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81.
Yoshiaki Fujimiya Youichi Suzuki Ko-ichi Oshiman Hidekazu Kobori Koichi Moriguchi Hisako Nakashima Yonezo Matumoto Shogo Takahara Takusaburo Ebina Ryuichi Katakura 《Cancer immunology, immunotherapy : CII》1998,46(3):147-159
We have isolated a novel type of natural tumoricidal product from the basidiomycete strain, Agaricus blazei Murill. Using the double-grafted tumor system in Balb/c mice, treatment of the primary tumor with an acid-treated fraction
(ATF) obtained from the fruit bodies resulted in infiltration of the distant tumor by natural killer (NK) cells with marked
tumoricidal activity. As shown by electrophoresis and DNA fragmentation assay, the ATF also directly inhibited tumor cell
growth in vitro by inducing apoptotic processing; this apoptotic effect was also demonstrated by increased expression of the
Apo2.7 antigen on the mitochondrial membranes of tumor cells, as shown by flow-cytometric analysis. The ATF had no effect
on normal mouse splenic or interleukin-2-treated splenic mononuclear cells, indicating that it is selectively cytotoxic for
the tumor cells. Cell-cycle analysis demonstrated that ATF induced the loss of S phase in MethA tumor cells, but did not affect
normal splenic mononuclear cells, which were mainly in the G0G1 phase. Various chromatofocussing purification steps and NMR
analysis showed the tumoricidal activity to be chiefly present in fractions containing (1→4)-α-D-glucan and (1→6)-β-D-glucan, present in a ratio of approximately 1:2 in the ATF (molecular mass 170 kDa), while the final purified fraction, HM3-G
(molecular mass 380 kDa), with the highest tumoricidal activity, consisted of more than 90% glucose, the main component being
(1→4)-α-D-glucan with (1→6)-β branching, in the ratio of approximately 4:1.
Received: 27 August 1997 / Accepted 22 December 1997 相似文献
82.
Efficient isolation of human parainfluenza viruses 1 and 3 using MNT‐1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect
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Ko Sato Oshi Watanabe Suguru Ohmiya Fumiko Chiba Masahiro Hayashi Tamio Suzuki Kazuyoshi Kawakami Hidekazu Nishimura 《Microbiology and immunology》2016,60(11):801-805
Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT‐1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT‐1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT‐1 cell culture system has the potential to improve virus isolation from clinical specimens. 相似文献
83.
Epidermal growth factor signals attenuate phenotypic and functional development of neocortical GABA neurons
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84.
Joji Ohshita Hidekazu Arase Nobuhisa Mimura Yosuke Tada Yutaka Harima 《Inorganica chimica acta》2005,358(14):4156-4162
UV irradiation of poly(organosilanylene-2,5-diethynylenesiloles) in benzene with an excess of Fe(CO)5 led to the formation of Fe(CO)3-coordinated silole units in the polymer backbone. The Fe(CO)3-coordinated polymers exhibited suppressed π-conjugation, relative to the parent non-coordinated polymers. Hole-transporting properties of poly(organosilanylene-2,5-diethynylenesiloles) were examined by the performance of EL devices containing the polymer layer as the hole-transport and Alq3 layer as the electron-transporting emitter. 相似文献
85.
Antonysamy SS Aubol B Blaney J Browner MF Giannetti AM Harris SF Hébert N Hendle J Hopkins S Jefferson E Kissinger C Leveque V Marciano D McGee E Nájera I Nolan B Tomimoto M Torres E Wright T 《Bioorganic & medicinal chemistry letters》2008,18(9):2990-2995
Non-nucleoside inhibitors of HCV NS5b RNA polymerase were discovered by a fragment-based lead discovery approach, beginning with crystallographic fragment screening. The NS5b binding affinity and biochemical activity of fragment hits and inhibitors was determined by surface plasmon resonance (Biacore) and an enzyme inhibition assay, respectively. Crystallographic fragment screening hits with 1–10 mM binding affinity (KD) were iteratively optimized to give leads with 200 nM biochemical activity and low μM cellular activity in a Replicon assay. 相似文献
86.
Small hepatocytes (SHs) are hepatic progenitor cells with hepatic characteristics. They can proliferate to form colonies in culture and change their morphology from flat to rising/piled-up with bile canaliculi (BC), which results in maturation. In this study, we examined whether SHs could express hepatic transporters with polarity, whether the transporters could transport organic anion substrates into BC, and whether the secreted substances could be recovered from BC. Immunocytochemistry and RT-PCR were carried out. [(3)H]-labeled estrogen derivatives were used to measure the functions of the transporters in SHs isolated from normal and multidrug resistance-associated protein (Mrp) 2-deficient rats. The results showed that organic anion-transporting proteins (Oatps) 1 and 2, Na(+)-dependent taurocholate co-transporting polypeptide (Ntcp), Mrp2, and bile-salt export pump (Bsep) were well expressed in rising/piled-up cells and that their expression was correlated to that of hepatocyte nuclear factor 4alpha. Although small SHs expressed not Oatps and Mrp2 but Mrp3, rising/piled-up SHs expressed Oatp1 and 2 and Mrp2 proteins in the sinusoidal and BC membranes, respectively. On the other hand, breast cancer resistant protein (Bcrp) and Mrp3 expression decreased as SHs matured. The substrate transported via Oatps and Mrp2 was secreted into BC and it accumulated in both BC and cyst-like structures. The secreted substrate could be efficiently recovered from BC reconstructed by SHs derived from a normal rat, but not from an Mrp2-deficient rat. In conclusion, SHs can reconstitute hepatic organoids expressing functional organic anion transporters in culture. This culture system may be useful to analyze the metabolism and excretion mechanisms of drugs. 相似文献
87.
Kuwano Y Tominaga K Kawahara T Sasaki H Takeo K Nishida K Masuda K Kawai T Teshima-Kondo S Rokutan K 《Free radical biology & medicine》2008,45(12):1642-1652
88.
Yukihiko Hayashi Moto Kitayaki Kayoko Takezaki Hidekazu Ikeuchi Shigeru Kunugi 《Biocatalysis and Biotransformation》2003,21(1):25-31
The contribution of three exoglucanases from a commercial Trichoderma viride cellulase to transcellobiosylation, and the tolerance of these enzymes to acetonitrile co-solvent were studied. The enzymatic reactions were performed with p-nitrophenyl-β-d-cellobioside as the starting substrate. Among these enzymes, the least anionic exoglucanase (Exo I) showed the highest transcellobiosylation activity and acetonitrile tolerance. Exo I retained considerable activity even in 30% MeCN/water and produced p-nitrophenyl-β-d-cellotetraoside at about 1.5% conversion from the initial substrate in 30% MeCN/water. The residual activity of Exo I after incubation in MeCN/water mixture was almost identical to that of the crude cellulase and a considerable amount of the transcellobiosylation properties of the crude cellulase seemed to be attributable to this Exo I component. 相似文献
89.
90.
Makiko Suto Hidekazu Tanaka Yasuhide Mochizuki Jun Mukai Hiroki Takada Fumitaka Soga Kumiko Dokuni Yutaka Hatani Keiko Hatazawa Hiroki Matsuzoe Hiroyuki Sano Hiroyuki Shimoura Junichi Ooka Kensuke Matsumoto Yushi Hirota Wataru Ogawa Ken-ichi Hirata 《Cardiovascular diabetology》2017,16(1):145