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61.
Close linkage of MEN2A with RBP3 locus in Japanese kindreds 总被引:7,自引:0,他引:7
Masayuki Yamamoto Shin-ichiro Takai Tetsuro Miki Kazuyoshi Motomura Makoto Okazaki Isamu Nishisho Hideo Tateishi Akira Miyauchi Tasuku Honjo A. J. Pakstis Takesada Mori 《Human genetics》1989,81(3):287-288
Summary The gene responsible for multiple endocrine neoplasia type 2A (MEN2A) has recently been assigned to the pericentromeric region of chromsome 10 in European Caucasian kindreds by linkage analysis using a DNA marker, interstitial retinol-binding protein 3 (RBP3). We have found tight linkage between the MEN2A and RBP3 loci in Japanese MEN2A kindreds. The maximum lod score is 5.19 at a recombination fraction of 0.00. This result suggests that mutation of a certain gene close to RBP3 is responsible for MEN2A irrespective of ethnic backgrounds. 相似文献
62.
Tadao Niijima Takashi Umeda Manabu Kuriyama Hiroyuki Ohmori Yohsuke Matsumura Tomoyasu Tsushima Toyoko Tanahashi Jun Yoshimoto Toshihiko Asahi Norimasa Ike Taiichiro Johsen Noritaka Ishido Naoki Mitsuhata Takeshi Uyama Hiroyoshi Tanaka Hideo Ueda Jisaburo Sakatoku Norio Yamamoto Kazuo Nagata Yukitoshi Fujita Masaaki Morioka Kazuo Kurokawa Susumu Kagawa Tomoyuki Ishibe Yasutoshi Himeno Toyofumi Ueda 《Cancer immunology, immunotherapy : CII》1989,30(2):81-85
Summary In order to examine its clinical efficacy, recombinant human interferon- (rIFN-) was instilled intravesically into 51 patients with superficial bladder cancer. Ten patients, who received intermittent intravesical instillation at a dose of (3–36) × 106 U rIFN- on days 1–3 every week, showed no response. Thirty-two patients received intravesical instillation at a dose of (3–36) × 106 U every day for 10–20 days. Eight patients showed partial response, indicating an efficacy rate of 25%. Nine patients received divided doses of 18 × 106 U twice a day every day for 10–20 days. Six patients showed partial response, indicating an efficacy rate of 67%. This value was significantly higher than that obtained by administering divided doses. The response to intravesical instillation therapy with rIFN- varies with treatment protocol. Frequent and longer exposure to rIFN- may induce better regression of superficial bladder cancer. Six incidences of side-effects were found in five cases (9.8%): pollakiuria in one, pain on micturition in two, fever in two, and eruption in one case. All of these side-effects were slight and reversible after drug withdrawal. Laboratory tests showed only a few changes with low severity. Thus, rIFN- is potentially a new drug for instillation therapy of superficial bladder cancer, in view of the absence of adverse effects. 相似文献
63.
Copulatory behavior unrelated to conception is sometimes observed in some non-human primates including the Japanese monkey.
In the present study, the authors examined whether a mature follicle or a newly formed fresh corpus luteum was observed in
the ovaries of female Japanese monkeys which displayed the copulatory behavior unrelated to conception.
Post-conception copulatory behaviors were observed in three out of four females usually kept in individual cages in an air-conditioned
room, and in two out of three females without infants kept in an outdoor group cage. However, neither a mature Graafian follicle
nor a fresh corpus luteum formed newly after conception was observed in any of these females by laparoscopic examinations
conducted immediately after termination of the copulatory behavior. In females with infants born in the preceding birth season,
copulatory behaviors were observed in three out of four females kept in the outdoor group cage, and in two out of four females
in a free-ranging troop. Ovulation was confirmed in one case out of the three kept in the outdoor group cage, but neither
a mature follicle nor a newly formed corpus luteum was observed in the remaining four females.
These findings suggest that copulatory behavior in the Japanese monkey is not always controlled by the development of a follicle
or ovulation in the ovary. 相似文献
64.
Y Chatani A Itoh E Tanaka A Hattori T Nakamura M Kohno 《Biochemical and biophysical research communications》1992,185(3):860-866
We have examined the hepatocyte growth factor (HGF)-mediated changes in protein-tyrosine phosphorylation in mouse keratinocytes (PAM-212) and canine kidney epithelial cells (MDCK). In PAM-212 cells HGF and epidermal growth factor, both of which stimulated the DNA synthesis, rapidly induced the tyrosine phosphorylation of two 41-kDa and two 43-kDa proteins: increased tyrosine phosphorylation of those proteins has been commonly observed when quiescent fibroblasts are stimulated with a variety of mitogenic agents. In contrast, HGF did not stimulate the DNA synthesis but induced cell dissociation in MDCK cells; under this condition, increased tyrosine phosphorylation of the 41-kDa and 43-kDa protein was not observed. A possible role of the increased tyrosine phosphorylation of 41-kDa and 43-kDa protein in the signaling pathway of HGF is discussed. 相似文献
65.
