全文获取类型
收费全文 | 101篇 |
免费 | 10篇 |
出版年
2021年 | 2篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2016年 | 3篇 |
2015年 | 5篇 |
2014年 | 2篇 |
2013年 | 2篇 |
2012年 | 5篇 |
2011年 | 3篇 |
2010年 | 3篇 |
2009年 | 4篇 |
2008年 | 4篇 |
2007年 | 3篇 |
2006年 | 3篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2003年 | 5篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 5篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 7篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有111条查询结果,搜索用时 31 毫秒
91.
Caygill RL Hodges CS Holmes JL Higson SP Blair GE Millner PA 《Biosensors & bioelectronics》2012,32(1):104-110
The number of Adenovirus (Ad) infections detected in immunocompromised people has increased due to the number of patients receiving transplants, as well as the HIV pandemic. Ads cause life-threatening diseases specific to the infected organs of immunocompromised hosts, with discontinuation of immunosuppressive agents necessary to prevent morbidity. The methodology in this paper has been employed to develop a novel impedimetric based assay platform to detect and quantify human Ads, which is comparable in performance to current methods, such as ELISA and PCR, but is also less expensive and faster. Novel immunosensors have been fabricated using polyclonal antibodies raised against a human Ad (Ad5) capsid protein, which were selectively cleaved into antibody fragments by 2-mercaptoethylamine. The fragments were immobilized onto a functionalized conducting copolymer matrix comprising polyaniline and 2-aminobenzylamine. Fully fabricated sensors were incubated with two immunologically distinct serotypes of Ad, Ad5 and Ad3, with between 10 and 10(12)virus particles/mL prior to sensor interrogation. Electrochemical impedance spectroscopy was used to measure the charge transfer resistance of the sensors over a range of frequencies from 25 kHz to 0.1 Hz. Our data demonstrate that the immunosensors specifically detect, and differentiate between, closely related human Ad serotypes with a limit of detection of 10(3)virus particles/mL. In addition, atomic force microscopy was applied to study the sensor surface nanostructure. Future work looks to test virus containing clinical samples but this could be a viable and valuable alternative for point-of-care virus detection and quantification. 相似文献
92.
St John JA Braun EL Isberg SR Miles LG Chong AY Gongora J Dalzell P Moran C Bed'hom B Abzhanov A Burgess SC Cooksey AM Castoe TA Crawford NG Densmore LD Drew JC Edwards SV Faircloth BC Fujita MK Greenwold MJ Hoffmann FG Howard JM Iguchi T Janes DE Khan SY Kohno S de Koning AJ Lance SL McCarthy FM McCormack JE Merchant ME Peterson DG Pollock DD Pourmand N Raney BJ Roessler KA Sanford JR Sawyer RH Schmidt CJ Triplett EW Tuberville TD Venegas-Anaya M Howard JT Jarvis ED Guillette LJ Glenn TC 《Genome biology》2012,13(1):415-12
The International Crocodilian Genomes Working Group (ICGWG) will sequence and assemble the American alligator (Alligator mississippiensis), saltwater crocodile (Crocodylus porosus) and Indian gharial (Gavialis gangeticus) genomes. The status of these projects and our planned analyses are described. 相似文献
93.
The adsorption of genomic DNA and subsequent interactions between adsorbed and solvated DNA was studied using a novel sensitive optical method of total internal reflection ellipsometry (TIRE), which combines spectroscopic ellipsometry with surface plasmon resonance (SPR). Single strands of DNA of two species of fish (herring and salmon) were electrostatically adsorbed on top of polyethylenimine films deposited upon gold coated glass slides. The ellipsometric spectra were recorded and data fitting utilized to extract optical parameters (thickness and refractive index) of adsorbed DNA layers. The further adsorption of single stranded DNA from an identical source, i.e. herring ss-DNA on herring ss-DNA or salmon ss-DNA on salmon ss-DNA, on the surface was observed to give rise to substantial film thickness increases at the surface of about 20-21 nm. Conversely adsorption of DNA from alternate species, i.e. salmon ss-DNA on herring ss-DNA or herring ss-DNA on salmon ss-DNA, yielded much smaller changes in thickness of 3-5 nm. AFM studies of the surface roughness of adsorbed layers were in line with the TIRE data. 相似文献
94.
