Xinmei Wang PhD  Mir S. Ali PhD  Carla M. R. Lacerda PhD 《Journal of cellular biochemistry》2019,120(7):11158-11171

Both aortic and mitral valves calcify in pathological conditions; however, the prevalence of aortic valve calcification is high whereas mitral valve leaflet calcification is somewhat rare. Patterns of valvular calcification may differ due to valvular architecture, but little is known to that effect. In this study, we investigated the intrinsic osteogenic differentiation potential of aortic versus mitral valve interstitial cells provided minimal differentiation conditions. For the assessment of calcification at the cellular level, we used classic inducers of osteogenesis in stem cells: β-glycerophosphate (β-Gly), dexamethasone (Dex), and ascorbate (Asc). In addition to proteomic analyses, osteogenic markers and calcium precipitates were evaluated across treatments of aortic and mitral valve cells. The combination of β-Gly, Asc, and Dex induced aortic valve interstitial cells to synthesize extracellular matrix, overexpress osteoblastic markers, and deposit calcium. However, no strong evidence showed the calcification of mitral valve interstitial cells. Mitral cells mainly responded to Asc and Dex by cell activation. These findings provide a deeper understanding of the physiological properties of aortic and mitral valves and tendencies for calcific changes within each valve type, contributing to the development of future therapeutics for heart valve diseases.  相似文献   

    

Maryam Fakhrieh  Maryam Darvish  Abdolreza Ardeshirylajimi  Mohammad Taheri  Mir Davood Omrani 《Journal of cellular biochemistry》2019,120(9):15814-15822

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Nazir Ahmad Mir  Ashutosh Ashutosh  Shahid Ahmad Shergojry  Sajad Ahmed Wani  Fayaz Ahmad Sheikh 《Biological Rhythm Research》2019,50(3):389-399

The objective of current study was to investigate the effect of induced transportation stress in goats and to seek a novel approach to minimize the transportation and seasonal effect on the physiology of goats by supplementing them with Vitamin C and Jaggery before transportation. Fifty goats were selected and divided into five groups with ten animals in each group. Group 1 served as negative control and was neither supplemented nor transported, while as goats in groups 2 to group 5 were transported for 10 hours on the day of transportation. Group 2 goats were not supplemented; group 3 goats were supplemented with Vitamin C @180 mg/kg body weight/day; group 4 goats were supplemented with jaggery @200g/day/animal; group 5 was supplemented with both vitamin C and jaggery for 5 days before transportation. Effect of transportation stress varied significantly among groups in terms of haematological, biochemical, hormonal, mineral and blood gas parameters. Transportation up to 10 hours induced stress in goats during hot dry season as represented by changes in different physiological parameters and body weight shrinkages. Supplementation of Vitamin C and Jaggery aided in reducing transportation stress individually, with variable effects on stress response parameters and there combination synergistically proved promising in alleviating transportation stress in goats.  相似文献   

    

Marzieh Piryaei  Mir Mehdi Abolghasemi  Babak Karimi 《Phytochemical analysis : PCA》2019,30(2):193-197

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Naghmeh Jafari  Bahram Falahatkar  Mir Masoud Sajjadi 《Zeitschrift fur angewandte Ichthyologie》2019,35(1):289-295

The purpose of present study was to investigate the growth performance and hematological parameters of Siberian sturgeon Acipenser baerii in three feeding strategies (satiation feeding, restricted feeding and starvation). We considered two body weights 465.75 ± 11.18 g and 250.40 ± 12 g for large fish and small fish, respectively. In a 45‐day experiment, eighteen fiberglass tanks were used for six triplicate treatments. In restricted feeding strategy, the fish were fed to 50% of satiation and starved treatments were an absolute feed deprivation throughout the experiment. At the end of 45‐day period, the hematological parameters were measured. The reduction of weight, condition factor and daily growth rate in the smaller fish was dramatically higher than the larger fish. Feed deprivation increased the rate of weight loss. Specific growth rate were negative in the large and small fish of starved treatments. The hematological results revealed no significant differences in except for the number of monocytes in larger fish. Large satiated fish showed the highest number of monocytes. In general, these strategies (restriction and deprivation) did not have any negative impacts on the hematological parameter in both sizes but respect to fish body weight, difference in total feed input between satiated and restricted treatments changed growth performance. It seems that restricted feeding (50% of satiation) in large fish was enough to achieve optimal growth while the amount of feed provided for small fish was not enough. Therefore; we can conclude that the restricted feeding is an effective strategy in rearing sturgeon at higher weight when the rearing condition is unsuitable.  相似文献   

