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91.
Macedo TB Bastos CS Higley LG Ostlie KR Madhavan S 《Journal of economic entomology》2003,96(1):188-193
The soybean aphid, Aphis glycines Matsumara, was discovered in the United States in the summer of 2000. Since that initial discovery, the aphid has spread across northern soybean production regions. In 2001, we examined the physiological responses of soybeans to low aphids densities (fewer than 50 aphids/leaf). In this study, we determined photosynthetic rates, leaf fluorescence responses, and photosynthetic responses to variable carbon dioxide and light levels. In addition, analyses for chlorophyll content and stable carbon isotope ratios were used to differentiate potential differences in stomatal versus mesophyll limitations to photosynthesis. We observed rate reductions of up to 50% on infested leaflets, including lealets with no apparent symptoms of aphid injury (such as chlorosis). Differences in fluorescence data indicated that photoelectron transport was not impaired. These results indicate that substantial physiological impact on soybean is possible even at low aphid densities. Also, the conventional view of aphid injury acting through reductions in chlorophyll content and light-harvesting reactions of photosynthesis is not supported by our findings in this system. 相似文献
92.
Economic injury levels and sequential sampling plans for Mexican bean beetle (Coleoptera: Coccinellidae) on dry beans 总被引:1,自引:0,他引:1
Field studies were conducted during the growing seasons of 1995 and 1996, in Scotts-bluff, Nebraska, to determine yield-loss relationships for Mexican bean beetle (Epilachna varivestis Mulsant) on dry bean (Phaseolus vulgaris L.). Results of those experiments were combined with data from other studies previously conducted to develop economic injury levels (EILs), economic thresholds (ETs), and a sequential sampling program for Mexican bean beetle. Yield loss was regressed against larvae/row-m, and the slope of the linear regression (113 kg/ha per larvae/row-m) was used as the DI (yield loss/insect density) variable in EIL calculations. The EILs calculated in larvae/row-m were converted to egg masses/row-m and adjusted to reflect average survivorship to the adult stage. An example EIL for esfenvalerate at 0.509 (formulation) liter/ha (0.0453 gal/a) and crop value of 0.44 dollars/kg (20 dollars/100 lbs) was 17.78 larvae/row-m. The corresponding ET is 1.04 egg masses/row-m, which reflects an average of 54.6 eggs/egg mass and 33% survival rate from egg to injurious stages. Sequential sampling plans were calculated based on a negative binomial distribution using parameter k estimated from previous research. Because sampling is based on egg masses, growers can make management decisions and take management actions before significant injury occurs. Also, ETs can be adjusted to include the occurrence of natural mortality in the egg and early instars. Analyses demonstrated that relatively minor variation in ETs has substantial impact on sequential sampling plans, including parameters such as average sample number. An interactive spreadsheet was developed that allows users to input economic and other data specific to their situation to calculate Mexican bean beetle EILs, ETs, and sequential sampling plans. 相似文献
93.
W. Wyatt Hoback Thomas L. Clark Lance J. Meinke Leon G. Higley Joanne M. Scalzitti 《Entomologia Experimentalis et Applicata》2002,105(1):29-34
Soil dwelling invertebrates including insects and their larvae are subjected to severe oxygen limitations when the soil becomes saturated or covered by water. Differential survival of this stress may in part explain ecological range of a species and could lead to cultural control methods for economically important species. We tested immersion survival for larvae of three species of Diabrotica (viz., D. balteata LeConte, D. undecimpunctata undecimpunctata Mannerheim, and D. virgifera virgifera LeConte). Groups of larvae were submersed in conditioned, hypoxic (dissolved oxygen <0.3 ppm) tap water, held at 10, 15, 20, or 25 °C, and periodically removed and assayed for survivorship. We found that time to 50% mortality (LT50) differed significantly between species. Third instar D. u. undecimpunctata were most sensitive to immersion at 25 °C (LT50=9 h), D. balteata were intermediate (LT50=15 h) and D. v. virgifera larvae were least sensitive (LT50=23 h). Second instar D. v. virgifera were significantly more tolerant of immersion than the other species (LT50=56 h versus 15 h for D. u. undecimpunctata and 11 h for D. balteata). Mortality during immersion corresponds with the build up of lactic acid. Survivorship for all species increased with decreasing temperature. The use of flooding in rootworm management is briefly discussed. 相似文献
94.
95.
Interferon alpha (IFNA) genes code for proteins with important signaling roles during the innate immune response. Phylogenetically, IFNA family members in eutherians (placental mammals) cluster together in a species-specific manner except for closely related species (i.e. Homo sapiens and Pan troglodytes) where gene-specific clustering is evident. Previous research has been unable to clarify whether gene conversion or recent gene duplication accounts for gene-specific clustering, partly because the similarity of members of the IFNA family within species has made it historically difficult to identify the exact composition of IFNA gene families. IFNA gene families were fully characterized in recently available genomes from Canis familiaris, Macaca mulatta, P. troglodytes and Rattus norvegicus, and combined with previously characterized IFNA gene families from H. sapiens and Mus musculus, for the analysis of both whole and partial gene conversion events using a variety of statistical methods. Gene conversion was inferred in every eutherian species analyzed and comparison of the IFNA gene family locus between primate species revealed independent gene duplication in M. mulatta. Thus, both gene conversion and gene duplication have shaped the evolution of the IFNA gene family in eutherian species. Scenarios may be envisaged whereby the increased production of a specific IFN-alpha protein would be beneficial against a particular pathogenic infection. Gene conversion, similar to duplication, provides a mechanism by which the protein product of a specific IFNA gene can be increased. 相似文献
96.
