全文获取类型
收费全文 | 295篇 |
免费 | 17篇 |
专业分类
312篇 |
出版年
2022年 | 4篇 |
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 12篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 9篇 |
2014年 | 21篇 |
2013年 | 13篇 |
2012年 | 20篇 |
2011年 | 9篇 |
2010年 | 16篇 |
2009年 | 7篇 |
2008年 | 14篇 |
2007年 | 15篇 |
2006年 | 18篇 |
2005年 | 5篇 |
2004年 | 7篇 |
2003年 | 13篇 |
2002年 | 5篇 |
1998年 | 6篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1992年 | 6篇 |
1991年 | 2篇 |
1990年 | 7篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1978年 | 1篇 |
1977年 | 4篇 |
1974年 | 4篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 3篇 |
1968年 | 4篇 |
1966年 | 1篇 |
1943年 | 2篇 |
1940年 | 2篇 |
1939年 | 1篇 |
1929年 | 1篇 |
1904年 | 1篇 |
1902年 | 1篇 |
排序方式: 共有312条查询结果,搜索用时 15 毫秒
71.
Blažena Pekárková Jan Šmarda František Hindák 《Plant Systematics and Evolution》1989,164(1-4):263-272
Four unialgal strains of the freshwater coccoid red algaPorphyridium aerugineum
Geitler were cultivated under laboratory conditions. Cell morphology was studied with the light microscope. The cell surface was examined by means of electron microscopy in order to contribute to the knowledge of polysaccharide sheaths and cytoplasmic membranes. Optimum growth conditions were determined. The range of cell sizes and the average dry masses of single cells were compared in all four strains cultivated at exactly defined temperatures and irradiances. Photosynthetic pigment maxima were measured in intact cells. The red-coloured phycobiliprotein phycoerythrin was not found in any of the examined strains.Dedicated to Prof. DrLothar Geitler on the occasion of the 90th anniversary of his birthday. 相似文献
72.
Kim Anh Nguyen Hind Hamzeh-Cognasse Sabine Palle Isabelle Anselme-Bertrand Charles-Antoine Arthaud Patricia Chavarin Bruno Pozzetto Olivier Garraud Fabrice Cognasse 《PloS one》2014,9(9)
Background
Platelets participate in tissue repair and innate immune responses. Sialic acid-binding immunoglobulin-like lectins (Siglecs) are well-characterized I-type lectins, which control apoptosis.Methodology/Principal Findings
We characterized the expression of Siglec-7 in human platelets isolated from healthy volunteers using flow cytometry and confocal microscopy. Siglec-7 is primarily expressed on α granular membranes and colocalized with CD62P. Siglec-7 expression was increased upon platelet activation and correlated closely with CD62P expression. Cross-linking Siglec-7 with its ligand, ganglioside, resulted in platelet apoptosis without any significant effects on activation, aggregation, cell morphology by electron microscopy analysis or secretion. We show that ganglioside triggered four key pathways leading to apoptosis in human platelets: (i) mitochondrial inner transmembrane potential (ΔΨm) depolarization; (ii) elevated expression of pro-apoptotic Bax and Bak proteins with reduced expression of anti-apoptotic Bcl-2 protein; (iii) phosphatidylserine exposure and (iv), microparticle formation. Inhibition of NAPDH oxidase, PI3K, or PKC rescued platelets from apoptosis induced by Siglec-7 recruitment, suggesting that the platelet receptors P2Y1 and GPIIbIIIa are essential for ganglioside-induced platelet apoptosis.Conclusions/Significance
The present work characterizes the role of Siglec-7 and platelet receptors in regulating apoptosis and death. Because some platelet pathology involves apoptosis (idiopathic thrombocytopenic purpura and possibly storage lesions), Siglec-7 might be a molecular target for therapeutic intervention/prevention. 相似文献73.
G Hind 《Biochimica et biophysica acta》1968,153(1):235-240
74.
75.
76.
The organization of filamentous actin (F-actin) in living cells of the oomycete Phytophthora cinnamomi was determined during zoosporogenesis and zoospore encystment by microinjecting sporangia with fluorescently labeled phalloidin and observing resultant fluorescence by confocal microscopy. In multinucleate sporangia prior to the induction of cleavage, phalloidin labeling took the form of plaques which occurred mainly in the periphery of the sporangia. After induction of cleavage, phalloidin labeling showed that the plaques disappeared and that F-actin began to accumulate along the developing cleavage planes and around nuclei and water expulsion vacuoles. F-actin labeling was also observed near the plasma membrane in zoospores and young cysts but reverted to the plaque form in older cysts. Localization of F-actin close to the developing cleavage planes is consistent with the idea that actin microfilaments function in the positioning and expansion of the cleavage membranes. Observations of plaques of actin in living sporangia provide evidence that plaques are not aldehyde-induced fixation artifacts. Copyright 1998 Academic Press. 相似文献
77.
Serglycin is the major proteoglycan in most hematopoietic cells, including
monocytes and macrophages. The monoblastic cell line U937-1 was used to
study the expression of serglycin during proliferation and differentiation.
In unstimulated proliferating U937-1 cells serglycin mRNA is
nonconstitutively expressed. The level of serglycin mRNA was found to
correlate with the synthesis of chondroitin sulfate proteoglycan (CSPG).
The U937-1 cells were induced to differentiate into different types of
macrophage-like cells by exposing the cells to PMA, RA, or VitD3. These
inducers of differentiation affected the expression of serglycin mRNA in
three different ways. The initial upregulation seen in the normally
proliferating cells was not observed in PMA treated cells. In contrast, RA
increased the initial upregulation, giving a reproducible six times
increase in serglycin mRNA level from 4 to 24 h of incubation, compared to
a four times increase in the control cells. VitD3 had no effect on the
expression of serglycin mRNA. The incorporation of (35S)sulfate into CSPG
decreased approximately 50% in all three differentiated cell types.
Further, the (35S)CSPGs expressed were of larger size in PMA treated cells
than controls, but smaller after RA treatment. This was due to the
expression of CSPGs, with CS-chains of 25 and 5 kDa in PMA and RA treated
cells, respectively, compared to 11 kDa in the controls. VitD3 had no
significant effect on the size of CSPG produced. PMA treated cells secreted
75% of the (35S)PGs expressed, but the major portion was retained in cells
treated with VitD3 or RA. The differences seen in serglycin mRNA levels,
the macromolecular properties of serglycin and in the PG secretion
patterns, suggest that serglycin may have different functions in different
types of macrophages.
相似文献
78.
The kinetic behaviours of cytochrome -563 and cytochrome are shown to be consistent with their participation in coupled cyclic electron flow in intact chloroplasts. Electron transfer between cytochromes -563 and cytochrome is antimycin sensitive. Fluorescence induction studies indicate that plastoquinone may function in a coupled step between the cytochromes. 相似文献
79.
80.