Simultaneous saccharification and fermentation of straw to ethanol using the thermotolerant yeast strain Kluyveromyces marxianus imb3 总被引:1,自引:0,他引:1
The thermotolerant, ethanol-producing yeast strain Kluyveromyces marxianus IMB3 was grown at 45°C on media containing 2, 4 and 6 % (w/v) pulverized barley straw and supplemented with 2% (v/v) cellulase. Maximum ethanol concentrations produced were 2, 3 and 3.6g/l, respectively. When the pulverized straw was replaced by NaOH pretreated straw (at 2, 4 and 6% (w/v); based on original untreated straw), ethanol concentrations increased to maxima of 3.9, 8, and 12g/l, respectively. The ethanol yields amount to 20g ethanol from 100g of straw. 相似文献
95.
Phylogenetic analysis of slippage-like sequence variation in the V4 rRNA expansion segment in tiger beetles (Cicindelidae) 总被引:1,自引:1,他引:0
Sequence variation in the middle part of the small-subunit rRNA was studied
for representatives of the major groups in the family Cicindelidae
(Coleoptera). All taxa exhibited a much expanded segment in variable region
V4 compared to D. melanogaster. This expanded segment was not found in
other groups of beetles, including three taxa in the closely related
Carabidae. Secondary structure predictions indicate that the expanded
segment folds into a single stem-loop structure in all taxa. Despite its
structural conservation, the fragment differs strongly in primary sequence,
even between closely related sister taxa. Several features of these
sequences are consistent with slippage replication as the mechanism that
has generated this sequence variation: the level of internal sequence
repetition as measured by the relative simplicity factor (RSF), its
variation in length between close relatives, and the strong nucleotide bias
compared to the remainder of the gene. With few exceptions, there was also
a correlation between sequence length and the level of sequence repetition,
frequently interpreted as the result of slippage. Phylogenies inferred from
the expansion segment were not consistent with existing hypotheses from
other molecular data for the group. This indicates that DNA sequences in
this region are not homologous throughout the entire Cicindelidae, but it
leaves open the possibility that this expansion segment can be used for
phylogeny reconstruction within subgroups. The implications of a
phylogenetic approach to the understanding of slippage-like evolution are
discussed.
相似文献
96.
B S Hernandez F K Higson R Kondrat D D Focht 《Applied and environmental microbiology》1991,57(11):3361-3366
Pseudomonas putida P111 was isolated by enrichment culture on 2,5-dichlorobenzoate and was also able to grow on 2-chloro-, 3-chloro-, 4-chloro-, 2,3-dichloro-, 2,4-dichloro-, and 2,3,5-trichlorobenzoates. However, 3,5-dichlorobenzoate completely inhibited growth of P111 on all ortho-substituted benzoates that were tested. When 3,5-dichlorobenzoate was added as a cosubstrate with either 3- or 4-chlorobenzoate, cell yields and chloride release were greater than those observed from growth on either monochlorobenzoate alone. Moreover, resting cells of P111 grown on 4-chlorobenzoate released chloride from 3,5-dichlorobenzoate and produced no identifiable intermediate. In contrast, resting cells grown on 2,5-dichlorobenzoate metabolized 3,5-dichlorobenzoate without release of chloride and accumulated a degradation product, which was identified as 1-carboxy-1,2-dihydroxy-3,5-dichlorocyclohexadiene on the basis of gas chromatography-mass spectrometry confirmation of its two acid-hydrolyzed products, 3,5- and 2,4-dichlorophenol. Since 3,5-dichlorocatechol was rapidly metabolized by cells grown on 2,5-dichlorobenzoate, it is apparent that 1-carboxy-1,2-dihydroxy-3,5-dichlorocyclohexadiene is not further metabolized by these cells. Moreover, induction of a functional dihyrodiol dehydrogenase would not be required for growth of P111 on other ortho-chlorobenzoates since the corresponding chlorodihydrodiols produced from a 1,2-dioxygenase attack would spontaneously decompose to the corresponding catechols. In contrast, growth on 3-chloro-, 4-chloro-, or 3,5-dichlorobenzoate requires a functional dihydrodiol dehydrogenase, yet only the two monochlorobenzoates appear to induce for it. 相似文献
97.
Isolates able to grow on 3- or 4-hydroxybiphenyl (HB) as the sole carbon source were obtained by enrichment culture. The 3-HB degrader Pseudomonas sp. strain FH12 used an NADPH-dependent monooxygenase restricted to 3- and 3,3'-HBs to introduce an ortho-hydroxyl. The 4-HB degrader Pseudomonas sp. strain FH23 used either a mono- or dioxygenase to generate a 2,3-diphenolic substitution pattern which allowed meta-fission of the aromatic ring. By using 3-chlorocatechol to inhibit catechol dioxygenase activity, it was found that 2- and 3-HBs were converted by FH23 to 2,3-HB, whereas biphenyl and 4-HB were attacked by dioxygenation. 4-HB was metabolized to 2,3,4'-trihydroxybiphenyl. Neither organism attacked chlorinated HBs. The degradation of 3- and 4-HBs by these strains is therefore analogous to the metabolism of biphenyl, 2-HB, and naphthalene in the requirement for 2,3-catechol formation. 相似文献
98.
