全文获取类型
收费全文 | 674篇 |
免费 | 59篇 |
专业分类
733篇 |
出版年
2022年 | 5篇 |
2021年 | 22篇 |
2020年 | 9篇 |
2019年 | 12篇 |
2018年 | 14篇 |
2017年 | 8篇 |
2016年 | 19篇 |
2015年 | 33篇 |
2014年 | 19篇 |
2013年 | 33篇 |
2012年 | 42篇 |
2011年 | 27篇 |
2010年 | 21篇 |
2009年 | 23篇 |
2008年 | 25篇 |
2007年 | 22篇 |
2006年 | 26篇 |
2005年 | 21篇 |
2004年 | 25篇 |
2003年 | 18篇 |
2002年 | 20篇 |
2001年 | 23篇 |
2000年 | 13篇 |
1999年 | 20篇 |
1998年 | 6篇 |
1997年 | 6篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 13篇 |
1991年 | 15篇 |
1990年 | 18篇 |
1989年 | 10篇 |
1988年 | 6篇 |
1987年 | 12篇 |
1986年 | 9篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 6篇 |
1982年 | 6篇 |
1980年 | 8篇 |
1979年 | 8篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1975年 | 7篇 |
1974年 | 9篇 |
1973年 | 5篇 |
1969年 | 4篇 |
1968年 | 5篇 |
排序方式: 共有733条查询结果,搜索用时 15 毫秒
101.
Grasslands in southeastern South America have been extensively converted to various land uses such as agriculture, threatening regional biodiversity. Active restoration has been viewed as a management alternative for recovery of degraded areas worldwide, although most studies are conducted in forests and none has evaluated the effect of active restoration of grasslands in southeastern South America. From 2015 through 2017 we monitored a federally owned tract of grassland from the beginning of the active‐restoration process. We compared the bird community in this active‐restoration area (AR) with a reference area (NG) in Pampa grasslands in southern Brazil. We sampled birds by point counts and surveyed vegetation structure in plots. Over the 3 years of active restoration, bird species richness and abundance were higher in AR (30 species, 171 individuals) than NG (22 species, 154 individuals). The species composition also differed between the two habitats. Grassland bird species were present in both AR and NG. The vegetation structure differed between AR and NG in five attributes: height, short and tall grasses, herbs, and shrubs. Since it has been found that active restoration is useful in promoting species diversity, we encourage studies of the use of long‐term restoration efforts. Our study, even on a local scale, showed a rapid recovery of the bird community in the active‐restoration compared to native grassland, and suggests the potential for recovery of the degraded grasslands of the Brazilian Pampa biome. 相似文献
102.
103.
FCRL5 Delineates Functionally Impaired Memory B Cells Associated with Plasmodium falciparum Exposure
104.
Laura Parducci Minna V?liranta J. Sakari Salonen Tiina Ronkainen Irina Matetovici Sonia L. Fontana Tiina Eskola Pertti Sarala Yoshihisa Suyama 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2015,370(1660)
We compared DNA, pollen and macrofossil data obtained from Weichselian interstadial (age more than 40 kyr) and Holocene (maximum age 8400 cal yr BP) peat sediments from northern Europe and used them to reconstruct contemporary floristic compositions at two sites. The majority of the samples provided plant DNA sequences of good quality with success amplification rates depending on age. DNA and sequencing analysis provided five plant taxa from the older site and nine taxa from the younger site, corresponding to 7% and 15% of the total number of taxa identified by the three proxies together. At both sites, pollen analysis detected the largest (54) and DNA the lowest (10) number of taxa, but five of the DNA taxa were not detected by pollen and macrofossils. The finding of a larger overlap between DNA and pollen than between DNA and macrofossils proxies seems to go against our previous suggestion based on lacustrine sediments that DNA originates principally from plant tissues and less from pollen. At both sites, we also detected Quercus spp. DNA, but few pollen grains were found in the record, and these are normally interpreted as long-distance dispersal. We confirm that in palaeoecological investigations, sedimentary DNA analysis is less comprehensive than classical morphological analysis, but is a complementary and important tool to obtain a more complete picture of past flora. 相似文献
105.
Population aging is progressing rapidly in many industrialized countries. The United States population aged 65 and over is expected to double in size within the next 25 years. In sedentary people eating Western diets aging is associated with the development of serious chronic diseases, including type 2 diabetes mellitus, cancer and cardiovascular diseases. About 80% of adults over 65 years of age have at least one chronic disease, and 50% have at least two chronic diseases. These chronic diseases are the most important cause of illness and mortality burden, and they have become the leading driver of healthcare costs, constituting an important burden for our society. Data from epidemiological studies and clinical trials indicate that many age-associated chronic diseases can be prevented, and even reversed, with the implementation of healthy lifestyle interventions. Several recent studies suggest that more drastic interventions (i.e. calorie restriction without malnutrition and moderate protein restriction with adequate nutrition) may have additional beneficial effects on several metabolic and hormonal factors that are implicated in the biology of aging itself. Additional studies are needed to understand the complex interactions of factors that regulate aging and age-associated chronic disease. 相似文献
106.
