Studies on the aroma of maté (Ilex paraguariensis St. Hil.) using headspace solid-phase microextraction

Volatile and semi-volatile components of maté (Ilex paraguariensis St. Hil.) were analysed by headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography and mass spectrometry. Five SPME fibres coated separately with 100 microm poly(dimethylsiloxane) (PDMS), 65 microm PDMS-divinylbenzene (DVB), 70 microm carbowax (CW)-DVB, 85 microm carboxen (CAR)-PMDS or 50/30 microm DVB-CAR, were tested. Seventy compounds were identified in the sample headspace, including propanal, (E)-2-pentenal, hexanal, (E)-2-hexenal, 6-methyl-5-hepten-2-one, (E,Z)-2,4-heptadienal, (E,E)-2,4-heptadienal, (E,Z)-3,5-octadien-2-one, beta-cyclocitral, 3-ethyl 4-methyl-(1H)-pyrrole-2,5-dione, alpha-ionone, geranylacetone, beta-ionone, dihydroactinidiolide and caffeine. Extraction parameters such as temperature, time and sample mass were studied and optimized. The best conditions for trapping volatile and semi-volatile compounds were obtained using a DVB-CAR fibre at 80 degrees C for 60 min with a sample mass starting from 100 mg in a vial of 4 mL.     

Arsenic tolerance in mesquite (Prosopis sp.): Low molecular weight thiols synthesis and glutathione activity in response to arsenic

The effects of arsenic stress on the production of low molecular weight thiols (LMWT), glutathione S-transferase activity (GST) and sulfur metabolism of mesquite plant (Prosopis sp.) were examined in hydroponic culture at different arsenic [As(III) and (V)] concentrations. The production of LMWT was dependent on As speciation and concentration in the growth medium. The roots of As(III) treated plants produced significantly higher LMWT levels than As(V) treated roots at the same concentration of As applied. In leaves, the thiols content increased with increasing As(III) and (V) concentrations in the medium. Hypersensitivity of the plant to high As concentrations was observed by a significant decrease of LMWT produced in the roots at 50 mg/L treatment in both As(III) and (V) treatments. Sulfur was translocated from roots and accumulated mainly in the shoots. In response to As-induced phytotoxicity, the plants slightly increased the sulfur content in the roots at the highest As treatment. Compared with As(V)-treated plants, As(III)-treated roots and leaves showed significantly higher GST activity. The roots of both As(III) and (V) treated plants showed an initial increase in GST at low As concentration (5 mg/L), followed by significant inhibition up to 50 mg/L. The leaves had the highest GST activity, an indication of the ability of the plant to detoxify As in the leaves than in the roots. The correlation between LMWT content, S content and GST activity may be an indication these parameters may be used as biomarkers of As stress in mesquite.     

Theory of antigen-antibody induced particulate aggregation—II theoretical analysis and comparison with experimental results

The roles of the concentrations of the three interacting constituents in the aggregation process (antibodies, antigens and particulates) are analyzed in detail. It is shown that the basic equation derived in Part I is consistent over a broad range of conditions with experimental findings previously reported.     

Landscape topography and the distribution of Lahontan cutthroat trout (Oncorhynchus clarki henshawi) in a high desert stream

Lahontan cutthroat trout, Oncorhynchus clarki henshawi, are currently limited in their distribution to a patchwork of small isolated populations, the result of habitat degradation and natural variation in landscape and in-stream conditions. The objectives of this study were to determine if landscape topography influences trout distribution, and if water temperatures control this response. The work was carried out in a sub-basin of the Quinn River system, McDermitt Creek, which drains the sagebrush desert of southeastern Oregon and northern Nevada. Headwater tributaries of this creek consist of alternating canyon-confined and valley bounded reaches. Trout within these systems are challenged by low discharge and high temperatures during the summer, and anchor ice during the winter. Contiguous whole stream surveys were used to look at trout distribution during the summer of 2003 and spring and fall of 2004. Our results suggested that topography can affect trout distribution. Trout numbers were highest in areas with greater numbers of nick-points (the transition zones between less confined and more confined valley segments) and greater valley confinement. Additionally, in the downstream portion of our headwater reaches, more trout were found in nick-points than expected based on the availability of this habitat type. Our data suggest that hyporheic inputs may be high in such areas, thus providing trout with shelter from warm water in the summer, anchor ice in the winter, and shallow stream depths during all seasons. Spatial occurrence of these areas of refugia can be taken into consideration when planning land use activities and restoration efforts. Further research is required to confirm that topography can affect the distribution of Lahontan cutthroat trout in other systems, and to better understand the mechanisms behind these patterns.     