Background
Inteins are self-splicing protein elements. They are translated as inserts within host proteins that excise themselves and ligate the flanking portions of the host protein (exteins) with a peptide bond. They are encoded as in-frame insertions within the genes for the host proteins. Inteins are found in all three domains of life and in viruses, but have a very sporadic distribution. Only a small number of intein coding sequences have been identified in eukaryotic nuclear genes, and all of these are from ascomycete or basidiomycete fungi. 相似文献97.
Anderson WG Heng-Moss TM Baxendale FP Baird LM Sarath G Higley L 《Journal of economic entomology》2006,99(1):212-221
Chinch bugs are common pests of many agronomic and horticulturally important crops and turfgrasses. Previous research has indicated that some grasses exhibit resistance to multiple chinch bug species, whereas others are resistant to only one species. The objectives of this research were to document differences in the probing frequencies and locations among Blissus species as well as differences in mouthpart morphology as a first step in understanding the differential responses of grasses to chinch bug feeding. Scanning electron microscopy detected differences in the total lengths of proboscises as well as individual mouthpart segments among the four species studied. Blissus occiduus Barber probed significantly more often on buffalograss, Buchlo? dactyloides (Nuttall) Engelmann, than any other plant material. Probing locations of B. occiduus and Blissus leucopterus leucopterus (Say) were similar on both B. occiduus-resistant and susceptible buffalograsses and KS94 sorghum, Sorghum bicolor (L.) Moench (B. occiduus-resistant, B. l. leucopterus-resistant). However, on 'Wheatland' sorghum (B. occiduus-resistant, B. l. leucopterus-susceptible), stylet tracts of B. l. leucopterus most often terminated in the bundle sheath cells, whereas those of B. occiduus generally terminated in the vascular tissues. 相似文献
98.
Relationship between platelet-activating factor concentration in rhesus monkey (Macaca mulatta) spermatozoa and sperm motility. 总被引:1,自引:0,他引:1
William E Roudebush Melissa S Gerald Jose A Cano Isabelle D Lussier Greg Westergaard J Dee Higley 《American journal of primatology》2002,56(1):1-7
Platelet-activating factor (PAF) is a potent signaling phospholipid that has been implicated in a number of biological activities. PAF concentration in primate spermatozoa has a positive correlation with fertility. While PAF is present in rhesus spermatozoa, there are no relational reports on its concentration and the cell's motility. The study objective was to determine if PAF concentration in rhesus spermatozoa was correlated with motility indices (percent motility and forward progression). Semen was collected from sexually mature males and cell counts, and percent motilities and forward progressions were recorded prior to PAF measurement by radioimmunoassay. Spermatozoa-derived PAF concentration ranged from a low of 0.9 picomoles/10(6) cells to a high of 13.0 picomoles/10(6) cells. The overall mean (+/-SEM) PAF concentration was 4.6 (+/-1.6) picomoles/10(6) spermatozoa. Regression analysis revealed a positive and significant relationship between PAF concentration in the spermatozoa and percent motility (R2 = 0.914; P < 0.01) as well as forward progression (R2 = 0.849; P < 0.05). A receiver-operator characteristic curve and the calculation of the probability that a positive forward progression will be predicted indicated a cutoff limit of 1.5 picomoles/10(6) cells for PAF concentration in rhesus sperm. Rhesus monkey spermatozoa motility was significantly greater (P < 0.01) in the high-PAF (> or =2 picomoles/10(6) cells) group (31.0 +/- 7.6) than in the low-PAF (<2 picomoles/10(6) cells) group (6.8 +/- 2.1). Rhesus monkey spermatozoa forward progression was significantly greater (P < 0.05) in the high-PAF (> or =2 picomoles/10(6) cells) group (3.0 +/- 1.0) than in the low-PAF (<2 picomoles/10(6) cells) group (0.7 +/- 0.3). The data demonstrate that PAF concentration in rhesus spermatozoa has a significant relationship with percent motility and the cell's forward progression. Determining PAF concentration in spermatozoa may be a significant predictor of fertility in the primate. Additional studies will elucidate the role of PAF in spermatozoa function and the significance PAF plays in primate fertility. 相似文献
99.
J. Dee Higley 《American journal of primatology》1984,6(3):253-254
100.
Kathryn A. Higley 《Radiation and environmental biophysics》2010,49(4):645-656
The calculation of transfer of radionuclides from the abiotic to the biotic environment is a well-established practice in
radiological assessments. Concentration ratios provide simple means to estimate radionuclide activity in biota, from measured
(or estimated) radionuclide concentrations in either a food source or an abiotic component such as soil or water. They are
typically reported by element, and data compilations may include information such as soil type (e.g., sand, loam, clay) and
species. The data may be for multiple species at a single location, single species at multiple locations, or represent compilations
from multiple sources. Recently published guidance suggests that estimates are best made using data from the same ecosystem.
This paper examines this recent guidance, in the context of using measured data from within a single ecosystem and comparing
results to more generic values. Results suggest that generic values may be an adequate substitute for site-specific information.
It illustrates how ionic potential may be used as an alternative to group chemical properties in estimating transfer factors.
Lastly, limited evidence is found to support the concept of allometric scaling functions for elemental concentrations in plants. 相似文献