    

Mir Zaman Hussain  Stephen K. Hamilton  G. Philip Robertson 《Global Change Biology Bioenergy》2023,15(2):254-263

Without fertilization, harvest of perennial bioenergy cropping systems diminishes soil nutrient stocks, yet the time course of nutrient drawdown has not often been investigated. We analyzed phosphorus (P) inputs (fertilization and atmospheric deposition) and outputs (harvest and leaching losses) over 7 years in three representative biomass crops—switchgrass (Panicum virgatum L.), miscanthus (Miscanthus × giganteus) and hybrid poplar trees (Populus nigra × P. maximowiczii)—as well as in no-till corn (maize; Zea mays L.) for comparison, all planted on former cropland in SW Michigan, USA. Only corn received P fertilizer. Corn (grain and stover), switchgrass, and miscanthus were harvested annually, while poplar was harvested after 6 years. Soil test P (STP; Bray-1 method) was measured in the upper 25 cm of soil annually. Harvest P removal was calculated from tissue P concentration and harvest yield (or annual woody biomass accrual in poplar). Leaching was estimated as total dissolved P concentration in soil solutions sampled beneath the rooting depth (1.25 m), combined with hydrological modeling. Fertilization and harvest were by far the dominant P budget terms for corn, and harvest P removal dominated the P budgets in switchgrass, miscanthus, and poplar, while atmospheric deposition and leaching losses were comparatively insignificant. Because of significant P removal by harvest, the P balances of switchgrass, miscanthus, and poplar were negative and corresponded with decreasing STP, whereas P fertilization compensated for the harvest P removal in corn, resulting in a positive P balance. Results indicate that perennial crop harvest without P fertilization removed legacy P from soils, and continued harvest will soon draw P down to limiting levels, even in soils once heavily P-fertilized. Widespread cultivation of bioenergy crops may, therefore, alter P balances in agricultural landscapes, eventually requiring P fertilization, which could be supplied by P recovery from harvested biomass.  相似文献   

    

Shreyoshi Guha  Selvam Sesili  Ishfaq Hassan Mir  Chinnasamy Thirunavukkarasu 《Cell biochemistry and function》2023,41(1):4-19

A metabolic problem occurs when regular functions of the body are disrupted due to an undesirable imbalance. Nonalcoholic fatty liver disease (NAFLD) is considered as one of the most common in this category. NAFLD is subclassified and progresses from lipid accumulation to cirrhosis before advancing to hepatocellular cancer. In spite of being a critical concern, the standard treatment is inadequate. Metformin, silymarin, and other nonspecific medications are used in the management of NAFLD. Aside from this available medicine, maintaining a healthy lifestyle has been emphasized as a means of combating this. Epigenetics, which has been attributed to NAFLD, is another essential feature of this disease that has emerged as a result of several sorts of research. The mechanisms by which DNA methylation, noncoding RNA, and histone modification promote NAFLD have been extensively researched. Another organelle, mitochondria, which play a pivotal role in biological processes, contributes to the global threat. Individuals with NAFLD have been documented to have a multitude of alterations and malfunctioning. Mitochondria are mainly concerned with the process of energy production and regulation of the signaling pathway on which the fate of a cell relies. Modulation of mitochondria leads to elevated lipid deposition in the liver. Further, changes in oxidation states result in an impaired balance between the antioxidant system and reactive oxygen species directly linked to mitochondria. Hence mitochondria have a definite role in potentiating NAFLD. In this regard, it is essential to consider the role of epigenetics as well as mitochondrial contribution while developing a medication or therapy with the desired accuracy.  相似文献   

