全文获取类型
收费全文 | 659篇 |
免费 | 89篇 |
出版年
2023年 | 4篇 |
2022年 | 3篇 |
2021年 | 16篇 |
2020年 | 8篇 |
2019年 | 7篇 |
2018年 | 11篇 |
2017年 | 12篇 |
2016年 | 15篇 |
2015年 | 23篇 |
2014年 | 23篇 |
2013年 | 27篇 |
2012年 | 55篇 |
2011年 | 62篇 |
2010年 | 17篇 |
2009年 | 19篇 |
2008年 | 39篇 |
2007年 | 35篇 |
2006年 | 46篇 |
2005年 | 26篇 |
2004年 | 27篇 |
2003年 | 29篇 |
2002年 | 27篇 |
2001年 | 16篇 |
2000年 | 17篇 |
1999年 | 19篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 9篇 |
1995年 | 8篇 |
1994年 | 9篇 |
1993年 | 7篇 |
1992年 | 15篇 |
1991年 | 10篇 |
1990年 | 9篇 |
1989年 | 9篇 |
1988年 | 5篇 |
1987年 | 4篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1973年 | 3篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1961年 | 2篇 |
排序方式: 共有748条查询结果,搜索用时 15 毫秒
741.
742.
Effect of Planting Season, Bunchgrass Species, and Neighbor Control on the Success of Transplants for Grassland Restoration 总被引:1,自引:0,他引:1
Constraints to grassland and open forest restoration (e.g., poor seed sources, yearly variation in establishment, and the persistence of weeds) necessitate the development of innovative methods to restore bunchgrass communities. We assessed the use of two native bunchgrass transplants, Bluebunch wheatgrass (Pseudoroegneria spicata) and Spreading needlegrass (Achnatherum richardsonii), for restoration within thinned montane forest communities of southeastern British Columbia, Canada. Fall and spring plantings were examined, either with or without glyphosate treatments to Pinegrass (Calamagrostis rubescens) neighbors. Calamagrostis rubescens is abundant in grassland affected by tree encroachment and may limit transplant establishment. Bunchgrass survival was positively associated (p < 0.05) with transplant size. Although P. spicata survival was greater (p < 0.01) with fall (81%) than with spring (44%) planting, survival of A. richardsonii was greater (p < 0.01) when planted in the spring (68 vs. 23%). Reduction of C. rubescens led to a relatively small but significant increase (p < 0.05) in bunchgrass survival by 7%. The summer after planting, changes in transplant tiller number varied by bunchgrass species, planting season, and treatment of neighboring C. rubescens. Removal of neighboring C. rubescens generally increased the number of tillers (or reduced tiller loss) but only within fall‐planted A. richardsonii and spring‐planted P. spicata. Both A. richardsonii and P. spicata transplants have potential for understory restoration within thinned montane forests, particularly using larger individuals, although to maximize survival, these species should be planted in the spring and fall, respectively. Reduction of C. rubescens may also enhance transplant survival and in some cases growth. 相似文献
743.
Recently, training programs in research ethics have been established to enhance individual and institutional capacity in research ethics in the developing world. However, commentators have expressed concern that the efforts of these training programs have placed ‘too great an emphasis on guidelines and research ethics review’, which will have limited effect on ensuring ethical conduct in research. What is needed instead is a culture of ethical conduct supported by national and institutional commitment to ethical practices that are reinforced by upstream enabling conditions (strong civil society, public accountability, and trust in basic transactional processes), which are in turn influenced by developmental conditions (basic freedoms of political freedoms, economic facilities, social opportunities, transparency guarantees, and protective security). Examining this more inclusive understanding of the determinants of ethical conduct enhances at once both an appreciation of the limitations of current efforts of training programs in research ethics and an understanding of what additional training elements are needed to enable trainees to facilitate national and institutional policy changes that enhance research practices. We apply this developmental model to a training program focused in Egypt to describe examples of such additional training activities. 相似文献
744.
Glycosylation is one of the most fundamental posttranslational modifications in cellular biology and has been shown to be epigenetically regulated. Understanding this process is important as epigenetic therapies such as those using DNA methyltransferase inhibitors are undergoing clinical trials for the treatment of ovarian and breast cancer. Previous work has demonstrated that altered glycosylation patterns are associated with aggressive disease in women presenting with breast and ovarian cancer. Moreover, the tumor microenvironment of hypoxia results in globally altered DNA methylation and is associated with aggressive cancer phenotypes and chemo-resistance, a feature integral to many cancers. There is sparse knowledge on the impact of these therapies on glycosylation. Moreover, little is known about the efficacy of DNA methyltransferase inhibitors in hypoxic tumors. In this review, we interrogate the impact that hypoxia and epigenetic regulation has on cancer cell glycosylation in relation to resultant tumor cell aggressiveness and chemo-resistance. 相似文献
745.
746.
Rifampin, an antibiotic which is known to bind to and inhibit RNA polymerase, was used to probe the molecular regulation of development in Myxococcus xanthus. Rifampin-resistant mutants were screened for defects in fruiting-body formation. About 20% of the isolates in the initial screenings showed major defects in developmental aggregation or sporulation. Eleven independent mutants with wild-type growth rates and stable phenotypes were analyzed by transduction. In these strains, the rifampin-resistant and nonfruiting phenotypes showed cotransduction frequencies equal to or greater than 99.0 to 99.9%. The RNA polymerase activities were resistant to rifampin in vitro, indicating that the RNA polymerase is altered in these strains. Although their fruiting phenotypes are heterogeneous, these strains can be divided into two classes based on the level of aggregation. The results suggest that RNA polymerase plays a significant role in the regulation of development in M. xanthus since mutations which cause no apparent changes in vegetative growth result in striking defects in fruiting-body formation. 相似文献
747.
748.
B. J. Trask Heather Mefford Ger van den Engh Hillary F. Massa Ramesh C. Juyal Lorraine Potocki Brenda Finucane Dianne N. Abuelo David R. Witt Ellen Magenis Antonio Baldini Frank Greenberg James R. Lupski P. I. Patel 《Human genetics》1996,98(6):710-718
We have used bivariate flow karyotyping to quantify the deletions involving chromosome 17 in sixteen patients with Smith-Magenis
syndrome (SMS). The fluorescence intensities of mitotic chromosomes stained with Hoechst 33258 and chromomycin were quantified
in a dual-beam flow cytometer. For each patient, the position of the peak representing the deleted chromosome 17 was compared
to those of the normal homologs of an unaffected parent. The patients could be classified into four groups based on the size
of their deletions. The deletions ranged from ∼9–10 Mb (∼10–11% of the chromosome) to below the detection limit of the technique
(2 Mb). Different deletion sizes were detected among patients whose high-resolution banding results were similar. Some deletions
detected by banding were not detected by flow analyses. Deletion estimates are largely consistent with the results of molecular
analyses. Patients with larger deletions that extend into band 17p12 have abnormal electrophysiologic studies of peripheral
nerves. Deletion size does not appear to correlate with the degree of mental retardation, presence of behavioral abnormalities,
craniofacial anomalies or common skeletal findings in SMS. By identifying patients with varying deletion sizes, these data
will aid the construction of a long-range deletion-based map of 17p11.2 and identification of the genes involved in this syndrome.
Received: 19 March 1996 / Revised: 21 June 1996 相似文献