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排序方式: 共有158条查询结果,搜索用时 31 毫秒
51.
52.
Urata H Nomura K Wada S Akagi M 《Biochemical and biophysical research communications》2007,360(2):459-463
One of the intriguing applications of aptamers is sensing molecules. In principle, an aptamer can specifically recognize and bind to a unique ligand, leading to a structural change of an aptamer. By acquiring information for the structural change, the detection of the ligand can be achieved. To design and explore an aptamer molecule to detect adenosine, we have synthesized some ATP aptamer variants labeled with donor and acceptor fluorophores. Although the fluorescent response of the aptamer variants was highly dependent on experimental temperature, we have found one of the variants showing suitable fluorescent response by titration with adenosine. The aptamer variant showed remarkable selectivity for adenosine over the other ribonucleosides. On the other hand, the enantio-specificity of the aptamer variant in the ligand recognition was not enough to selectively detect d-adenosine over l-adenosine. 相似文献
53.
Dalvin S Anselmo MA Prodhan P Komatsuzaki K Schnitzer JJ Kinane TB 《Gene expression patterns : GEP》2003,3(3):279-283
The ligand Netrin-1 and its receptors DCC and UNC5H2 are critical for the regulation of neuronal migration in nervous system development. Here we demonstrate expression of these molecules in lung development. The mRNA expression profiles of Netrin-1, DCC and UNC5H2 are developmentally regulated during embryonic mouse lung formation. Netrin-1 shows a bimodal expression pattern with elevated mRNA levels early followed by a second peak in late gestation. Peak expression of DCC occurs early in development whereas expression of UNC5H2 peaks late in development. We also demonstrate localization of Netrin-1, DCC and UNC5H2 during the stages of lung development. We present evidence that these proteins are modulated spatially in the mesenchyme and epithelium during lung organogenesis. 相似文献
54.
Eichi Watabe Marina TogoOhno Yuma Ishigami Shotaro Wani Keiko Hirota Mariko KimuraAsami Sharmin Hasan Satomi Takei Akiyoshi Fukamizu Yutaka Suzuki Tsutomu Suzuki Hidehito Kuroyanagi 《The EMBO journal》2021,40(14)
Alternative splicing of pre‐mRNAs can regulate gene expression levels by coupling with nonsense‐mediated mRNA decay (NMD). In order to elucidate a repertoire of mRNAs regulated by alternative splicing coupled with NMD (AS‐NMD) in an organism, we performed long‐read RNA sequencing of poly(A)+ RNAs from an NMD‐deficient mutant strain of Caenorhabditis elegans, and obtained full‐length sequences for mRNA isoforms from 259 high‐confidence AS‐NMD genes. Among them are the S‐adenosyl‐L‐methionine (SAM) synthetase (sams) genes sams‐3 and sams‐4. SAM synthetase activity autoregulates sams gene expression through AS‐NMD in a negative feedback loop. We furthermore find that METT‐10, the orthologue of human U6 snRNA methyltransferase METTL16, is required for the splicing regulation in␣vivo, and specifically methylates the invariant AG dinucleotide at the distal 3′ splice site (3′SS) in␣vitro. Direct RNA sequencing coupled with machine learning confirms m6A modification of endogenous sams mRNAs. Overall, these results indicate that homeostasis of SAM synthetase in C. elegans is maintained by alternative splicing regulation through m6A modification at the 3′SS of the sams genes. 相似文献
55.
Shun-ichi Wada Anna Takesada Yurie Nagamura Eri Sogabe Rieko Ohki Junsuke Hayashi Hidehito Urata 《Bioorganic & medicinal chemistry letters》2017,27(24):5378-5381
The conjugation of Aib-containing amphipathic helical peptide with cyclo(-Arg-Gly-Asp-d-Phe-Cys-) (cRGDfC) at the C-terminus of the helix peptide (PI) has been reported to be useful for constructing a carrier for targeted siRNA delivery into cells. In order to explore structure–activity relationships for the development of potential carriers for siRNA delivery, we synthesized conjugates of Aib-containing amphipathic helical peptide with cRGDfC at the N-terminus (PII) and both the N- and C-termini (PIII) of the helical peptide. Furthermore, to examine the influence of PI helical chain length on siRNA delivery, truncated peptides containing 16 (PIV), 12 (PV), and 8 (PVI) amino acid residues at the N-terminus of the helical chain were synthesized. PII and PIII, as well as PI, could deliver anti-luciferase siRNA into cells to induce the knockdown of luciferase stably expressed in cells. In contrast, all of the truncated peptides were unlikely to transport siRNA into cells. 相似文献
56.
K Komatsuzaki K Terashita T B Kinane I Nishimoto 《Biochemical and biophysical research communications》2001,289(5):1211-1217
Somatostatin is a neurotransmitter with diverse effects including anti-proliferation in a wide range of normal and neoplastic cells, and occasionally growth stimulatory and neurotrophic actions. Stress-activated protein kinase or c-Jun N-terminal kinase (SAPK/JNK) can also induce growth arrest and occasionally growth stimulation. However, the relationship between somatostatin and SAPK/JNK is less clear. Here we report that the binding of somatostatin to the somatostatin receptor type V (SSTR5) upregulates SAPK/JNK activity. We also show that this activation is mediated by Galpha(12) and Galpha(13). This study demonstrates that SSTR5 is the heptahelical receptor that activates SAPK/JNK via the G(12) family G proteins. 相似文献
57.
Hidehito Urata Masayuki Urata Masao Akagi Hidekazu Hiroaki Seiich Uesugi 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1259-1269
Abstract We investigated the structure of a decadeoxynucleotide duplex which was modified with Dichloroethylenediamineplatinum(II) (PtCl2(en)) at the central GG site by using some spectroscopic techniques. The results suggest that the DNA structural changes induced by binding of PtCl2(en) are quite similar to those of cisplatin. 相似文献
58.
59.
Hidehito Kotani Markus W. Germann Alex Andrus Ravi Vinayak Bashar Mullah Eric B. Kmiec 《Molecular & general genetics : MGG》1996,250(5):626-634
The RecA protein ofEscherichia coli catalyzes homologous pairing and strand exchange between a wide range of molecules showing nucleotide sequence complementarity, including a linear duplex and a single-stranded DNA molecule. We demonstrate that RecA can promote formation of joint molecules when the duplex contains an RNA/DNA hairpin and a single-stranded circle serves as the pairing partner. A chimeric RNA/DNA hairpin can be used to form stable joint molecules with as little as 15 bases of shared homology as long as the RNA stretch contains complementarity to the circle. The joint molecule bears some resemblance to a triple helical structure composed of RNA residues surrounded by two DNA strands which are in a parallel orientation. Evidence is presented that supports the notion that short stretches of RNA can be used in homologous pairing reactions at lengths below that required for DNA-DNA heteroduplex formation. 相似文献
60.
Mitsuhara Ichiro; Ugaki Masashi; Hirochika Hirohiko; Ohshima Masahiko; Murakami Taka; Gotoh Yoko; Katayose Yuichi; Nakamura Shigeo; Honkura Ryoso; Nishimiya Satoshi; Ueno Keiichiro; Mochizuki Atsushi; Tanimoto Hideo; Tsugawa Hidehito; Otsuki Yoshiaki; Ohashi Yuko 《Plant & cell physiology》1996,37(1):49-59