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排序方式: 共有92条查询结果,搜索用时 23 毫秒
51.
Jordi Corominas Jorge AP Marchesi Anna Puig-Oliveras Manuel Revilla Jordi Estellé Estefania Alves Josep M Folch Maria Ballester 《遗传、选种与进化》2015,47(1)
Background
In previous studies on an Iberian x Landrace cross, we have provided evidence that supported the porcine ELOVL6 gene as the major causative gene of the QTL on pig chromosome 8 for palmitic and palmitoleic acid contents in muscle and backfat. The single nucleotide polymorphism (SNP) ELOVL6:c.-533C > T located in the promoter region of ELOVL6 was found to be highly associated with ELOVL6 expression and, accordingly, with the percentages of palmitic and palmitoleic acids in longissimus dorsi and adipose tissue. The main goal of the current work was to further study the role of ELOVL6 on these traits by analyzing the regulation of the expression of ELOVL6 and the implication of ELOVL6 polymorphisms on meat quality traits in pigs.Results
High-throughput sequencing of BAC clones that contain the porcine ELOVL6 gene coupled to RNAseq data re-analysis showed that two isoforms of this gene are expressed in liver and adipose tissue and that they differ in number of exons and 3’UTR length. Although several SNPs in the 3’UTR of ELOVL6 were associated with palmitic and palmitoleic acid contents, this association was lower than that previously observed with SNP ELOVL6:c.-533C > T. This SNP is in full linkage disequilibrium with SNP ELOVL6:c.-394G > A that was identified in the binding site for estrogen receptor alpha (ERα). Interestingly, the ELOVL6:c.-394G allele is associated with an increase in methylation levels of the ELOVL6 promoter and with a decrease of ELOVL6 expression. Therefore, ERα is clearly a good candidate to explain the regulation of ELOVL6 expression through dynamic epigenetic changes in the binding site of known regulators of ELOVL6 gene, such as SREBF1 and SP1.Conclusions
Our results strongly suggest the ELOVL6:c.-394G > A polymorphism as the causal mutation for the QTL on pig chromosome 8 that affects fatty acid composition in pigs.Electronic supplementary material
The online version of this article (doi:10.1186/s12711-015-0111-y) contains supplementary material, which is available to authorized users. 相似文献52.
53.
GV Pavlova AA Vergun EY Rybalkina PR Butovskaya AP Ryskov 《Cell cycle (Georgetown, Tex.)》2015,14(2):200-205
Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells demonstrated increased DNA instability. RAPD fragments from different cell lines after different number of passages were cloned and sequenced. The chromosomal localization of these fragments was identified and single-nucleotide variations in RAPD fragments isolated from cell lines after 8–12 passages were revealed. Some of them had permanent localization, while most variations demonstrated random distribution and can be considered as de novo mutations. 相似文献
54.
A Dawson S A Hinsley P N Ferns R H Bonser L Eccleston 《Proceedings. Biological sciences / The Royal Society》2000,267(1457):2093-2098
Life-history theory proposes that costs must be associated with reproduction. Many direct costs are incurred during breeding. There is also evidence for indirect costs, incurred after breeding, which decrease survival and future reproductive success. One possible indirect cost identified in birds is that breeding activity in some way compromises plumage quality in the subsequent moult. Here we propose a mechanism by which this could occur. Breeding activity delays the start of moult. Birds that start to moult later also moult more rapidly--an effect of decreasing daylength. Could this result in poorer quality plumage? We kept two groups of male European starlings, Sturnus vulgaris, one on constant long days and the other on decreasing daylengths from the start of moult. Decreasing daylengths reduced the duration of moult from 103 +/- 4 days to 73 +/- 3 days (p < 0.0001). Newly grown primary feathers of birds that moulted fast were slightly shorter, weighed less (p < 0.05) and were more asymmetrical. They had a thinner rachis (p < 0.005), were less hard (p < 0.01) and less rigid (p < 0.05). They were also less resistant to wear so that differences in mass and asymmetry increased with time. There was no difference in Young''s modulus. Poorer quality plumage will lead to decreased survival due to decreased flight performance and increased thermoregulatory costs. Thus, reproduction incurs costs through a mechanism that operates after the end of breeding. 相似文献
55.
David Lee Tjaart AP de Beer Roman A Laskowski Janet M Thornton Christine A Orengo 《BMC structural biology》2011,11(1):2
Background
The Midwest Center for Structural Genomics (MCSG) is one of the large-scale centres of the Protein Structure Initiative (PSI). During the first two phases of the PSI the MCSG has solved over a thousand protein structures. A criticism of structural genomics is that target selection strategies mean that some structures are solved without having a known function and thus are of little biomedical significance. Structures of unknown function have stimulated the development of methods for function prediction from structure. 相似文献56.
