全文获取类型
收费全文 | 216篇 |
免费 | 23篇 |
国内免费 | 9篇 |
专业分类
248篇 |
出版年
2023年 | 2篇 |
2021年 | 2篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 9篇 |
2013年 | 13篇 |
2012年 | 6篇 |
2011年 | 24篇 |
2010年 | 4篇 |
2009年 | 7篇 |
2008年 | 12篇 |
2007年 | 8篇 |
2006年 | 9篇 |
2005年 | 4篇 |
2004年 | 9篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1995年 | 3篇 |
1992年 | 2篇 |
1991年 | 8篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1971年 | 2篇 |
1970年 | 4篇 |
1969年 | 2篇 |
1968年 | 3篇 |
1967年 | 2篇 |
1966年 | 4篇 |
1965年 | 2篇 |
1964年 | 2篇 |
1957年 | 3篇 |
1955年 | 1篇 |
1953年 | 1篇 |
1928年 | 1篇 |
排序方式: 共有248条查询结果,搜索用时 0 毫秒
41.
42.
43.
44.
45.
46.
The recently determined crystal structures of two aspartic proteinase zymogens, prophytepsin from barley and proplasmepsin II from the malarial parasite Plasmodium falciparum, have provided new insights into zymogen inactivation. Prophytepsin shows a variation of the mechanism of inhibition used by the well-known gastric aspartic proteinase zymogens, whereas proplasmepsin II uses a completely new mode of inactivation. 相似文献
47.
S.P. NG Z.G. LI B.W. CHEN Z.K. QIN M.M. GARCIA S.W.K. IM M.H. NG 《Journal of Rapid Methods and Automation in Microbiology》1997,5(4):285-294
We previously described an enrichment-immunoassay utilizing a T6 monoclonal antibody capture enzyme-linked immunosorbent assay. Here we evaluated it for the rapid screening for Salmonella in fishmeal obtained from the national Animal and Plant Quarantine service in the People's Republic of China. In this method, the number of Salmonella present is first expanded by appropriate enrichment cultures, and the pathogens are then directly detected by the T6 immunoassay. In a total of 94 enrichment cultures of fishmeal, we obtained an overall concordance of 98% between the results obtained in parallel by this method and by conventional test method. The positive prediction by this method was 92% and the negative prediction was 100%. The turn around time for the new test was 27 h which is a significant improvement from the turn around time exceeding 96 h required for the conventional test method. This test proved to be compatible with the routine work flow in the practical setting of a quarantine laboratory. 相似文献
48.
49.
Kitabwalla M Ansari AA Villinger F Akeefe H Conner AP Mayne AE Hildreth JE 《Experimental biology and medicine (Maywood, N.J.)》2008,233(6):732-740
This pilot experiment in a simian immunodeficiency virus (SIV) chronic infection model aimed at extending our previous findings that vaccination with delipidated SIV resulted in more potent and diversified antiviral responses (1). Macaques chronically infected with SIVmac239 treated with antiretroviral therapy (ART) were vaccinated with autologous delipidated virus via consecutive lymph node targeted immunizations-1, 1 and 10 mug of virus spaced monthly. Results showed all animals had lasting viral load reduction approaching 1 log compared to set-point, and disease delay. Delipidation may enhance processing/ presentation of viral antigen eliciting potent antiviral control even at such late infection stage. 相似文献
50.