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71.
72.
Anne Winkler Denise K. Gessner Christian Koch Franz-Josef Romberg Georg Dusel Eva Herzog 《Archives of animal nutrition》2015,69(6):425-441
During the periparturient phase, cows are typically in an inflammation-like condition, and it has been proposed that inflammation associated with the induction of stress of the endoplasmic reticulum (ER) in the liver contributes to the development of fatty liver syndrome and ketosis. In the present study, the hypothesis that supplementation of dairy cows with a plant product consisting of green tea (95%) and curcuma extract (5%) rich in polyphenols attenuates inflammation and ER stress in the liver during early lactation was investigated. Twenty-seven cows were assigned to two groups, either a control group (n = 14) or a treatment group (n = 13). Both groups of cows received a total mixed ration, and the ration of the treatment group was supplemented with 0.175 g of the plant product per kg dry matter from week 3 prepartum to week 9 postpartum. Dry matter intake and energy balance during week 2 to week 9 postpartum were not different between the two groups. However, cows supplemented with the plant product had a greater amount of energy-corrected milk during week 2 to week 9 postpartum and lower concentrations of triacylglycerols and cholesterol in the liver in week 1 and week 3 postpartum than cows of the control group (p < 0.05). Cows supplemented with the plant product showed a trend towards a reduced mRNA concentration of haptoglobin (p < 0.10), while relative mRNA concentrations of eight genes of the unfolded protein response considered in the liver were not different between the two groups of cows. Relative hepatic mRNA concentration of fibroblast growth factor, a stress hormone induced by various stress conditions, was reduced at week 1 and week 3 postpartum in cows supplemented with the plant product (p < 0.05). Overall, the data of this study suggest that – although there were only minor effects on the occurrence of ER stress and inflammation – a supplementation of polyphenols might be useful to improve milk yield and prevent fatty liver syndrome in dairy cows. 相似文献
73.
74.
Daniel Nobach Manon Bourg Sibylle Herzog Hildburg Lange-Herbst Jorge A. Encarna??o Markus Eickmann Christiane Herden 《PloS one》2015,10(8)
Background
Many RNA viruses arise from animal reservoirs, namely bats, rodents and insectivores but mechanisms of virus maintenance and transmission still need to be addressed. The bicolored white-toothed shrew (Crocidura leucodon) has recently been identified as reservoir of the neurotropic Borna disease virus 1 (BoDV-1).Principal Findings
Six out of eleven wild living bicoloured white-toothed shrews were trapped and revealed to be naturally infected with BoDV-1. All shrews were monitored in captivity in a long-term study over a time period up to 600 days that differed between the individual shrews. Interestingly, all six animals showed an asymptomatic course of infection despite virus shedding via various routes indicating a highly adapted host-pathogen interaction. Infectious virus and viral RNA were demonstrated in saliva, urine, skin swabs, lacrimal fluid and faeces, both during the first 8 weeks of the investigation period and for long time shedding after more than 250 days in captivity.Conclusions
The various ways of shedding ensure successful virus maintenance in the reservoir population but also transmission to accidental hosts such as horses and sheep. Naturally BoDV-1-infected living shrews serve as excellent tool to unravel host and pathogen factors responsible for persistent viral co-existence in reservoir species while maintaining their physiological integrity despite high viral load in many organ systems. 相似文献75.
Habitat structure and anthropogenic disturbance are known to affect primate diversity and abundance. However, researchers
have focused on lowland rain forests, whereas endangered deciduous forests have been neglected. We aimed to investigate the
relationships between primate diversity and abundance and habitat parameters in 10 deciduous forest fragments southeast of
Santa Cruz, Bolivia. We obtained primate data via line-transect surveys and visual and acoustic observations. In addition,
we assessed the vegetation structure (canopy height, understory density), size, isolation time, and surrounding forest area
of the fragments. We interpreted our results in the context of the historical distribution data for primates in the area before
fragmentation and interviews with local people. We detected 5 of the 8 historically observed primate species: Alouatta caraya, Aotus azarae boliviensis, Callithrix melanura, Callicebus donacophilus, and Cebus libidinosus juruanus. Total species number and detection rates decreased with understory density. Detection rates also negatively correlated with
forest areas in the surroundings of a fragment, which may be due to variables not assessed, i.e., fragment shape, distance
to nearest town. Observations for Alouatta and Aotus were too few to conduct further statistics. Cebus and Callicebus were present in 90% and 70% of the sites, respectively, and their density did not correlate with any of the habitat variables
assessed, signaling high ecological plasticity and adaptability to anthropogenic impact in these species. Detections of Callithrix were higher in areas with low forest strata. Our study provides baseline data for future fragmentation studies in Neotropical
dry deciduous forests and sets a base for specific conservation measures. 相似文献
76.
Ikram Zaïdi Chantal Ebel Majdi Touzri Etienne Herzog Jean-Luc Evrard Anne Catherine Schmit Khaled Masmoudi Moez Hanin 《Plant molecular biology》2010,73(3):325-338
The regulation of plant signalling responses by Mitogen-Activated Protein Kinases (MAPKs)-mediated protein phosphorylation is well recognized. MAP kinase phosphatases (MKPs) are negative regulators of MAPKs in eukaryotes. We report here the identification and the characterization of TMKP1, the first wheat MKP (Triticum turgidum L. subsp. Durum). Expression profile analyses performed in two durum wheat cultivars showing a marked difference in salt and drought stress tolerance, revealed a differential regulation of TMKP1. Under salt and osmotic stress, TMKP1 is induced in the sensitive wheat variety and repressed in the tolerant one. A recombinant TMKP1 was shown to be an active phosphatase and capable to interact specifically with two wheat MAPKs (TMPK3 and TMPK6). In BY2 tobacco cells transiently expressing GFP::TMKP1, the fusion protein was localized into the nucleus. Interestingly, the deletion of the N-terminal non catalytic domain results in a strong accumulation of the truncated fusion protein in the cytoplasm. In addition, when expressed in BY2 cells, TMPK3 and TMPK6 fused to red fluorescent protein (RFP) were shown to be present predominantly in the nucleus. Surprisingly, when co-expressed with the N-terminal truncated TMKP1 fusion protein; both kinases are excluded from the nuclear compartment and accumulate in the cytoplasm. This strongly suggests that TMKP1 interacts in vivo with TMPK3 and TMPK6 and controls their subcellular localization. Taken together, our results show that the newly isolated wheat MKP might play an active role in modulating the plant cell responses to salt and osmotic stress responses. 相似文献
77.
