全文获取类型
收费全文 | 3055篇 |
免费 | 163篇 |
国内免费 | 1篇 |
专业分类
3219篇 |
出版年
2022年 | 14篇 |
2021年 | 29篇 |
2020年 | 12篇 |
2019年 | 20篇 |
2018年 | 31篇 |
2017年 | 39篇 |
2016年 | 50篇 |
2015年 | 83篇 |
2014年 | 101篇 |
2013年 | 297篇 |
2012年 | 160篇 |
2011年 | 143篇 |
2010年 | 122篇 |
2009年 | 110篇 |
2008年 | 179篇 |
2007年 | 189篇 |
2006年 | 214篇 |
2005年 | 197篇 |
2004年 | 210篇 |
2003年 | 178篇 |
2002年 | 152篇 |
2001年 | 35篇 |
2000年 | 31篇 |
1999年 | 51篇 |
1998年 | 29篇 |
1997年 | 36篇 |
1996年 | 31篇 |
1995年 | 32篇 |
1994年 | 35篇 |
1993年 | 28篇 |
1992年 | 44篇 |
1991年 | 35篇 |
1990年 | 42篇 |
1989年 | 22篇 |
1988年 | 34篇 |
1987年 | 21篇 |
1986年 | 15篇 |
1985年 | 20篇 |
1984年 | 16篇 |
1983年 | 9篇 |
1982年 | 17篇 |
1981年 | 14篇 |
1980年 | 9篇 |
1979年 | 12篇 |
1978年 | 12篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1975年 | 7篇 |
1974年 | 6篇 |
1973年 | 6篇 |
排序方式: 共有3219条查询结果,搜索用时 0 毫秒
111.
Fusako Kawai Kensei Maezato Hideaki Yamada Koichi Ogata 《Bioscience, biotechnology, and biochemistry》2013,77(9):1675-1679
The formation of D-pantothenic acid-α-glucoside (PaA-α-G) was found from D-pantothenic acid (PaA) and maltose in incubation mixtures of microorganisms, especially Saccharomyces yeasts and Sporobolomyces coralliformis IFO 1032. The reaction conditions were investigated for formation of PaA-α-G by resting cells of Spor. coralliformis. The formation of the compound increased with PaA concentration (3~20 mg/ml). The yield was maximum at 5~10 mg/ml of PaA. Cetyl trimethyl ammonium bromide (0.1 %) promoted the formation of PaA-α-G. Sucrose was the optimal α-glucosyl donor. When 30 mg/ml of sucrose was fed to the reaction mixture (initial sucrose, 100 mg/ml; and PaA, 10 mg/ml) at 12-hr intervals, 5.74 mg/ml (3.30 mg/ml as PaA) of PaA-α-G was formed in 48-hr incubation at 28°C with shaking. PaA-α-G was also formed by yeast α-glucosidase, mold maltase and the cell-free extract of Spor. coralliformis. The compound showed approximately 9~10% and 0.1~0.3% (molar ratio) of activity of PaA for Saccharomyces carlsbergensis ATCC 9080 and Lactobacillus plantarum ATCC 8014, respectively. The compound had the same microbiological activity as authentic 4′-O-(α-D-glucopyranosyl)-D-pantothenic acid. 相似文献
112.
113.
Hajime Yoshida Takashi Utagawa Hidehiko Kumagai Hideaki Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(11):2065-2072
An inducible tryptophanase was crystallized from the cell extract of Proteus rettgeri grown in a medium containing l-tryptophan. The purification procedure included ammonium sulfate fractionation, heat treatment, DEAE-Sephadex and hydroxylapatite column chromatographies. Crystals were obtained from solutions of the purified enzyme by the addition of ammonium sulfate.The crystalline enzyme preparation was homogeneous by the criteria of ultracentrifugation and zone electrophoresis. The molecular weight was determined to be approximately 210,000.The crystalline enzyme catalyzed the degradation of l-tryptophan into indole, pyruvate and ammonia in the presence of added pyridoxal phosphate. The enzyme also catalyzed pyruvate formation from 5-hydroxy-l-tryptophan, 5-methyl-l-tryptophan, S-methyl-l-cysteine and l- cysteine. l-, d-Alanine, l-phenylalanine and indole inhibited pyruvate formation from these substrates. 相似文献
114.
Kawai Fusako Yamada Hideaki Ogata Koichi 《Bioscience, biotechnology, and biochemistry》2013,77(11):1660-1667
Some properties of pyridoxine glucoside-synthesizing enzyme were studied using the partially and highly purified enzyme preparations from Micrococcus sp. No. 431.The enzyme was stable at pH 7.0 and between 0°C and 30°C. The maximal activity was obtained at pH 8.0 and 37°C. Besides sucrose, phenyl-α-d-glucoside and maltose served as glucosyl donor. Of vitamin B6 compounds tested, only pyridoxine served as glucosyl acceptor. The enzyme activity was inhibited by PCMB and heavy metal ions, and the inhibition was prevented by 2-mercaptoethanol, indicating the enzyme would be a sulfhydryl enzyme. The activity was not affected by chelating agents and not activated by metal ions. 相似文献
115.
Haruyuki Ohkishi Daikichiro Nishikawa Hidehiko Kumagai Hideaki Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(10):2397-2398
A biofertilizer, showing antagonistic activity against potato common scab in pot tests, was produced from swine feces with a newly isolated strain, CH-33, identified as Streptomyces albidoflavus. This strain characteristically grew on fresh swine feces at 20~35°C without sterili-zation or any additives, and produced an antibiotic substance against Streptomyces scabies, the common scab-pathogen, during composting. The addition of the biofertilizer at from 0.1 g to 1.6 g total nitrogen (N) per 600 g humic volcanic ash soil in a pot did not inhibit the growth of Brassica rapa var. perviridis but increased it, even at the highest nitrogen content tested. Common scab was completely inhibited when the biofertilizer was added at 0.1 g to 1.6g as nitrogen (N) per 4 kg of scab-infected soil in a pot. Thus a biofertilizer suppressing plant pathogenic microorganisms was developed. 相似文献
116.
