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排序方式: 共有593条查询结果,搜索用时 15 毫秒
91.
S. Massa R. Armuzzi M. Tosques F. Canganella L. D. Trovatelli 《Journal of applied microbiology》1999,86(1):169-173
The susceptibility of five Aeromonas hydrophila strains and one Escherichia coli strain to chlorine was studied under carefully controlled laboratory conditions. Of the Aer. hydrophila strains, two were from untreated water, two from tap water (immediately downstream of a water treatment plant) and one from the DSM collection. The study included disinfectant concentration (0·1, 0·2 and 0·5 mg l−1 ), pH (6, 7 and 8) and temperature (4, 21 and 32 °C) as controlled variables. The results indicated that the untreated water strains, the DSM strain and the E. coli strain were inactivated within 1 min of chlorine treatment. The strains from chlorinated water (TW11 and TW27) showed a different susceptibility to chlorine disinfection, the rate of inactivation being greater at pH 6 than at pH 8 for both strains. Under the standard conditions of temperature 21 °C, pH 7 and chlorine concentration 0·2 mg l−1 , an increase or decrease of approximately 1 log unit in the number of bacteria did not affect the kill rate of the strains TW11 and TW27. 相似文献
92.
This is a preliminary study of the duration of marriages and remarriages by widowed people in Chiomonte (Turin) from 1670
to 1729, a period that saw long periods of war alternating with brief intervals of peace.
We analysed 748 marriage acts and evaluated the age at first marriage, the duration of marriage, the frequency of remarriage
by widowed people and the number of widowed people who remarried. The data were analysed in five-year intervals. The results
show that the population of Chiomonte was essentially permanent, since the end of the marriage could be established in 77,6%
of cases. The mean duration of marriages increased from the end of the XVII century and around 51% of all marriages lasted
more than 20 years.
Historical events had different effects on the matrimonial behaviour. After the mortality crisis in 1690–91, marriages and
remarriages increased in 1691–94, a typical response of populations decimated by an epidemic. However, the matrimonial behaviour
in the decade 1704–14 was different, in that there was a strong increase in exogamous marriages (56.52% of all marriages).
Remarriages, especially of widowed persons, also increased in that period.
Although these data are preliminary and from a brief time period, they reveal particular aspects of the marriage and remarriage
behaviour, thus helping to clarify the biodemographic evolution of the Chiomonte population. 相似文献
93.
Heterologous expression of Mytilus californianus foot protein three (Mcfp-3) in Kluyveromyces lactis
Platko JD Deeg M Thompson V Al-Hinai Z Glick H Pontius K Colussi P Taron C Kaplan DL 《Protein expression and purification》2008,57(1):57-62
Mytilus californianus foot protein three (Mcfp-3) was successfully expressed in the yeast, Kluyveromyces lactis. The first nine amino acids (YPYDVPDYA) from the human-influenza-virus hemagglutinin (HA) protein were fused to the amino terminus of Mcfp-3 (HA-Mcfp-3) to facilitate identification and purification. HA-Mcfp-3 was purified to a concentration of 1mg/L using HA affinity chromatography. The recovered polypeptide was resolved by SDS-PAGE and migrated primarily at 36 kDa, an increase of approximately 29 kDa over the calculated molecular weight of a HA-Mcfp-3 monomer. Significantly, release of Mcfp-3 by enterokinase treatment coincided with the formation of high molecular weight complexes. It is noteworthy that the complexes mimicked the previously reported insolubility of Mcfps found in vivo to denaturing and reducing conditions. These data demonstrate the successful expression of Mcfp-3 in K. lactis and show an intrinsic ability of Mcfp-3 to self-assemble into stable, higher molecular weight forms. 相似文献
94.
Mai A Perrone A Nebbioso A Rotili D Valente S Tardugno M Massa S De Bellis F Altucci L 《Bioorganic & medicinal chemistry letters》2008,18(8):2530-2535
A novel series of non-hydroxamate HDAC inhibitors (HDACi) showing a uracil group at the left and a 2-aminoanilide/2-aminoanilide-like portion at the right head have been reported. In particular, the new compounds incorporating a 2-aminoanilide moiety behaved as class I-selective HDACi. Compound 8, the most potent and class I-selective, showed weak apoptosis (higher than MS-275) joined to cytodifferentiating activity on U937 cells. Surprisingly, the highest differentiation was observed with 13, through an effect that seems to be unrelated to HDAC inhibition. 相似文献
95.
A. Incarbona S. Bonomo E. Di Stefano S. Zgozi N. Essarbout M. Talha G. Tranchida A. Bonanno B. Patti F. Placenti G. Buscaino A. Cuttitta G. Basilone T. Bahri F. Massa P. Censi S. Mazzola 《Marine Micropaleontology》2008,67(3-4):297-309
Quantitative analysis of 67 calcareous nannofossil assemblages from surface sediments recovered in a wide area across the Sicily Channel has been carried out in order to improve the interpretation of palaeontological data based on this planktonic group in a key area for Mediterranean palaeoceanographic studies. The investigation focused on three case studies that demonstrate the high potentiality of such a combined approach, taking into account the recent distribution of taxa or groups of taxa on the sea floor and the palaeontological record. The distribution of reworked specimens over the northern Sicily Channel sea floor validates the role of southern Sicily as a source region for reworked nannofossils and the role of rivers as their carrier. Eustatic sea-level fluctuations can be considered to be the main factor that influenced the abundance variations in sedimentary sequences of this area. The distribution of Florisphaera profunda can be explained in terms of topography (positive correlation) and mesoscale oceanographic circulation. In particular, its significant anti-correlation to the amount of chlorophyll-A deduced by satellite imagery validates the use of this species as a proxy for palaeoproductivity reconstructions. Finally, high abundance values of G. oceanica are confined to the westernmost part of the Sicily Channel, coinciding with a water mass salinity minimum. In particular, abundances of up to about 10% were observed in the westernmost part of the African Margin, suggesting the importance of the Atlantic Tunisian Current, whose activity is more pronounced in winter. The comparison of data of this species between 135 and 110 kyr BP, inside and outside the Channel, led us to deduce that the physical transport in almost unmodified waters of Atlantic origin might be the most important factor for its significant occurrence. 相似文献
96.
