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211.
The population of HLA-A2-positive individuals, currently considered serologically homogeneous, can be divided into three subtypes on the basis of antigen recognition by various HLA-A2-specific cytotoxic T lymphocytes (CTLs). When these three types of HLA-A2 antigens were analyzed biochemically, they were found to be distinct. Isoelectric focusing (IEF) of HLA antigens digested with neuraminidase (NANAse) suggested that the difference(s) reside in the polypeptide backbone of the HLA-A2 heavy chain. Biochemical analysis distinguishes three distinct categories of HLA-A2 antigens: (1) a major subtype, designated HLA-A2.I, (2) a minor subtype, designated HLA-A2.II, possessing a more basic isoelectric point (IEP) and (3) a minor HLA-A2 subtype more acidic in its IEP than HLA-A2.I, designated HLA-A2.III. A fourth HLA-A2 subtype could be defined by discordance between cell-mediated lympholysis (CML) typing and biochemical analysis. The latter HLA-A2 antigen was defined as a variant by CTL, but was biochemically indistinguishable from the major subtype HLA-A2.I. 相似文献
212.
213.
Martien van Hoof Jan Peter de Geus Marleen Roos Caroline Brown Heinz Jacobs Hidde Ploegh 《Immunogenetics》1989,30(5):330-334
The sequence of a feline class I pseudogene and its comparison with class I genes from other species is presented. The gene
isolated is a pseudogene because of the presence of four stop codons and two frame shift mutations in the first-and second-domain
encoding exons, as well as a mutation in a splice acceptor site in the third intron. By sequence comparison with the other
class I sequences determined to date, theFLA pseudogene is most closely related to theHLA-A locus products (88% nucleotide identity).
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have
been assigned the accession number M27192. 相似文献