    

Mir Hilal Ahmad  Balaram Ghosh  Moshahid Alam Rizvi  Mansoor Ali  Loveleena Kaur  Amal Chandra Mondal 《Journal of cellular physiology》2023,238(2):306-328

Neuroblastoma (NB) is one of the most common heterogeneous extracranial cancers in infancy that arises from neural crest (NC) cells of the sympathetic nervous system. The Wnt signaling pathway, both canonical and noncanonical pathway, is a highly conserved signaling pathway that regulates the development and differentiation of the NC cells during embryogenesis. Reports suggest that aberrant activation of Wnt ligands/receptors in Wnt signaling pathways promote progression and relapse of NB. Wnt signaling pathways regulate NC induction and migration in a similar manner; it regulates proliferation and metastasis of NB. Inhibiting the Wnt signaling pathway or its ligands/receptors induces apoptosis and abrogates proliferation and tumorigenicity in all major types of NB cells. Here, we comprehensively discuss the Wnt signaling pathway and its mechanisms in regulating the development of NC and NB pathogenesis. This review highlights the implications of aberrant Wnt signaling in the context of etiology, progression, and relapse of NB. We have also described emerging strategies for Wnt-based therapies against the progression of NB that will provide new insights into the development of Wnt-based therapeutic strategies for NB.  相似文献   

    

Ning Liu  Poorya Chavoshnejad  Shaoheng Li  Mir Jalil Razavi  Tianming Liu  Ramana Pidaparti  Xianqiao Wang 《Biophysical journal》2021,120(17):3697-3708

Axon bundles cross-linked by microtubule (MT) associate proteins and bounded by a shell skeleton are critical for normal function of neurons. Understanding effects of the complexly geometrical parameters on their mechanical properties can help gain a biomechanical perspective on the neurological functions of axons and thus brain disorders caused by the structural failure of axons. Here, the tensile mechanical properties of MT bundles cross-linked by tau proteins are investigated by systematically tuning MT length, axonal cross-section radius, and tau protein spacing in a bead-spring coarse-grained model. Our results indicate that the stress-strain curves of axons can be divided into two regimes, a nonlinear elastic regime dominated by rigid-body like inter-MT sliding, and a linear elastic regime dominated by affine deformation of both tau proteins and MTs. From the energetic analyses, first, the tau proteins dominate the mechanical performance of axons under tension. In the nonlinear regime, tau proteins undergo a rigid-body like rotating motion rather than elongating, whereas in the nonlinear elastic regime, tau proteins undergo a flexible elongating deformation along the MT axis. Second, as the average spacing between adjacent tau proteins along the MT axial direction increases from 25 to 125 nm, the Young’s modulus of axon experiences a linear decrease whereas with the average space varying from 125 to 175 nm, and later reaches a plateau value with a stable fluctuation. Third, the increment of the cross-section radius of the MT bundle leads to a decrease in Young’s modulus of axon, which is possibly attributed to the decrease in MT numbers per cross section. Overall, our research findings offer a new perspective into understanding the effects of geometrical parameters on the mechanics of MT bundles as well as serving as a theoretical basis for the development of artificial MT complexes potentially toward medical applications.  相似文献   

    

Nazirah Bashir  Sheikh Bilal Ahmad  Muneeb U. Rehman  Showkeen Muzamil  Rahil Razak Bhat  Manzoor ur Rahman Mir  Gamal A. Shazly  Mohamed A. Ibrahim  Gehan M. Elossaily  Abdelrahman Y. Sherif  Mohsin Kazi 《Redox report : communications in free radical research》2021,26(1):62

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