A marginal population ofTettigidea lateralis was found to be polymorphic with respect to a large, mitotically stable supernumerary (B) chromosome. Male and female individuals may carry one or two B-chromosomes. In the male sex the frequency of individuals with one B was 33.8% whereas that of 2B-carriers was 2.9 %. A comparison with a small sample of female individuals suggests similar frequencies of B-chromosome carriers in the two sexes. The pycnocity cycle of the B's is virtually identical to that of the X chromosome which is always distinguishable by virtue of its larger size and other structural details. Persistent heterochromatic associations between the B and the X, which may last until metaphaseanaphase I, lead to a preferential migration of the B with the X to the same pole in male carriers of a single supernumerary. This distortional segregation of the B-chromosome may produce a differential transmission of the supernumerary to the two sexes if the various types of gametes are equally functional. Achiasmate, persistent B-B associations in 2B individuals can also cause segregation-distortion. The two supernumeraries segregate to the same pole in approximately 1/3 of the spermatocytes, but their poleward movement relative to that of the X is random. Both –B and +B individuals show only a single chiasma per individual bivalent. However, the presence of a single B raises very significantly the frequency at which the chiasma forms at the extreme distal ends of the L1-L2 and M3-M4 autosomes. The effect on recombination exerted by the supernumeraries and the possible implications of the segregation-distortion system ofT. lateralis are discussed in the light of recent studies on comparable B-chromosome polymorphisms.Research supported by N.R.C. of Canada. 相似文献
107.
We have previously described the occurrence, in breast and colon cancer extra-cellular matrix, of an oncofoetal form of collagen, OF/LB, able to induce an increase in cell proliferation and motility in the breast cancer cell line 8701-BC. It also caused an increased amount of type V collagen which appears to exert an anti-proliferative effect on the same cells. The aim of the present study was to investigate, at the proteomic level, the effect of OF/LB and type V collagens used as substrates for neoplastic cell growth. Due to the complexity of a whole proteomic profile, a subset of significant protein classes was used to assess variations in protein expression levels. For this study we adopted a multivariate statistical procedure that allows a global view of the variations induced by different growth conditions, when several variables have to be analyzed simultaneously. The results of this research indicate that in response to different growth substrates, chaperons and heat shock proteins contributed most to the dissimilarity in levels of expression of the selected protein spots. Moreover, we observed that different isoforms of the same protein showed independent levels of expression from one another in relation to the different collagen treatments. 相似文献
108.
109.
Evidence of hexaploid karyotype in shortnose sturgeon 总被引:1,自引:0,他引:1
Francesco Fontana Leonardo Congiu Vincent A Mudrak Joseph M Quattro Theodore I J Smith Kent Ware Serge I Doroshov 《Génome》2008,51(2):113-119
A karyotype analysis by several staining techniques was carried out on triplicate samples of the shortnose sturgeon, Acipenser brevirostrum. The chromosome number was found to be 2n = 372 +/- 6. A representative karyotype of 374 chromosomes was composed of 178 metacentrics/submetacentrics and 196 telocentrics/acrocentrics and microchromosomes. The signals of fluorescent in situ hybridization (FISH) with a HindIII satellite DNA probe were visible on 14 chromosomes. The signals obtained with a PstI satellite DNA probe appeared on 12 chromosomes. The FISH with a 5S rDNA probe revealed fluorescent signals on 6 chromosomes. These last results, compared with 2 signals in species with about 120 chromosomes and 4 in species with 240, support the hypothesis that A. brevirostrum is a hexaploid species, probably of hybrid origin. Based on these results, we propose a model explaining speciation events occurring in sturgeons by hybridization, genome duplication, and diploidization. 相似文献
110.
Helena Mata Carla Suertegaray Fontana Giovanni Nachtigall Maurício Marcos Ricardo Bornschein Marcelo Ferreira de Vasconcelos Sandro L. Bonatto 《Molecular phylogenetics and evolution》2009,53(2):450-462
Scytalopus and the recently erected Eleoscytalopus are among the Neotropical groups of birds whose taxonomy is most difficult to resolve given their very conservative morphology. We investigated the phylogeny and species limits of Eleoscytalopus and the eastern Scytalopus using two mitochondrial genes and two nuclear introns of multiple individuals from all species of these groups. The eastern Scytalopus are separated in three well defined clades also supported by morphological or vocal characteristics, although the relationships between these clades could not be resolved. We found several allopatric and very divergent lineages in these genera whose characteristics are consistent with species-level divergence, especially in S. speluncae. The great divergence between E. psychopompus and its sister species supports the former as a valid species. Our results corroborate the importance of the Bahia refuge as an avian center of endemism. 相似文献