全文获取类型
收费全文 | 798篇 |
免费 | 56篇 |
专业分类
854篇 |
出版年
2022年 | 6篇 |
2021年 | 9篇 |
2019年 | 5篇 |
2018年 | 11篇 |
2017年 | 11篇 |
2016年 | 11篇 |
2015年 | 22篇 |
2014年 | 26篇 |
2013年 | 36篇 |
2012年 | 39篇 |
2011年 | 32篇 |
2010年 | 27篇 |
2009年 | 25篇 |
2008年 | 38篇 |
2007年 | 27篇 |
2006年 | 38篇 |
2005年 | 34篇 |
2004年 | 38篇 |
2003年 | 27篇 |
2002年 | 27篇 |
2001年 | 22篇 |
2000年 | 26篇 |
1999年 | 15篇 |
1998年 | 13篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 20篇 |
1991年 | 16篇 |
1990年 | 28篇 |
1989年 | 13篇 |
1988年 | 22篇 |
1987年 | 11篇 |
1986年 | 11篇 |
1985年 | 15篇 |
1984年 | 11篇 |
1983年 | 12篇 |
1982年 | 7篇 |
1981年 | 5篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1975年 | 6篇 |
1974年 | 9篇 |
1973年 | 7篇 |
1972年 | 7篇 |
1971年 | 6篇 |
1970年 | 13篇 |
1968年 | 4篇 |
1967年 | 4篇 |
排序方式: 共有854条查询结果,搜索用时 15 毫秒
851.
852.
A new method for purification of anti-glycosphingolipid antibody. Avian anti-hematoside (NeuGc) antibody 总被引:6,自引:0,他引:6
Y Hirabayashi T Suzuki Y Suzuki T Taki M Matsumoto H Higashi S Kato 《Journal of biochemistry》1983,94(1):327-330
A new method for purification of anti-glycosphingolipid antibodies had been developed. N-Glycolylneuraminyl(alpha 2-3)lactosylceramide [hematoside (NeuGc)] could be hydrophobically bound on octyl-Sepharose 4B in the presence of 0.1 M KCl. The Sepharose gel coated with hematoside (NeuGc) was used as immunoadsorbent for affinity column chromatography to purify avian anti-hematoside (NeuGc) antibody. The procedure is very simple, reproducible and applicable to purification of almost all anti-glycosphingolipid antibodies. The glycosphingolipid used for the affinity chromatography could be recovered without any destruction by successive extraction of the gel with methanol and methanol/chloroform (1:2, v/v). 相似文献
853.
Two-dimensional electrophoretograms of extracts of [3H]glucosamine-labeled human renal cancer cells demonstrated a series of components (Mr 48,000 and 30,000) that are only poorly expressed in similarly labeled normal kidney epithelial cell cultures [S. Ogata, R. Ueda, and K. O. Lloyd (1981) Proc. Natl. Acad Sci. USA 78, 770-774]. These characteristics are also exhibited by [3H]Man-labeled samples and by concanavalin A-binding glycoproteins from [35S]Met-labeled cells. It is now shown that these species are the precursor chain (Mr 48,000) and native heavy chain (Mr 30,000) forms of the lysosomal enzyme, cathepsin D. These results were obtained by precipitation with a specific anti-cathepsin D serum and by binding of the components to pepstatin-Sepharose. Cathepsin D heavy chain is heterogeneous, having three major species with pI's of 5.7, 5.3, and 4.9; all forms are glycosylated with high mannose-type chains [approximate size: Man5(GlcNAc)2] and are partially phosphorylated. Despite these indications of dissimilarities in cathepsin D levels, the actual levels of total acid protease activity were not significantly higher in renal cancer cells than in normal kidney epithelial cells. 相似文献
854.
Detection of the Leghemoglobin Gene on Two Chromosomes of Phaseolus vulgaris by in situ PCR Linked-Fluorescent in situ Hybridization (FISH) 总被引:1,自引:0,他引:1
Uchiumi Toshiki; Kuwashiro Ryoko; Miyamoto Junko; Abe Mikiko; Higashi Shiro 《Plant & cell physiology》1998,39(7):790-794
The leghemoglobin (Lb) gene on the metaphase chromosomes ofPhaseolus vulgaris was amplified by in situ PCR. The amplifiedLb gene could be detected on two chromosomes by fluorescentin situ hybridization (FISH) using the short Lb gene probe. (Received January 9, 1998; Accepted April 30, 1998) 相似文献