78.
Larvae provide first evidence of successful reproduction by pallid sturgeon, Scaphirhynchus albus, in the Mississippi River 总被引:1,自引:0,他引:1
R. A. Hrabik D. P. Herzog D. E. Ostendorf M. D. Petersen 《Zeitschrift fur angewandte Ichthyologie》2007,23(4):436-443
The pallid sturgeon (Scaphirhynchus albus) was not described until 1905, when it was commonly caught by commercial fishers. This species began to decline in the early 1900s presumably because of overharvest and habitat degradation. The U.S. Fish and Wildlife Service listed S. albus as an endangered species in 1990. Because S. albus live in deep, turbid rivers that are difficult to sample, very little is known about its reproductive timing and spawning habitat. The act of spawning has never been observed in nature. Captures of wild young S. albus verifying natural reproduction are rare, the last being a 4‐year‐old fish taken in 1978. In this paper, we describe the first collection of a larval S. albus from the wild and subsequent larval collections in the Mississippi River from 1998 to 2000 using a modified slingshot balloon trawl (the Missouri Trawl) designed to capture small fishes in deep, turbulent rivers. We captured larval Scaphirhynchus spp., including verified S. albus, in association with island habitats often in heavy detritus, especially at downstream tips. We postulate that Scaphirhynchus spp. spawned at the heads of islands upstream from where we collected larvae, but we cannot be certain. The capture of larval S. albus verifies reproduction possibly from the lower Missouri River to the upper and lower Mississippi River. However, we found no evidence of recruitment of S. albus from 1998 to 2000 as we were unable to capture juveniles after 374 trawl hauls that captured over 21 735 fish. 相似文献
79.
van Echten-Deckert G Saathoff M Kirfel G Herzog V 《European journal of cell biology》2007,86(11-12):675-682
During mammalian embryogenesis the emerging epidermis is temporarily covered by an epithelial monolayer, the periderm. In chicken, a second epithelial layer, the subperiderm, located underneath the periderm develops in later embryogenesis. Together the periderm and the subperiderm are referred to as the PSP unit. The cells of the PSP unit are tightly connected by tight junctions (TJ), thereby providing the embryo with an impermeable bilayered diffusion barrier. The emerging epidermis assumes its barrier function by cornification beginning at embryonic day 17 (E17) before at E18 the PSP unit undergoes desquamation. Lipid analysis of both epithelia after their mechanical separation revealed a dramatic increase to about 100-fold values of barrier-relevant ceramides, i.e. those known to essentially contribute to the diffusion barrier of the cornified envelope, in the emerging epidermis between E17 and E19. In contrast, the content of barrier-relevant ceramides in the PSP unit remained at constantly low levels throughout embryogenesis. These data strongly argue in favour of different mechanisms for the barrier function of the two epithelia. TJ in the PSP unit provide the main diffusion barrier protecting the embryo until beginning of desquamation at E18. At this developmental stage the content of cornified envelope-specific ceramides is substantially elevated, thus enabling the epidermis to fulfil its function as the major diffusion barrier after desquamation of the PSP unit. The observation that barrier-relevant ceramides are formed prior to desquamation of the PSP unit points to a precisely regulated sequence in that desquamation does not occur until the lipid-based barrier of the cornified envelope is completed and suggests in addition that these lipids might be essential regulators of the interaction between the PSP unit and the emerging epidermis. 相似文献
80.
Identification of differentially activated cell-signaling networks associated with pichinde virus pathogenesis by using systems kinomics
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Bowick GC Fennewald SM Scott EP Zhang L Elsom BL Aronson JF Spratt HM Luxon BA Gorenstein DG Herzog NK 《Journal of virology》2007,81(4):1923-1933
Phosphorylation plays a key role in regulating many signaling pathways. Although studies investigating the phosphorylated forms of signaling pathways are now commonplace, global analysis of protein phosphorylation and kinase activity has lagged behind genomics and proteomics. We have used a kinomics approach to study the effect of virus infection on host cell signaling in infected guinea pigs. Delineating the host responses which lead to clearance of a pathogen requires the use of a matched, comparative model system. We have used two passage variants of the arenavirus Pichinde, used as a biosafety level 2 model of Lassa fever virus as it produces similar pathologies in guinea pigs and humans, to compare the host cell responses between infections which lead to either a mild, self-limiting infection or lethal disease. Using this model, we can begin to understand the differences in signaling events which give rise to these markedly different outcomes. By contextualizing these data using pathway analysis, we have identified key differences in cellular signaling matrices. By comparing these differentially involved networks, we have identified a number of key signaling "nodes" which show differential phosphorylations between mild and lethal infections. We believe that these nodes provide potential targets for the development of antiviral therapies by acting at the level of the host response rather than by directly targeting viral proteins. 相似文献