S Çolakoğlu A Aktaş S Raimondo AP Türkmen BZ Altunkaynak E Odacı 《Biotechnic & histochemistry》2014,89(2):136-144
We investigated the effects of diclofenac sodium (DS) on development of the optic nerve in utero. Pregnant female rats were separated into three groups: control, saline treated and DS treated. Offspring of these animals were divided into 4-week-old and 20-week-old groups. At the end of the 4th and 20th weeks of postnatal life, the animals were sacrificed, and right optic nerves were excised and sectioned for ultrastructural and stereological analyses. We demonstrated that both DS and saline produced structural and morphometric changes in the total axon number and density of axons, but decreased the myelin sheath thickness in male optic nerves. All ultrastructural and morphometric features were well developed in 20-week-old rats. We showed that development of the optic nerve continues during the early postnatal period and that some compensation for exposure to deleterious agents in utero may occur during early postnatal life. 相似文献
57.
Network analysis of temporal functionalities of the gut induced by perturbations in new-born piglets
Nirupama Benis Dirkjan Schokker Maria Suarez-Diez Vitor AP Martins dos Santos Hauke Smidt Mari A Smits 《BMC genomics》2015,16(1)
Background
Evidence is accumulating that perturbation of early life microbial colonization of the gut induces long-lasting adverse health effects in individuals. Understanding the mechanisms behind these effects will facilitate modulation of intestinal health. The objective of this study was to identify biological processes involved in these long lasting effects and the (molecular) factors that regulate them. We used an antibiotic and the same antibiotic in combination with stress on piglets as an early life perturbation. Then we used host gene expression data from the gut (jejunum) tissue and community-scale analysis of gut microbiota from the same location of the gut, at three different time-points to gauge the reaction to the perturbation. We analysed the data by a new combination of existing tools. First, we analysed the data in two dimensions, treatment and time, with quadratic regression analysis. Then we applied network-based data integration approaches to find correlations between host gene expression and the resident microbial species.Results
The use of a new combination of data analysis tools allowed us to identify significant long-lasting differences in jejunal gene expression patterns resulting from the early life perturbations. In addition, we were able to identify potential key gene regulators (hubs) for these long-lasting effects. Furthermore, data integration also showed that there are a handful of bacterial groups that were associated with temporal changes in gene expression.Conclusion
The applied systems-biology approach allowed us to take the first steps in unravelling biological processes involved in long lasting effects in the gut due to early life perturbations. The observed data are consistent with the hypothesis that these long lasting effects are due to differences in the programming of the gut immune system as induced by the temporary early life changes in the composition and/or diversity of microbiota in the gut.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1733-8) contains supplementary material, which is available to authorized users. 相似文献58.
Shelley A. Hinsley Paul E. Bellamy Peter Rothery Paula Redman Lindsay Furness John R. Speakman 《Bird Study》2013,60(2):151-159
Capsule Female feeding rate behaviour was highly variable between individuals but no effect of the doubly labelled water (DLW) procedure on female visit rate was detected, whereas visit rates by males, which were neither trapped or manipulated, increased following trapping and manipulation of their partners. Aims To test the assumption that the subject's behaviour is normal during the measurement period when using DLW to measure energy expenditure. Methods Visits to the nestbox by parents feeding young were counted separately for females and males on the day before and the day after the female only was trapped to measure her energy expenditure using DLW. Visit rates were also counted for control pairs. Results Female visit rates did not differ before and after manipulation, or between experimental and control pairs, but bird behaviour was highly variable between individuals. In contrast, the visit rates of both experimental and control males, which were not trapped, increased on the second day. Conclusion The results for female behaviour supported the assumption of normality, but a small subset of particular individuals may be prone to adverse reactions. The response of the males may have been a reaction to disturbance at the nest. 相似文献
59.
60.
Flávia C Rodrigues-Lisoni Paulo PeitlJr Alessandra Vidotto Giovana M Polachini José V Maniglia Juliana Carmona-Raphe Bianca R Cunha Tiago Henrique Caique F Souza Rodrigo AP Teixeira Erica E Fukuyama Pedro MichaluartJr Marcos B de Carvalho Sonia M Oliani Head Neck Genome Project GENCAPO Eloiza H Tajara 《BMC medical genomics》2010,3(1):14