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排序方式: 共有365条查询结果,搜索用时 171 毫秒
91.
Akinori Masuda Takao Yamanaka Tadatoshi Hirakawa Yasuyuki Koga Ryosuke Minomo Takao Munemoto Chuwa Tei 《BioPsychoSocial medicine》2007,1(1):1-7
Many patients with somatoform disorders are frequently encountered in psychosomatic clinics as well as in primary care clinics.
To assess such patients objectively, the concept of somatosensory amplification may be useful. Somatosensory amplification
refers to the tendency to experience a somatic sensation as intense, noxious, and disturbing. It may have a role in a variety
of medical conditions characterized by somatic symptoms that are disproportionate to demonstrable organ pathology. It may
also explain some of the variability in somatic symptomatology found among different patients with the same serious medical
disorder. It has been assessed with a self-report questionnaire, the Somatosensory Amplification Scale. This instrument was
developed in a clinical setting in the U.S., and the reliability and validity of the Japanese and Turkish versions have been
confirmed as well. 相似文献
92.
93.
A Takeno A Okamoto K Tori K Oshima H Hirakawa H Toh N Agata K Yamada N Ogasawara T Hayashi T Shimizu S Kuhara M Hattori M Ohta 《Journal of bacteriology》2012,194(17):4767-4768
We report the complete and annotated genome sequence of Bacillus cereus NC7401, a representative of the strain group that causes emetic-type food poisoning. The emetic toxin, cereulide, is produced by a nonribosomal protein synthesis (NRPS) system that is encoded by a gene cluster on a large resident plasmid, pNCcld. 相似文献
94.
Fukai E Soyano T Umehara Y Nakayama S Hirakawa H Tabata S Sato S Hayashi M 《The Plant journal : for cell and molecular biology》2012,69(4):720-730
We established a gene tagging population of the model legume Lotus japonicus using an endogenous long terminal repeat (LTR) retrotransposon Lotus Retrotransposon 1 (LORE1). The population was composed of 2450 plant lines, from which a total of 4532 flanking sequence tags of LORE1 were recovered by pyrosequencing. The two-dimensional arrangement of the plant population, together with the use of multiple identifier sequences in the primers used to amplify the flanking regions, made it possible to trace insertions back to the original plant lines. The large-scale detection of new LORE1 insertion sites revealed a preference for genic regions, especially in exons of protein-coding genes, which is an interesting feature to consider in the interaction between host genomes and chromoviruses, to which LORE1 belongs, a class of retrotransposon widely distributed among plants. Forward screening of the symbiotic mutants from the population succeeded to identify five symbiotic mutants of known genes. These data suggest that LORE1 is robust as a genetic tool. 相似文献
95.
96.
Kaori Ohtani Hideki Hirakawa Kousuke Tashiro Satoko Yoshizawa Satoru Kuhara Tohru Shimizu 《Anaerobe》2010,16(3):258-264
Clostridium perfringens, a Gram-positive anaerobic pathogen, is a causative agent of human gas gangrene that leads to severe rapid tissue destruction and can cause death within hours unless treated immediately. Production of several toxins is known to be controlled by the two-component VirR/VirS system involving a regulatory RNA (VR-RNA) in C. perfringens. To elucidate the precise regulatory network governed by VirR/VirS and VR-RNA, a series of microarray screening using VirR/VirS and VR-RNA-deficient mutants was performed. Finally, by qRT-PCR analysis, 147 genes (30 single genes and 21 putative operons) were confirmed to be under the control of the VirR/VirS-VR-RNA regulatory cascade. Several virulence-related genes for alpha-toxin, kappa-toxin, hyaluronidases, sialidase, and capsular polysaccharide synthesis were found. Furthermore, some genes for catalytic enzymes, various genes for transporters, and many genes for energy metabolism were also found to be controlled by the cascade. Our data indicate that the VirR/VirS-VR-RNA system is a global gene regulator that might control multiple cellular functions to survive and multiply in the host, which would turn out to be a lethal flesh-eating infection. 相似文献
97.
Yasuko Hirakawa Hiraku Sasaki Eiichi Kawamoto Hiroki Ishikawa Tetsuya Matsumoto Naoki Aoyama Koh Kawasumi Hiromi Amao 《BMC veterinary research》2010,6(1):52
Background
Chinchillas (Chinchilla laniger) are popular as pets and are often used as laboratory animals for various studies. Pseudomonas aeruginosa is a major infectious agent that causes otitis media, pneumonia, septicaemia enteritis, and sudden death in chinchillas. This bacterium is also a leading cause of nosocomial infections in humans. To prevent propagation of P. aeruginosa infection among humans and animals, detailed characteristics of the isolates, including antibiotic susceptibility and genetic features, are needed. In this study, we surveyed P. aeruginosa distribution in chinchillas bred as pets or laboratory animals. We also characterized the isolates from these chinchillas by testing for antibiotic susceptibility and by gene analysis. 相似文献98.
