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51.
Yakabe S Soejima H Yatsuki H Tominaga H Zhao W Higashimoto K Joh K Kudo S Miyazaki K Mukai T 《Genes & genetic systems》2008,83(2):199-208
52.
Kobayashi N Kuniyoshi H Ishigami K Watanabe H 《Bioscience, biotechnology, and biochemistry》2008,72(10):2708-2715
Both enantiomers of FF8181-A were synthesized through optical resolution from the known Diels-Alder reaction product in 15 steps. The absolute configuration of the natural product was determined to be 1S,5S,5aS,9aS,9bS. 相似文献
53.
Tashiro Y Nomura N Nakao R Senpuku H Kariyama R Kumon H Kosono S Watanabe H Nakajima T Uchiyama H 《Journal of bacteriology》2008,190(11):3969-3978
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that is one of the most refractory to therapy when it forms biofilms in the airways of cystic fibrosis patients. To date, studies regarding the production of an immunogenic and protective antigen to inhibit biofilm formation by P. aeruginosa have been superficial. The previously uncharacterized outer membrane protein (OMP) Opr86 (PA3648) of P. aeruginosa is a member of the Omp85 family, of which homologs have been found in all gram-negative bacteria. Here we verify the availability of Opr86 as a protective antigen to inhibit biofilm formation by P. aeruginosa PAO1 and several other isolates. A mutant was constructed in which Opr86 expression could be switched on or off through a tac promoter-controlled opr86 gene. The result, consistent with previous Omp85 studies, showed that Opr86 is essential for viability and plays a role in OMP assembly. Depletion of Opr86 resulted in streptococci-like morphological changes and liberation of excess membrane vesicles. A polyclonal antibody against Opr86 which showed reactivity to PAO1 cells was obtained. The antibody inhibited biofilm formation by PAO1 and the other clinical strains tested. Closer examination of early attachment revealed that cells treated with the antibody were unable to attach to the surface. Our data suggest that Opr86 is a critical OMP and a potential candidate as a protective antigen against biofilm formation by P. aeruginosa. 相似文献
54.
Tetsuro Tamaki Yoshiyasu Uchiyama Yoshinori Okada Kayoko Tono Masahiro Nitta Akio Hoshi Akira Akatsuka 《Histochemistry and cell biology》2009,132(1):59-70
Tissue inflammation and multiple cellular responses in the compensatory enlarged plantaris (OP Plt) muscle induced by surgical
ablation of synergistic muscles (soleus and gastrocnemius) were followed over 10 weeks after surgery. Contralateral surgery
was performed in adult Wistar male rats. Cellular responses in muscle fibers, blood vessels and nerve fibers were analyzed
by immunohistochemistry and electron microscopy. Severe muscle fiber damage and disappearance of capillaries associated with
apparent tissue edema were observed in the peripheral portion of OP Plt muscles during the first week, whereas central portions
were relatively preserved. Marked cell activation/proliferation was also mainly observed in peripheral portions. Similarly,
activated myogenic cells were seen not only inside but also outside of muscle fibers. The former were likely satellite cells
and the latter may be interstitial myogenic cells. One week after surgery, small muscle fibers, small arteries and capillaries
and several branched-muscle fibers were evident in the periphery, thus indicating new muscle fiber and blood vessel formation.
Proliferating cells were also detected in the nerve bundles in the Schwann cell position. These results indicate that the
compensatory stimulated/enlarged muscle is a suitable model for analyzing multiple physiological cellular responses in muscle–nerve–blood
vessel units under continuous stretch stimulation.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
55.
56.
Nascent polypeptide chains synthesized by membrane bound ribosomes are cotranslationally translocated through and integrated into the endoplasmic reticulum translocon. Hydrophobic segments and positive charges on the chain are critical to halt the ongoing translocation. A marginally hydrophobic segment, which cannot be inserted into the membrane by itself, can be a transmembrane segment depending on its downstream positive charges. In certain conditions, positive charges even 60 residues downstream cause the marginally hydrophobic segment to span the membrane by inducing the segment to slide back from the lumen. Here we systematically examined the effect of a core sugar chain on the fate of a marginally hydrophobic segment using a cell-free translation and translocation system. A sugar chain added within 12 residues upstream of the marginally hydrophobic segment prevents the sliding back and promotes forward movement of the polypeptide chain. The sugar chain apparently functions as a ratchet to keep the polypeptide chain in the lumen. We propose that the sugar chain is a third topology determinant of membrane proteins, in addition to a hydrophobic segment and positive charges of the nascent chain. 相似文献
57.
58.
Hiroaki Asai Hiroshi Fujiwara Sohei Kitazawa Naoto Kobayashi Toshiki Ochi Yukihiro Miyazaki Fumihiro Ochi Yoshiki Akatsuka Sachiko Okamoto Junichi Mineno Kiyotaka Kuzushima Hiroaki Ikeda Hiroshi Shiku Masaki Yasukawa 《Journal of hematology & oncology》2014,7(1):1-4
Because WT1 is expressed in leukemia cells, the development of cancer immunotherapy targeting WT1 has been an attractive translational research topic. However, concern of this therapy still remains, since WT1 is abundantly expressed in renal glomerular podocytes. In the present study, we clearly showed that WT1-specific cytotoxic T lymphocytes (CTLs) certainly exerted cytotoxicity against podocytes in vitro; however, they did not damage podocytes in vivo. This might be due to the anatomical localization of podocytes, being structurally separated from circulating CTLs in glomerular capillaries by an exceptionally thick basement membrane. 相似文献
59.
Thymic stromal cell clone with nursing activity supports the growth and differentiation of murine CD4+8+ thymocytes in vitro 总被引:2,自引:0,他引:2
T Nishimura Y Takeuchi Y Ichimura X H Gao A Akatsuka N Tamaoki H Yagita K Okumura S Habu 《Journal of immunology (Baltimore, Md. : 1950)》1990,145(12):4012-4017
Thymic stromal cell clone, TNC-R3.1 cell, was established from spontaneous AKR/J mouse thymoma. TNC-R3.1 cell, which has the similar properties to thymic nurse cells, formed a unique complex with normal thymocyte subpopulations. Flow cytometry analysis demonstrated that CD4+8+ and CD4-8- immature thymocytes preferentially interacted with TNC-R3.1 stromal cell clone. CD4+8+ thymocytes, which interacted with TNC-R3.1 stromal cell clone, contained a higher proportion of large size and cycling T cells than did noninteracting CD4+8+ thymocytes. As is generally accepted, CD4+8+ thymocytes did not respond to any stimulation such as IL-2, anti-CD3 mAb (2C11), or IL-2 plus 2C11. However, culture of isolated CD4+8+ thymocytes on TNC-R3.1 stromal cell monolayer in the presence of suboptimal dose of IL-2 induced a significant cell growth. Moreover, the addition of 2C11 and IL-2 into this coculture system resulted in a dramatic increase of the proliferative response of thymocytes. Flow cytometry analysis showed the proliferating cells on TNC-R3.1, which originated from CD4+8+ thymocytes, were mostly TCR-alpha beta+ CD3+CD4-8+ T cells. These results provide in vitro evidence that CD4+8+ thymocytes are at an intermediate stage of T cell maturation and TNC-R3.1 stromal cell clone induces the growth and differentiation of CD4+8+ thymocytes into CD4-8+ T cells. 相似文献
60.