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The long interspersed element-1 (LINE-1 or L1) and Alu elements are the most abundant mobile elements comprising 21% and 11% of the human genome, respectively. Since the divergence of human and chimpanzee lineages, these elements have vigorously created chromosomal rearrangements causing genomic difference between humans and chimpanzees by either increasing or decreasing the size of genome. Here, we report an exotic mechanism, retrotransposon recombination-mediated inversion (RRMI), that usually does not alter the amount of genomic material present. Through the comparison of the human and chimpanzee draft genome sequences, we identified 252 inversions whose respective inversion junctions can clearly be characterized. Our results suggest that L1 and Alu elements cause chromosomal inversions by either forming a secondary structure or providing a fragile site for double-strand breaks. The detailed analysis of the inversion breakpoints showed that L1 and Alu elements are responsible for at least 44% of the 252 inversion loci between human and chimpanzee lineages, including 49 RRMI loci. Among them, three RRMI loci inverted exonic regions in known genes, which implicates this mechanism in generating the genomic and phenotypic differences between human and chimpanzee lineages. This study is the first comprehensive analysis of mobile element bases inversion breakpoints between human and chimpanzee lineages, and highlights their role in primate genome evolution. 相似文献
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Heui-Soo Kim Rekha V. Wadekar Osamu Takenaka Byung-Hwa Hyun Timothy J. Crow 《Journal of molecular evolution》1999,49(5):699-702
The SINE-R retroposon family has been identified by its relationship with the long terminal repeats (LTRs) of human endogenous
retrovirus class K (HERV-K) as a mobile element that has evolved recently in the human genome. Here we examined the recent
evolutionary history of this class of elements by a PCR approach to genomic DNA from the African great apes and by phylogenetic
analysis including comparison with the HERV K10 parent sequence. With primers derived from a cDNA sequence from human brain,
we identified 27 sequences from the chimpanzee and 16 from the gorilla. Phylogenetic comparisons with previously recognized
sequences from the human and from the orangutan and gibbon revealed wide overlap of elements across species, suggesting multiple
origins in the course of hominoid evolution. Two human elements SINE-R.C2 and HS307 were the furthest removed from the HERV-K10
sequence but these two elements were closely related to three elements from the chimpanzee and four elements from the gorilla.
This group of elements (our clusters 14 and 15) appears to have transposed late in hominoid evolution. One element (Ch-M16)
showed 99.1% sequence identity with the SINE-R.C2 element, which is human-specific. Thus the SINE-R family appears to have
continued to be active in transposition throughout the course of primate evolution.
Received: 12 March 1999 / Accepted: 25 May 1999 相似文献
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Several methods have been compared for the immobilization of a crude commercial pectolytic enzyme product (Pectofoetidin G3X) on derivatives of technical ion exchangers. The conditions were optimized for maximum retention of the enzyme activity and the efficiency of the hydrolysis of pectin solution was measured for all the immobilized enzyme preparations. The merits and drawbacks of the methods used are discussed. 相似文献
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