Evolutionary relatedness of some primate models of Plasmodium 总被引:1,自引:0,他引:1
Primate--and, specifically, monkey--malaria infections are commonly used
for understanding the pathology of and immune response to the human disease
because they are thought to resemble most closely the host-parasite
relationship found in humans. Plasmodium cynomolgi is used extensively as a
model for the human parasite, P. vivax, and P. knowlesi is used primarily
as a model for the development of erythrocytic-stage vaccines. Both of
these simian parasites can naturally infect man, resulting in mildly
symptomatic episodes of the disease. The phylogenetic relationship between
these two simian parasites and previously characterized Plasmodium species,
including P. vivax, was examined by comparison of the asexually expressed
small- subunit ribosomal RNA genes. Our analysis confirmed that P. vivax is
most closely related to P. cynomolgi and that it remains an appropriate
model of the human pathogen. Furthermore, with P. knowlesi and P. fragile,
these two species form a group of closely related species, distant from
other Plasmodium species. What is considered to be the most ancient of the
human malaria pathogens, P. malariae, was also included in the analysis and
does not group at all with other simian or human parasites.
相似文献
99.
Evolution and phylogenetic information content of the ITS-1 region in the tiger beetle Cicindela dorsalis 总被引:6,自引:0,他引:6
Sequence divergence in the internal transcribed spacer region 1 (ITS-1) of
the ribosomal DNA locus was assessed in subspecies of the coastal North
American tiger beetle, Cicindela dorsalis. The spacer region was amplified
using the polymerase chain reaction and cloned for sequencing. Of a total
of 50 clones obtained from 12 specimens, 42 clones were different in at
least one nucleotide position. In a parsimony analysis of these sequences,
the main phylogenetic distinction was found to separate sequences from the
Gulf of Mexico and the Atlantic Ocean. Within these two assemblages
phylogenetic resolution was low, and the variation within individuals was
almost as high as the variation within the entire lineage. The pattern of
sequence variation suggests the existence of two forms of the ITS-1 that
are maintained on different chromosomes. Polymorphisms of limited
geographical distribution could be detected, and 41 additional clones were
partly sequenced, to assess the geographic distribution of these
polymorphisms in more detail. In a population aggregation analysis, the
geographic pattern of ITS-1 distribution was basically congruent with that
obtained in earlier studies from mitochondrial DNA in the same C. dorsalis
populations.
相似文献
100.
Electrochemical impedance spectroscopy (EIS) is a widely used technique for probing bioaffinity interactions at the surfaces of electrically conducting polymers. EIS methods can be employed to investigate 'labeless' detection of analytes via impedimetric transduction. This paper describes the development of a direct immunosensor for the detection of a cell-surface protein on Listeria monocytogenes, an extremely important food-borne pathogen. L. monocytogenes are facultative anaerobic, non-sporing, Gram-positive, motile rods that employ the surface bound protein, Internalin B (InlB), to promote invasion into host cells. A recombinant form of InlB was previously cloned and expressed in Escherichia coli and a panel of antibodies and antibody fragments directed against the protein were also produced. Here, we describe how a portion of the recombinant InlB protein, the F3 fragment, and an anti-InlB polyclonal antibody, were used to develop a platform for the labeless immunosensing of InlB. Sensors were fabricated by electropolymerisation of planar screen-printed carbon electrodes with polyaniline (PANI), to produce a conductive substrate. Polyclonal anti-InlB antibody was subsequently incorporated onto the PANI layer using a biotin-avidin system for site-specific immobilisation. The sensors were then probed with varying concentrations of InlB antigen and the impedimetric response at each concentration was recorded. An anti-IgG antibody was immobilised at the electrode surface, as a control and subsequently exposed to the same concentrations of InlB. Impedimetric data for the control sensors were also recorded. Upon exposure to a range of concentrations of antigen, complex plane impedance analyses were used to relate the differing redox states of the polymer layer, to the possible charge transfer at the surface, with respect to the related mechanisms between the antibody and the polymer. These effects were subsequently monitored to assess the impedance of the polymer thereby determining the amount of bound antigen at the sensor surface. Calibration profiles for both sample (InlB) and control (IgG) sensors were constructed. A limit of detection of 4.1 pg/ml was achieved for Internalin B. 相似文献