排序方式: 共有119条查询结果,搜索用时 31 毫秒
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Lucas SJ Šimková H Šafář J Jurman I Cattonaro F Vautrin S Bellec A Berges H Doležel J Budak H 《Functional & integrative genomics》2012,12(1):173-182
Bread wheat (Triticum aestivum L.) is one of the most important crops globally and a high priority for genetic improvement, but its large and complex genome
has been seen as intractable to whole genome sequencing. Isolation of individual wheat chromosome arms has facilitated large-scale
sequence analyses. However, so far there is no such survey of sequences from the A genome of wheat. Greater understanding
of an A chromosome could facilitate wheat improvement and future sequencing of the entire genome. We have constructed BAC
library from the long arm of T. aestivum chromosome 1A (1AL) and obtained BAC end sequences from 7,470 clones encompassing the arm. We obtained 13,445 (89.99%) useful
sequences with a cumulative length of 7.57 Mb, representing 1.43% of 1AL and about 0.14% of the entire A genome. The GC content
of the sequences was 44.7%, and 90% of the chromosome was estimated to comprise repeat sequences, while just over 1% encoded
expressed genes. From the sequence data, we identified a large number of sites suitable for development of molecular markers
(362 SSR and 6,948 ISBP) which will have utility for mapping this chromosome and for marker assisted breeding. From 44 putative
ISBP markers tested 23 (52.3%) were found to be useful. The BAC end sequence data also enabled the identification of genes
and syntenic blocks specific to chromosome 1AL, suggesting regions of particular functional interest and targets for future
research. 相似文献
93.
Autophagy-related gene, TdAtg8, in wild emmer wheat plays a role in drought and osmotic stress response 总被引:1,自引:0,他引:1
Kuzuoglu-Ozturk D Cebeci Yalcinkaya O Akpinar BA Mitou G Korkmaz G Gozuacik D Budak H 《Planta》2012,236(4):1081-1092
An autophagy-related gene Atg8 was cloned for the first time from wild emmer wheat, named as TdAtg8, and its role on autophagy under abiotic stress conditions was investigated. Examination of TdAtg8 expression patterns indicated that Atg8 expression was strongly upregulated under drought stress, especially in the roots when compared to leaves. LysoTracker(?) red marker, utilized to observe autophagosomes, revealed that autophagy is constitutively active in Triticum dicoccoides. Moreover, autophagy was determined to be induced in plants exposed to osmotic stress when compared to plants grown under normal conditions. Functional studies were executed in yeast to confirm that the TdATG8 protein is functional, and showed that the TdAtg8 gene complements the atg8?::kan MX yeast mutant strain grown under nitrogen deficiency. For further functional analysis, TdATG8 protein was expressed in yeast and analyzed using Western immunoblotting. Atg8-silenced plants were exposed to drought stress and chlorophyll and malondialdehyde (MDA) content measurements demonstrated that Atg8 plays a key role on drought stress tolerance. In addition, Atg8-silenced plants exposed to osmotic stress were found to have decreased Atg8 expression level in comparison to controls. Hence, Atg8 is a positive regulator in osmotic and drought stress response. 相似文献
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Saçmacı Hikmet Tanık Nermin Ökçesiz İzzet İntepe Yavuz Selim Aktürk Tülin Çiftçi Bülent İnan Levent Ertuğrul 《Sleep and biological rhythms》2019,17(4):433-439
Sleep and Biological Rhythms - The relationship between sleep and brain lesions remains unknown in patients with multiple sclerosis (MS) and is currently under investigation. Sleep deprivation and... 相似文献
96.
Srinivas Reddy Chirapu Boobalan Pachaiyappan Hikmet F. Nural Xin Cheng Hongbin Yuan David C. Lankin Samer O. Abdul-Hay Gregory R.J. Thatcher Yong Shen Alan P. Kozikowski Pavel A. Petukhov 《Bioorganic & medicinal chemistry letters》2009,19(1):264-274
A series of transition state analogues of beta-secretases 1 and 2 (BACE1, 2) inhibitors containing fused-ring or biaryl moieties were designed computationally to probe the S2 pocket, synthesized, and tested for BACE1 and BACE2 inhibitory activity. It has been shown that unlike the biaryl analogs, the fused-ring moiety is successfully accommodated in the BACE1 binding site resulting in the ligands with excellent inhibitory activity. Ligand 5b reduced 65% of Aβ40 production in N2a cells stably transfected with Swedish human APP. 相似文献
97.
