence-associated beta-galactosidase (SA-beta-GAL). However, no tubular cells were atrophic. In electron microscopy, SMP30-KO mice showed markedly enlarged lysosomes containing an electron dense substance. These are convincing hallmarks of senescence. We recognized the early manifestation of senescence hallmarks in SMP30-KO mice at 12 months old. Thus, this model represents the first report of a mouse strain that manifests accelerated ordinal senescence in a kidney after gene manipulation. 相似文献
995.
N. N. Skvortsova V. Yu. Korolev T. A. Maravina G. M. Batanov A. E. Petrov A. A. Pshenichnikov K. A. Sarksyan N. K. Kharchev J. Sanchez S. Kubo 《Plasma Physics Reports》2005,31(1):57-74
A new mathematical model is proposed for the probability distributions of the characteristics of the processes observed in turbulent plasmas. The model is based on formal theoretical considerations related to probabilistic limit theorems for a nonhomogeneous random walk and has the form of a finite mixture of Gaussian distributions. The reliability of the model is confirmed by the results of a statistical analysis of the experimental data on density fluctuations in high-temperature plasmas of the L-2M, LHD, and TJ-II stellarators and the local fluctuating flux in the TAU-1 linear device and in the edge plasma of the L-2M stellarator with the use of the estimation-maximization algorithm. It is shown that low-frequency structural turbulence in a magnetized plasma is related to non-Brownian transport, which is determined by the characteristic temporal and spatial scales of the ensembles of stochastic plasma structures. Mechanisms that could be responsible for the random nature of time samples of the local turbulent flux in TAU-1 are indicated. A new physical concept of the intermittence of plasma turbulent pulsations is developed on the basis of the statistical separation of mixtures in terms of the model proposed. The intermittence of plasma pulsations is shown to be associated with the generation of plasma structures (solitons and vortices) and their nonlinear interaction, as well as with their damping and drift. 相似文献
996.
Kubo J Chishaki T Nakamura N Muramatsu T Yamamoto Y Ito M Saitou H Kukidome T 《Journal of strength and conditioning research / National Strength & Conditioning Association》2006,20(3):654-657
The purpose of this study was to investigate differences in fat-free mass and thicknesses of various muscles among judo athletes of different performance levels. The subjects were 69 male judo athletes of 3 different performance levels. Group A was composed of athletes who participated in the Olympic Games or Asian Games (n = 13). Groups B (n = 21) and C (n = 35) were composed of judo athletes at a university who did or did not participate in intercollegiate competitions (including qualifying matches), respectively. Muscle and fat thicknesses were measured by B-mode ultrasound at 9 sites. Fat percentage was calculated from fat thicknesses using a previously reported equation. Fat-free mass was calculated from fat percentage and body weight. Muscles thicknesses were normalized to the height of the individual. Group A had significantly larger fat-free mass than Group C (p < 0.05). The normalized thicknesses of the elbow extensor and flexor muscles were significantly larger in Group A than in Group C. The normalized thickness of the elbow flexor muscle was significantly larger in Group A than in Group B. The results of this study showed that judo athletes with low performance levels such as those in Group C had lower fat-free mass, and the degree of development of the brachialis muscles differed according to performance level. 相似文献
997.
Ogawa D Nakajima N Tamaoki M Aono M Kubo A Kamada H Saji H 《Zeitschrift für Naturforschung. C, Journal of biosciences》2006,61(11-12):856-864
O3 is the major component of photochemical oxidants and gives rise to visible injuries on plant leaves. In O3-exposed plants, O2- is produced before the formation of the injury, but the role that O2- plays in plant response to 03 exposure is still unknown. To clarify its role, we observed the behavior of plants during O3 exposure after pretreatment with tiron, which is an O2- scavenger. When tiron-pretreated tobacco cv. Bel W3 was exposed to O3, leaf damage was attenuated. In O3-exposed tobacco, tiron inhibited increases in the levels of ethylene and salicylic acid, which promote leaf injury. Tiron pretreatment also suppressed increases in the expression of O3-induced genes. These results suggest that O2- is involved in many plant responses induced by O3 exposure. Bel B, a tobacco cultivar that is genetically related to Bel W3, is reported to be more resistant to O3 than Bel W3, but the reason for this difference is unclear. We investigated the differences between the responses of Bel B and tiron-pretreated Bel W3 to O3 exposure, and we discuss the reasons for the resistance to O3 by comparing the phenotype of Bel B with that of tiron-pretreated Bel W3. 相似文献
998.
999.
Mori M Nakajima M Mikami Y Seki S Takigawa M Kubo T Ikegawa S 《Biochemical and biophysical research communications》2006,341(1):121-127
Cartilage intermediate layer protein (CILP) is an extracellular matrix protein abundant in cartilaginous tissues. CILP is implicated in common musculoskeletal disorders, including osteoarthritis and lumbar disc disease. Regulation of the CILP gene is largely unknown, however. We have found that CILP mRNA expression is induced by TGF-β1 and dependent upon signaling via TGF-β receptors. TGF-β1 induction of CILP is mediated by Smad3, which acts directly through cis-elements in the CILP promoter region. Pathways other than Smad3 also are involved in TGF-β1 induction of CILP. These observations, together with the finding that CILP protein binds and inhibits TGF-β1, suggest that CILP and TGF-β1 may form a functional feedback loop that controls chondrocyte metabolism. 相似文献
1000.
A regulatory protein HrpXo of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, is known to control the expression of hrp genes that encode components of a type III secretion system and of some effector protein genes. In this study, we screened novel HrpXo regulons from the genome database of X. oryzae pv. oryzae, searching for ORFs preceded by two predicted sequence motifs, a plant-inducible promoter box-like sequence and a -10 box-like sequence. Using a gus reporter system, nine of 15 ORF candidates were expressed HrpXo dependently. We also showed by base-substituted mutagenesis that both motifs are essential for the expression of the genes. 相似文献