Denise Friselli Carlo-Andrea Massa Massimo Martinelli Luca Pardi Irene Ricci 《Inorganica chimica acta》2008,361(14-15):4164-4166
The high field EPR spectra of non-diluted magnetic material are affected by propagation effects when the wavelength of the exciting radiation is of the same order of magnitude of the optical path within the sample. Beyond the optical path, the shape of the spectra is determined by the dielectric constant and by the magnetization of the material and through these quantities it depends on the temperature. A detailed knowledge of the physical properties of the material is therefore mandatory for a complete study of the phenomenon. In order to demonstrate the propagation effect, the 285 GHz EPR spectra of tetramethyl-ammonium manganese chloride (TMMC) were recorded as a function of temperature and a simulation of the spectra was performed on the basis of a simplified model of the propagation of far infrared radiation around the resonance field. The universality of the effect was illustrated by measuring other magnetic materials such as ferrite. 相似文献
97.
Hoffman HE Jirásková J Ingr M Zvelebil M Konvalinka J 《Protein expression and purification》2009,63(1):62-67
D-serine plays a key role in glutamatergic neurotransmission in mammalian brain as a co-agonist of N-methyl-D-aspartate receptors. The enzyme responsible for D-serine biosynthesis, serine racemase (SR), is therefore a promising target for treatment of neuropathologies related to glutamate receptor excitotoxicity, such as stroke or Alzheimer's disease. Much of the experimental work to date has been performed on mouse serine racemase, which shares a high level of sequence identity with its human ortholog. In this work, we report the synthesis of a human SR gene variant optimized for heterologous expression in Escherichia coli and describe the expression and purification of active recombinant human SR. This strategy may be of general interest to researchers wishing to express mammalian proteins in a bacterial system. Furthermore, we conduct a thorough analysis of the kinetics and inhibitor-sensitivity of the recombinant enzyme, and we provide the first direct comparison of human and mouse SR based on our kinetic data. The orthologs behave similarly overall and exhibit identical inhibition profiles, validating the use of mouse models in SR research. 相似文献
98.
Matteo Anselmino Maria Cristina Marocco Marcella Jorfida Riccardo Massa 《Indian pacing and electrophysiology journal》2009,9(3):177-179
Transvenous endocardial pacing through classical implantation of a pace/sensing lead in the right ventricle is strictly contraindicated in patients with a mechanical tricuspid valve. Usually permanent pacing is achieved by an epimyocardial surgical approach. We hereby describe the implantation of a single site left ventricle pacing lead in the anterior interventricular vein in a 60 year-old woman with symptomatic bradycardia, permanent atrial fibrillation, and mechanical tricuspid valve. The described use of left ventricle pacing through a coronary vein lead, in a patient with favorable venous anatomy, provided (through a minimal invasive approach) effective with a low and stable threshold. 相似文献
99.
Massa K Magnussen P Sheshe A Ntakamulenga R Ndawi B Olsen A 《Journal of biosocial science》2009,41(1):89-105
The success of the Community-Directed Treatment (ComDT) approach in the control of onchocerciasis and filariasis has caught the attention of other disease control programmes. In this study the ComDT approach was implemented and compared with the school-based approach for control of schistosomiasis and soil-transmitted helminthiasis among school-age children in Lushoto District, Tanzania. This was a qualitative study, consisting of in-depth interviews with village leaders, community drug distributors (CDDs) and schoolteachers, as well as focus group discussions with separate groups of mothers and fathers to assess the perceptions and experiences of the villagers on the implementation of the two approaches. It was found that the villagers accepted the ComDT approach and took the responsibility of selecting the CDDs, organizing and implementing their own method of distributing drugs to the school-age children in their villages. The ComDT approach was well received and was successfully implemented in the villages. Although the villagers pointed out the limitation in reaching the non-enrolled children in the school-based approach, they also expressed satisfaction with this approach. This study suggests that the ComDT approach is well accepted and can be implemented effectively to ensure better coverage of especially non-enrolled school-age children. 相似文献
100.
Hillary S.W. Han 《Mathematical biosciences》2009,219(1):7-14
In this paper we study the distribution of stacks/loops in k-non-crossing, τ-canonical RNA pseudoknot structures (〈k,τ〉-structures). Here, an RNA structure is called k-non-crossing if it has no more than k-1 mutually crossing arcs and τ-canonical if each arc is contained in a stack of length at least τ. Based on the ordinary generating function of 〈k,τ〉-structures [G. Ma, C.M. Reidys, Canonical RNA pseudoknot structures, J. Comput. Biol. 15 (10) (2008) 1257] we derive the bivariate generating function , where Tk,τ(n,t) is the number of 〈k,τ〉-structures having exactly t stacks and study its singularities. We show that for a specific parametrization of the variable u, Tk,τ(x,u) exhibits a unique, dominant singularity. The particular shift of this singularity parametrized by u implies a central limit theorem for the distribution of stack-numbers. Our results are of importance for understanding the ‘language’ of minimum-free energy RNA pseudoknot structures, generated by computer folding algorithms. 相似文献