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951.
Aya Lydie N'Dri Nadine Sellier Michèle Tixier-Boichard Catherine Beaumont Sandrine Mignon-Grasteau 《遗传、选种与进化》2007,39(5):513-528
Since feed conversion ratio (FCR) is higher in slow-growing "Label Rouge" chickens than in broiler chickens, it is important to work on its improvement in this breed. However, this involves rearing animals in cages (C), an environment very different from that used for selection (in floor pens, S) and production (outdoor, E). The aim of this study was to evaluate the importance of genotype by environment (G × E) interactions between S, C, and E environments, to find the best way to select for FCR, using 2002 related animals. Growth curve parameters were estimated and body composition measured. Individual feed conversion ratios (FCR) were recorded between 8 and 10 weeks in C. The presence of G × E interactions was assessed by the genetic correlations between the same trait recorded in different environments. Moderate but significant G × E interactions were detected for carcass traits, a significant one was observed between E and S or C for growth curve parameters but none between C and S. If G × E interactions are set aside, i.e. selecting on traits recorded in C, abdominal fatness is the best indirect selection criterion for FCR but if they are taken in account then leg yield or growth curve parameters in S and growth curve parameters in E are better. 相似文献
952.
Kawasaki A Torii K Yamashita Y Nishizawa K Kanekura K Katada M Ito M Nishimoto I Terashita K Aiso S Matsuoka M 《Cellular signalling》2007,19(12):2498-2506
Frizzled-3 (Fzd3), highly expressed in both the central nervous system (CNS) and skin, plays essential roles in axonal growth and guidance during the CNS development and may be involved in maintenance of skin integrity, although its ligand remains undetermined. In this study, we demonstrate that Wnt5a specifically binds to Fzd3 in vitro and triggers phosphorylation of Akt mediated by phosphatidylinositol-3 kinase (PI3K), but not that of ERK or protein kinase C, in human primary-cultured dermal fibroblasts. We have further found that such Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal promotes integrin-mediated adhesion of human dermal fibroblasts to collagen I-coated dishes. Based on another finding that Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal was blocked by an excess amount of a recombinant Fzd3-cysteine-rich domain (CRD), but not by that of a recombinant Fzd6-CRD, it is concluded that Wnt5a is a natural ligand of Fzd3 that triggers the PI3K/Akt signal and promotes adhesion of human dermal fibroblasts. 相似文献
953.
Sadaie T Sadaie A Takada M Hamano K Ohnishi J Ohta N Matsumoto K Sadaie Y 《Bioscience, biotechnology, and biochemistry》2007,71(3):791-799
Sludge production was reduced remarkably by reducing the dissolved oxygen supply to less than 1 mg/l in the conventional wastewater treatment procedure of a food-processing factory that produced 180 m(3) of wastewater of biochemical oxygen demand (BOD) of about 1,000 mg/l daily. DNA was extracted from the sludge and subjected to PCR amplification. The PCR product was cloned into a plasmid and sequenced. Estimation of the resident bacterial distribution by 16S rDNA sequences before and after improvement of the system suggested a remarkable gradual change in the major bacterial population from Anaerolinaeceae (15.6%) to Comamonadaceae (52.3%), members of denitrifying bacteria of Proteobacteria. Although we did not directly confirm the ability of denitrification of the resulting sludge, a change in the major final electron acceptors from oxygen to nitrate might explain the reduction in sludge production in a conventional activated sludge process when the oxygen supply was limitted. 相似文献
954.
Mica Ohara-Imaizumi Kyota Aoyagi Hajime Yamauchi Masashi Yoshida Masayuki X. Mori Yamato Hida Ha Nam Tran Masamichi Ohkura Manabu Abe Yoshihiro Akimoto Yoko Nakamichi Chiyono Nishiwaki Hayato Kawakami Kazuo Hara Kenji Sakimura Shinya Nagamatsu Yasuo Mori Junichi Nakai Toshihisa Ohtsuka 《Cell reports》2019,26(5):1213-1226.e7
955.
