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931.
932.
Long-, mid-, and short-styled clonal accessions of oca (Oxalis tuberosa) were intercrossed in a complete diallelic design. Pollen tube growth in styles was monitored in all diallelic crosses. Pollen
fertility was estimated by two tests: staining of pollen grains with aceto-carmine and detection of β-galactosidase activity
by the substrate X-Gal. The two methods of pollen fertility estimation were equally useful to detect fertility levels. Pollen
originating from short stamens had the highest fertility (85%) and pollen from long stamens had the lowest fertility (70%).
Pollen fertility was high throughout, but its degree varied with the stylar morph on which the pollen was formed. Long-styled
accessions had the highest rates of fertile pollen. Differences in pollen fertility at different anther levels in the same
style morph were also apparent. Pollen grain diameter of the six morph-anther level combinations was inversely correlated
with pollen fertility. Pollen grains from long stamens were the largest and pollen grains from short stamens were the smallest.
Neither pollen fertility nor pollen grain size had an influence on pollen tube growth in the style or on fruit and seed set.
Pollen tubes growing within the styles were inhibited at a different level for each of the 18 cross combinations in the diallel.
Although legitimate crosses had greatest pollen tube growth, some of the illegitimate inter- and intramorph crosses had equally
high scores. Of all illegitimate crosses, mid-styled seed parents had the lowest level of stylar incompatibility. Fruit and
seed set were highly correlated with the extent of pollen tube growth in the style. The number of pollen tubes entering ovules
in a flower was in good agreement with the number of seeds produced per fruit. Therefore, it is concluded that stylar incompatibility
is the major determinant of limited seed formation in oca even in the most successful legitimate cross combinations.
Received: 1 September 1999 / Revision accepted: 4 April 2000 相似文献
933.
Entomopathogenic fungi are promising alternatives to chemical insecticides. However, a major hurdle concerning the registration of these fungi as plant protection agents is the possible toxicity of secreted metabolites, especially secondary metabolites. This review summarizes data on specific secondary metabolites (destruxins, efrapeptins, oosporein, beauvericin and beauveriolides) produced by the important genera Beauveria, Metarhizium and Tolypocladium . The quantities of secondary metabolites produced by these fungi in vivo are usually much less than those secreted in nutrient rich liquid media. Methods and strategies are suggested which could standardize the risk assessment of fungal biological control agents. 相似文献
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936.
Klaus Hermann M. Ian Phillips Ulrich Hilgenfeldt Mohan K. Raizada 《Journal of neurochemistry》1988,51(2):398-405
This study focuses on the ability of primary rat brain cells in culture to synthesize angiotensinogen, angiotensin I, and angiotensin II. HPLC in combination with radioimmunoassay was used to characterize these compounds. Following incubation with 3H-labeled isoleucine, radioactively labeled angiotensinogen with an approximate molecular weight of 25,000 was identified in both glial and neuronal cells. Other molecular weight forms of angiotensinogen with molecular weights of about 300 and 160,000 were present in both cell types. In addition to angiotensinogen, radioactively labeled angiotensin I and angiotensin II were also synthesized by neuronal and glial cells. These results suggest that glial and neuronal cells can synthesize angiotensinogen, angiotensin I, and angiotensin II in a similar manner shown for the peripheral renin angiotensin system. 相似文献
937.
Marie-Therese Kuhnert Stephan Bialonski Nina Noennig Heinke Mai Hermann Hinrichs Christoph Helmstaedter Klaus Lehnertz 《PloS one》2013,8(11)
Learning- and memory-related processes are thought to result from dynamic interactions in large-scale brain networks that include lateral and mesial structures of the temporal lobes. We investigate the impact of incidental and intentional learning of verbal episodic material on functional brain networks that we derive from scalp-EEG recorded continuously from 33 subjects during a neuropsychological test schedule. Analyzing the networks'' global statistical properties we observe that intentional but not incidental learning leads to a significantly increased clustering coefficient, and the average shortest path length remains unaffected. Moreover, network modifications correlate with subsequent recall performance: the more pronounced the modifications of the clustering coefficient, the higher the recall performance. Our findings provide novel insights into the relationship between topological aspects of functional brain networks and higher cognitive functions. 相似文献
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939.
Analysis of motor performance variability in tasks with redundancy affords insight about synergies underlying central nervous system (CNS) control. Preferential distribution of variability in ways that minimally affect task performance suggests sophisticated neural control. Unfortunately, in the analysis of variability the choice of coordinates used to represent multi-dimensional data may profoundly affect analysis, introducing an arbitrariness which compromises its conclusions. This paper assesses the influence of coordinates. Methods based on analyzing a covariance matrix are fundamentally dependent on an investigator''s choices. Two reasons are identified: using anisotropy of a covariance matrix as evidence of preferential distribution of variability; and using orthogonality to quantify relevance of variability to task performance. Both are exquisitely sensitive to coordinates. Unless coordinates are known a priori, these methods do not support unambiguous inferences about CNS control. An alternative method uses a two-level approach where variability in task execution (expressed in one coordinate frame) is mapped by a function to its result (expressed in another coordinate frame). An analysis of variability in execution using this function to quantify performance at the level of results offers substantially less sensitivity to coordinates than analysis of a covariance matrix of execution variables. This is an initial step towards developing coordinate-invariant analysis methods for movement neuroscience. 相似文献
940.