Hikmet Birand 《Plant Ecology》1970,20(1-4):21-47
Zusammenfassung Die trockenste Region in der zentralanatolischen Steppe ist das Karapinar-Gebiet. Die klimatischen Verhältnisse dieses Gebietes wurden vergleichend erörtert. Bodenprofile der zwei für dieses Gebiet charakteristischen Pflanzengesellschaften wurden auch nach ihrer chemischen und physikalischen Beschaffenheit untersucht. Die Pflanzengesellschaft der Sandwüste wurde beschrieben. Die weitaus vorherrschende und wohl auch ursprüngliche Pflanzengesellschaft in diesem Gebiet ist dieArtemisia fragrans-Steppe. Sie ist durch Überweidung stark degradiert und befindet sich in Umwandlung zur Wüste. Ursache und Verlauf der Verwüstung in jüngster Zeit wurde eingehend geschildert.
Summary The driest region in the central anatolian steppe (Turkey) is the area around the town Karapinar. The climate of this area is compared to others in central Anatolia. Soil profiles for two plant associations, characteristic for this area, are investigated as to their chemical and physical properties.The author describes the plant association of the sand desert. The dominant and probably original plant association in this area is theArtemisia fragrans-Steppe. It is very much degraded by intensive sheep grazing, and is being transformed into desert.Factors involved in this process in recent times are dealt with.相似文献
98.
Bala Ani Akpinar Federica Magni Meral Yuce Stuart J. Lucas Hana ?imková Jan ?afá? Sonia Vautrin Hélène Bergès Federica Cattonaro Jaroslav Dole?el Hikmet Budak 《BMC genomics》2015,16(1)
Background
The substantially large bread wheat genome, organized into highly similar three sub-genomes, renders genomic research challenging. The construction of BAC-based physical maps of individual chromosomes reduces the complexity of this allohexaploid genome, enables elucidation of gene space and evolutionary relationships, provides tools for map-based cloning, and serves as a framework for reference sequencing efforts. In this study, we constructed the first comprehensive physical map of wheat chromosome arm 5DS, thereby exploring its gene space organization and evolution.Results
The physical map of 5DS was comprised of 164 contigs, of which 45 were organized into 21 supercontigs, covering 176 Mb with an N50 value of 2,173 kb. Fifty-eight of the contigs were larger than 1 Mb, with the largest contig spanning 6,649 kb. A total of 1,864 molecular markers were assigned to the map at a density of 10.5 markers/Mb, anchoring 100 of the 120 contigs (>5 clones) that constitute ~95 % of the cumulative length of the map. Ordering of 80 contigs along the deletion bins of chromosome arm 5DS revealed small-scale breaks in syntenic blocks. Analysis of the gene space of 5DS suggested an increasing gradient of genes organized in islands towards the telomere, with the highest gene density of 5.17 genes/Mb in the 0.67-0.78 deletion bin, 1.4 to 1.6 times that of all other bins.Conclusions
Here, we provide a chromosome-specific view into the organization and evolution of the D genome of bread wheat, in comparison to one of its ancestors, revealing recent genome rearrangements. The high-quality physical map constructed in this study paves the way for the assembly of a reference sequence, from which breeding efforts will greatly benefit.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1641-y) contains supplementary material, which is available to authorized users. 相似文献99.
Kim Dongjin Hager Megan Brant Eleanor Budak Hikmet 《Functional & integrative genomics》2021,21(3-4):355-366
Functional & Integrative Genomics - Genome editing can be used to create new wheat varieties with enhanced performance. Clustered regularly interspaced short palindromic repeat (CRISPR) is a... 相似文献
100.
Expression and Cellular Localization of ZIP1 Transporter Under Zinc Deficiency in Wild Emmer Wheat 总被引:1,自引:0,他引:1