Hongxu Dong Steven V. Green Aya Nishiwaki Toshihiko Yamada J. Ryan Stewart Martin Deuter Erik J. Sacks 《Global Change Biology Bioenergy》2019,11(5):691-705
Miscanthus ×giganteus (M×g) is an important bioenergy feedstock crop. However, biomass production of Miscanthus has been largely limited to one sterile triploid cultivar, M×g ‘1993‐1780’, which we demonstrate can have insufficient overwintering ability in temperate regions with cold winters. Key objectives for Miscanthus breeding include greater biomass yield and better adaptation to different production environments than M×g ‘1993‐1780’. In this study, we evaluated 13 M×g genotypes, including ‘1993‐1780’, in replicated field trials conducted for three years at Urbana, IL; Dixon Springs, IL; and Jonesboro, AR. Entries were phenotyped for first‐winter overwintering ability and plant hardiness (ratio of new tillers to old), yield in years 2 and 3, and first heading date, plant height, and culm number in years 1 and 2. We observed substantial variation for overwintering ability and biomass yield among the M×g genotypes tested and identified ones with better overwintering ability and/or higher biomass yield than ‘1993‐1780’. Most entries at Urbana were damaged during the first winter, whereas few or no entries were damaged at Dixon Springs or Jonesboro. However, M×g ‘Nagara’ was entirely undamaged during the first winter and produced high biomass yields at Urbana (19.7 Mg/ha in year 2 and 20.9 Mg/ha in year 3), whereas M×g ‘1993‐1780’ exhibited an overwintering loss of 29%, had severely damaged survivors (hardiness score of 25%), and reduced biomass yield (8.1 Mg/ha in year 2 and 16.2 Mg/ha in year 3), indicating that M×g ‘Nagara’ could be a better choice in hardiness zone 5 (average annual minimum air temperature of ?23.3 to ?28.9°C) or lower. In Dixon Springs, where M×g ‘1993‐1780’ was undamaged by the first winter, it yielded highest among all the entries (21.6 Mg/ha in year 3), though not significantly higher than M×g ‘Nagara’ (18.2 Mg/ha in year 3). 相似文献
956.
957.
We generated Prx1CreER-GFP transgenic mice that express tamoxifen-inducible Cre recombinase and GFP under the control of a 2.4 kb Prx1 promoter. The transgene is expressed in osteochondro progenitor cells in the developing limb buds and in a subpopulation of periosteal cells that is closely associated with the cortical bone. GFP-expressing cells isolated from the diaphyses of long bones by cell sorting express multiple markers of periosteal cells, including Prx1, Fgf18, Tenascin-W, Periostin, and Thrombospondin 2. In addition, these cells undergo chondrogenic and osteogenic differentiation in culture upon induction. Cell fate analysis using the Rosa26 LacZ reporter indicated that transgene-expressing cells give rise to some of the chondrocytes and osteoblasts in the fracture callus. Collectively, these observations strongly suggest that the transgene-expressing cells are osteochondro progenitor cells in the periosteum. The established Prx1CreER-GFP mice would offer novel approaches for analyzing the functions of periosteal cells in vitro and in vivo. 相似文献
958.
Aya Mikogai Junn Yanagisawa Kayoko Yasuzawa-Tanaka Akiko Murayama 《Biochemical and biophysical research communications》2009,390(3):591-596
We previously identified a novel protein complex, eNoSC, which senses intracellular energy status and epigenetically regulates the rDNA locus by changing the ratio between the numbers of active and silent gene clusters. eNoSC contains a novel nucleolar protein, Nucleomethylin (NML), which has a methyltransferase-like domain and binds to Lys9-dimethylated histone H3 at the rDNA locus, along with the NAD+-dependent deacetylase SIRT1 and the histone methyltransferase SUV39H. The aim of this study was to determine the role of NML in liver after partial hepatectomy (PHx). We assessed liver regeneration and lipid metabolism after PHx in wild-type (WT) and NML transgenic (NML-TG) mice. Survival rates of NML-TG mice were reduced after PHx. We found that hepatic triglyceride content in NML-TG mice remained elevated 48 h after PHx, but not delayed liver regeneration. Moreover, hepatic ATP levels in NML-TG mice were higher than that in WT 48 h after PHx. These observations suggest that NML may regulate consumption of hepatic triglyceride in liver regeneration after PHx due to storage of excess ATP. The delayed consumption of hepatic triglyceride may be the cause of reduced survival rate in NML-TG mice. 相似文献
959.
960.
Toshiyuki Takahashi Yuji Haga Toshihiro Sakamoto Minoru Moriya Osamu Okamoto Katsumasa Nonoshita Takunobu Shibata Takuya Suga Hirobumi Takahashi Tomoko Hirohashi Aya Sakuraba Akira Gomori Hisashi Iwaasa Tomoyuki Ohe Akane Ishihara Yasuyuki Ishii Akio Kanatani Takehiro Fukami 《Bioorganic & medicinal chemistry letters》2009,19(13):3511-3516
Continuing medicinal chemistry studies to identify spiropiperidine-derived NPY Y5 receptor antagonists are described. Aryl urea derivatives of a variety of spiropiperidines were tested for their NPY Y5 receptor binding affinities. Of the spiropiperidines so far examined, spiro[3-oxoisobenzofurane-1(3H),4′-piperidine] was a useful scaffold for producing orally active NPY Y5 receptor antagonists. Oral administration of 5c significantly inhibited the Y5 agonist-induced food intake in rats with a minimum effective dose of 3 mg/kg. In addition, this compound was efficacious in decreasing body weight in diet-induced obese mice. 相似文献