Hiroshi Kawaguchi Keiko Hirakawa Kensuke Miyauchi Kaoru Koike Youkichi Ohno Atsuhiro Sakamoto 《PloS one》2010,5(6)
Background
General anesthesia is routinely used as a surgical procedure and its safety has been endorsed by clinical outcomes; however, its effects at the molecular level have not been elucidated. General anesthetics influence glucose metabolism in the brain. However, the effects of anesthetics on brain metabolites other than those related to glucose have not been well characterized. We used a pattern recognition analysis of proton nuclear magnetic resonance spectra to visualize the changes in holistic brain metabolic phenotypes in response to the widely used intravenous anesthetic propofol and the volatile anesthetic isoflurane.Methodology/Principal Findings
Rats were randomized into five groups (n = 7 each group). Propofol and isoflurane were administered to two groups each, for 2 or 6 h. The control group received no anesthesia. Brains were removed directly after anesthesia. Hydrophilic compounds were extracted from excised whole brains and measured by proton nuclear magnetic resonance spectroscopy. All spectral data were processed and analyzed by principal component analysis for comparison of the metabolite profiles. Data were visualized by plotting principal component (PC) scores. In the plots, each point represents an individual sample. The propofol and isoflurane groups were clustered separately on the plots, and this separation was especially pronounced when comparing the 6-h groups. The PC scores of the propofol group were clearly distinct from those of the control group, particularly in the 6-h group, whereas the difference in PC scores was more subtle in the isoflurane group and control groups.Conclusions/Significance
The results of the present study showed that propofol and isoflurane exerted differential effects on holistic brain metabolism under anesthesia. 相似文献99.
Yoshinao Azuma Hideki Hirakawa Atsushi Yamashita Yan Cai Mohd Akhlakur Rahman Harumi Suzuki Shigeki Mitaku Hidehiro Toh Susumu Goto Tomoyuki Murakami Kazuro Sugi Hideo Hayashi Hideto Fukushi Masahira Hattori Satoru Kuhara Mutsunori Shirai 《DNA research》2006,13(1):15-23
Chlamydophila felis (Chlamydia psittaci feline pneumonitis agent) is a worldwide spread pathogen for pneumonia and conjunctivitis in cats. Herein, we determined the entire genomic DNA sequence of the Japanese C. felis strain Fe/C-56 to understand the mechanism of diseases caused by this pathogen. The C. felis genome is composed of a circular 1,166,239 bp chromosome encoding 1005 protein-coding genes and a 7552 bp circular plasmid. Comparison of C. felis gene contents with other Chlamydia species shows that 795 genes are common in the family Chlamydiaceae species and 47 genes are specific to C. felis. Phylogenetic analysis of the common genes reveals that most of the orthologue sets exhibit a similar divergent pattern but 14 C. felis genes accumulate more mutations, implicating that these genes may be involved in the evolutional adaptation to the C. felis-specific niche. Gene distribution and orthologue analyses reveal that two distinctive regions, i.e. the plasticity zone and frequently gene-translocated regions (FGRs), may play important but different roles for chlamydial genome evolution. The genomic DNA sequence of C. felis provides information for comprehension of diseases and elucidation of the chlamydial evolution. 相似文献
100.
Nagao K Sakono M Nakayama M Hirakawa T Imaizumi K 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1999,124(3):289-294
The role of gemfibrozil, a hypotriglyceridemic drug, in synthesis, secretion and catabolism of triacylglycerols (TG) in rats was assessed. Chow diet-fed Sprague-Dawley rats were given various doses of gemfibrozil (10, 30 and 100 mg/kg body weight) for 2 weeks. Rats receiving the drug at the lowest dose significantly lowered the concentration of serum TG and apolipoprotein (apo) B in comparison with control rats. Synthesis of fatty acids from [14C]acetate and esterification of [14C]oleate to TG by the liver were not suppressed by the drug. Secretion rates of TG and apo B, measured by the Triton method, were suppressed at the highest dose. Lipoprotein lipase activity of the acetone powder prepared from adipose tissue was not influenced by the drug. These results indicate that the primary cause of hypotriglyceridemic action of gemfibrozil is not due to suppressing synthesis and secretion of TG by the liver or enhancing lipoprotein lipase activity in adipose tissues. 相似文献