Morifusa Eto Lee Chee Tan Yasuyoshi Oshima Kei Takehara 《Bioscience, biotechnology, and biochemistry》2013,77(5):656-663
Alkyl phosphorothionates are isomerized to phosphorothiolates by the catalytic action of dimethylformamide. Methyl parathion (O,O-dimethyl O-p-nitrophenyl phosphorothionate) and sumithion (O,O-dimethyl O-3-methyl-4-nitrophenyl phosphorothionate) are more reactive than ethyl parathion (O,O-diethyl O-p-nitrophenyl phosphorothionate). Saligenin cyclic methyl phosphorothionate (salithion) decomposed to give a complicated pattern of products on thin layer chromatography. Besides S-methyl isomer, desmethyl sumithion (O-methyl O-3-methyl-4-nitrophenyl hydrogen phosphorothioate), 3-methyl-4-nitrophenol, methyl formate and dimethylamine were detected as reaction products from sumithion. Some other carboxylic amides including dimethylacetamide, acetamide and urea are also active. A reaction mechanism is proposed. 相似文献
117.
Hideo Takeshiba Teruomi Jojima Shinjiro Yamamoto Hideaki Tsuji 《Bioscience, biotechnology, and biochemistry》2013,77(6):1177-1183
Forty-three 3-pyridazinyl phosphorothioates were evaluated for insecticidal and acaricidal activities against two-spotted spider mite, turnip aphid, smaller brown planthopper, mosquito larvae and American cockroach. Approximate acute oral toxicity of these compounds in mice was also examined. In general, the toxicity in mice was in parallel with the pesticidal activity, but a few compounds clearly showed a high degree of selectivity between pests and mice. Especially O,O-dimethyl O-(6-cyclohexyloxy-3-pyridazinyl)phosphorothioate showed substantially reduced mammalian toxicity but maintained high insecticidal and acaricidal activity. 相似文献
118.
Tomohisa Nagasaki Masanori Sugita Hideaki Fukawa Hsin-Tung Lin 《Bioscience, biotechnology, and biochemistry》2013,77(2):363-369
Taxonomical investigation was performed on the bacterium, strain NB 320 isolated from soil, and it was identified as Enterobacter cloacae. This bacterium produced the enzyme which catalyzed the transamination reaction between 3,4-dihydroxyphenyl pyruvate and an amino acid to form l-Dopa.The optimum culture conditions for the enzyme production were studied along with the characteristics of the enzyme. The enzyme of the strain was different in some properties from that of Alcaligenes faecalis IAM 1015 which had been already studied. The former utilized glutamate as an amino donor best among the amino acids tested for transamination and was induced by the addition of glutamine and asparagine. Intact cells of the strain did not catalyze the reaction unless they were treated with sonication or with a detergent. 相似文献
119.
Hidehiko Kumagai Nobukazu Kashima Hiroshi Torii Hideaki Yamada Hitoshi Enei Shinji Okumuea 《Bioscience, biotechnology, and biochemistry》2013,77(3):472-482
Crystalline tyrosine phenol lyase was prepared from the cell extract of Erwinia herbicola grown in a medium supplemented with l-tyrosine. The crystalline enzyme was homogeneous by the criteria of ultracentrifugation and acrylamide gel electrophoresis. The molecular weight was determined to be approximately 259,000. The crystalline enzyme catalyzed the conversion of l-tyrosine into phenol, pyruvate and ammonia, in the presence of added pyridoxal phosphate. The enzyme also catalyzed pyruvate formation from d-tyrosine, S-methyl-l-cysteine, 3, 4-dihydroxyphenyl-l-alanine, l- and d-serine, and l- and d-cysteine, but at lower rates than from l-tyrosine. l-Phenyl-alanine, l-alanine, phenol and pyrocatechol inhibited pyruvate formation from l-tyrosine.Crystalline tyrosine phenol lyase from Erwinia herbicola is inactive in the absence of added pyridoxal phosphate. Binding of pyridoxal phosphate to the apoenzyme is accompanied by pronounced increase in absorbance at 340 and 425 mμ. The amount of pyridoxal phosphate bound to the apoenzyme was determined by equilibrium dialysis to be 2 moles per mole of enzyme. Addition of the substrate, l-tyrosine, or the competitive inhibitors, l-alanine and l-phenyl-alanine, to the holoenzyme causes appearance of a new absorption peak near 500 mμ which disappears as the substrate is decomposed but remains unchanged in the presence of the inhibitor. 相似文献
120.
Jong Min Kim Sakayu Shimizu Hideaki Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(11):2811-2816
Three microorganisms that degrade creatinine and contain sarcosine oxidase were isolated from soil and identified to be Alcaligenes denitrificans subsp. denitrificans J9 and Arthrobacter spp. J5 and J11. The three soil isolates degraded creatinine only via creatine by inducibly formed creatinine amidohydrolase, creatine amidinohydrolase, and sarcosine oxidase when cultivated with creatinine as the main nitrogen source. Sarcosine dehydrogenase, creatinine deiminase, and N-carbamoylsarcosine amidohydrolase were not induced by creatinine. Other microorganisms that degrade creatinine all contain sarcosine dehydrogenase as the enzyme for sarcosine oxidation, so these isolates seem to be unique in having sarcosine oxidase involved in their processes of creatinine degradation. Sarcosine oxidase was purified from A. denitrificans subsp. denitrificans J9 and partially characterized. 相似文献