Reversal of relative proteinase F activity and onset of androgen-dependent proteinases in the submandibular gland of postnatal mice 总被引:1,自引:0,他引:1
By isoelectric focusing, we separated trypsin-like proteinases of the mouse submandibular gland (ICR strain) into isozymes with pI values of 4.6 (proteinase F), 5.6 (protease D), 5.8 (protease A), 7.1 and 9.9 (P-esterase). During postnatal development, proteinase F appeared earliest (on the 15th day after birth) and increased in both sexes; however, its percentage ratio to total activity decreased markedly with time because of the rapid increase of other proteinases. On the 22nd day of life, proteinases A and D appeared, and the increase of a proteinase with pI-7.1 followed thereafter. P-esterase was the last isozyme to appear, becoming detectable around 29-45 days. After maturation, the activities of protease A plus D, P-esterase, and the isozyme with a pI value of 7.1 were higher in males than in females, whereas the relative level of proteinase F was reversed. We conclude that proteinase F is appreciably different from the other four proteinases in its development pattern as well as in its responsiveness to sexual hormones. 相似文献
66.
67.
Akira Yamada Masaaki Murakami Kenichi Ijima Hideo Yagita Ko Okumura Sakuzo Komatsu Toshimitsu Uede 《Microbiology and immunology》1996,40(7):513-518
The immunosuppressant FK506 prolongs allograft survival. However, at therapeutic doses it has significant side effects. A fusion protein consisting of the extracellular portion of CTLA4 and the Fc portion of human IgG (CTLA4IgG) also prolongs allograft survival, but large doses of CTLA4IgG are required for the induction of cardiac allograft acceptance. Therefore, we constructed a pentameric form of a new CTLA4 fusion protein, CTLA4IgM. We tested whether low doses of CTLA4IgG or CTLA4IgM in combination with subtherapeutic doses of FK506 can prolong allograft survival in a synergistic fashion. C57BL/6 (H-2b) neonatal hearts were transplanted to CBA/J (H-2k) mice in a heterotopic, nonvascularized cardiac allograft model. The findings demonstrate that a combination of low doses of FK506 plus a pentameric form of CTLA4Ig, CTLA4IgM, leads to significant graft survival, while a combination of FK506 plus CTLA4IgG does not. 相似文献
68.
We examined the applicability of radiotelemetry to studies of acorn dispersal byApodemus mice and compared its efficiency with the of this spool-and-line method. Installation of a transmitter (2.2 g) onto acorns
did not interfere with the transporting and feeding behavior of the mice. We were able to detect all transmitter-installed
acorns and follow the daily changes in the sites in which they were hoarded, while we missed 59% of the spool-tied acorns
due to mice breaking the threads. Mice carried transmitter-installed acorns farther than spool-tied ones. The radiotelemetry
method is superior to the spool-and-line method and useful for the study of hoarding behavior in rodents. 相似文献
69.
Chieko Takaya Ayako Kosaka Kyouko Kohno Toshihisa Kusano Ken-Ichi Nakamura 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1995,112(4):727-732
Tetrahymena thermophila could still swim after incubation of the cell body at 40°C for 30 min, whereas Tetrahymena pyriformis did not show any motility after the treatment. Turbidity measurements revealed that axonemes of T. pyriformis lost ATP-dependent sliding activity by the heat treatment, whereas those of T. thermophilia still had the activity under the same conditions. In connection with this difference in susceptibility to high temperature, the biochemical characteristics of dyneins were compared between the two species of Tetrahymena. Axonemal dyneins from the two species had significant vanadate-sensitive ATPase activity even after the heat treatment. Native gel electrophoresis and the following two-dimensional electrophoresis showed that the outer arm dynein of T. thermophilia is more stable in maintaining native configuration than that of T. pyriformis against the heat treatment, although both treated dyneins keep three (α, β and γ) subunits. Analysis by peptide mapping demonstrated that β- and γ-subunits of the outer arm dynein are considerably different in amino acid sequences between the two species. These results imply that dynein of T. thermophilia changed their amino acid sequences and biochemical characteristics to adapt to high temperature. 相似文献
70.
Ken-ichi Tanaka Shigeru Kohno Takashige Miyazaki Haruko Miyazaki Kotaro Mitsutake Shigefumi Maesaki Mitsuo Kaku Hironobu Koga 《Mycopathologia》1994,127(3):131-134
A latex agglutination test for cryptococcal antigen, the Eiken Latex test (Eiken, Tokyo, Japan), was compared with a monoclonal antibody-based agglutination assay, Pastorex® Cryptococcus (Diagnostics Pasteur, Marneur-la-Coquette, France). In a murine model of disseminated cryptococcosis, the kinetics of the antigen titers by the Eiken Latex were similar to those by the Pastorex® Cryptococcus, but sensitivity was much higher. In HIV-negative patients with pulmonary cryptococcosis, a cryptococcal antigen was detected in 6 of 10 patients by the Eiken Latex test and in only 3 of those patients by the Pastorex® Cryptococcus. The results indicate that the Eiken Latex is more sensitive for the detection of the cryptococcal antigen, even in non-disseminated cryptococcosis. The sensitivity and specificity of the Eiken Latex were examined using 195 sera from 25 patients with pulmonary cryptococcosis and 170 patients with non-cryptococcosis. The cutoff value of 1:8 showed a sensitivity of 76% (19/25) and a specificity of 98.9% (168/170). 相似文献