Catalysis of thiol/disulfide exchange. Glutaredoxin 1 and protein-disulfide isomerase use different mechanisms to enhance oxidase and reductase activities

Glutaredoxin (Grx) and protein-disulfide isomerase (PDI) are members of the thioredoxin superfamily of thiol/disulfide exchange catalysts. Thermodynamically, rat PDI is a 600-fold better oxidizing agent than Grx1 from Escherichia coli. Despite that, Grx1 is a surprisingly good protein oxidase. It catalyzes protein disulfide formation in a redox buffer with an initial velocity that is 30-fold faster than PDI. Catalysis of protein and peptide oxidation by the individual catalytic domains of PDI and by a Grx1-PDI chimera show that differences in active site chemistry are fundamental to their oxidase activity. Mutations in the active site cysteines reveal that Grx1 needs only one cysteine to catalyze rapid substrate oxidation, whereas PDI requires both cysteines. Grx1 is a good oxidase because of the high reactivity of a Grx1-glutathione mixed disulfide, and PDI is a good oxidase because of the high reactivity of the disulfide between the two active site cysteines. As a protein disulfide reductase, Grx1 is also superior to PDI. It catalyzes the reduction of nonnative disulfides in scrambled ribonuclease and protein-glutathione mixed disulfides 30-180 times faster than PDI. A multidomain structure is necessary for PDI to catalyze effective protein reduction; however, placing Grx1 into the PDI multidomain structure does not enhance its already high reductase activity. Grx1 and PDI have both found mechanisms to enhance active site reactivity toward proteins, particularly in the kinetically difficult direction: Grx1 by providing a reactive glutathione mixed disulfide to supplement its oxidase activity and PDI by utilizing its multidomain structure to supplement its reductase activity.     

Muscle-specific microtubule-associated protein 4 is expressed early in myogenesis and is not sufficient to induce microtubule reorganization

The expression of a muscle-specific variant of microtubule-associated protein 4 (mMAP4) has been analyzed during myogenesis of C(2)C(12) cells using an isoform-specific antibody. MMAP4 localizes to microtubules (MTs) and is expressed prior to a very early morphogenetic event, the formation of mononucleate spindle-shaped cells. MMAP4 protein appears at about the same time as titin and coincident with Golgi reorganization, but antedates myosin expression. Misexpression of EGFP-mMAP4 in non-muscle and proliferating C(2)C(12) cells does not induce dramatic changes in MT organization or stability, nor in Golgi organization. Expression of full-length mMAP4 or of a truncated form lacking the MT-binding domain does not disrupt myotube formation or myofibrillogenesis. While previous antisense studies indicated that mMAP4 is necessary for normal myotube formation [Mangan and Olmsted, 1996: Development 122:771-781], these data indicate mMAP4 is not sufficient to induce the reorganization of MTs or the Golgi into patterns typical of muscle cells. Thus, with respect to MT organizing properties, this tissue-specific variant differs from related neuronal MAPs, MAP2, and tau, which induce neural-like changes in MT organization.     

Oak galls preserved by the eruption of mount vesuvius in A.D. 79, and their probable use

Eight oak galls caused by cynipid wasps and preserved by the eruption of Mt. Vesuvius at Herculaneum, Italy, provide tangible clues to the significance of galls in early human culture. The external and internal appearances of the galls are described. Remnants of insects were found in two of the specimens. It is suggested that all but one of the galls were caused by the cynipidAdleria kollari. Probable uses of the galls are discussed; it is concluded that the galls were likely sold for use in medicines.