全文获取类型
收费全文 | 410621篇 |
免费 | 36399篇 |
国内免费 | 1015篇 |
专业分类
448035篇 |
出版年
2018年 | 15762篇 |
2017年 | 14745篇 |
2016年 | 11643篇 |
2015年 | 4942篇 |
2014年 | 5239篇 |
2013年 | 7332篇 |
2012年 | 13189篇 |
2011年 | 23389篇 |
2010年 | 19890篇 |
2009年 | 15875篇 |
2008年 | 19344篇 |
2007年 | 21762篇 |
2006年 | 7348篇 |
2005年 | 8563篇 |
2004年 | 8096篇 |
2003年 | 8475篇 |
2002年 | 7828篇 |
2001年 | 11391篇 |
2000年 | 11480篇 |
1999年 | 9276篇 |
1998年 | 3629篇 |
1997年 | 3803篇 |
1996年 | 3781篇 |
1995年 | 3501篇 |
1994年 | 3513篇 |
1993年 | 3499篇 |
1992年 | 8236篇 |
1991年 | 8055篇 |
1990年 | 7816篇 |
1989年 | 7736篇 |
1988年 | 7292篇 |
1987年 | 7252篇 |
1986年 | 6696篇 |
1985年 | 6850篇 |
1984年 | 5784篇 |
1983年 | 5208篇 |
1982年 | 4160篇 |
1981年 | 3993篇 |
1980年 | 3622篇 |
1979年 | 5974篇 |
1978年 | 4691篇 |
1977年 | 4481篇 |
1976年 | 4278篇 |
1975年 | 4619篇 |
1974年 | 5071篇 |
1973年 | 4977篇 |
1972年 | 5241篇 |
1971年 | 4765篇 |
1970年 | 3683篇 |
1969年 | 3703篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Pedal progression of the slug Limax maximus was studied to obtain relations between wave velocity on the sole of the foot, wave frequency, the advance due to a single wave, and the velocity of vertically upward creeping. Each of the first three quantities is directly proportional to the simultaneous velocity of progression. Under comparable conditions, that is when work is done at a constant rate, the frequency of pedal waves is influenced by the temperature according to the equation of Arrhenius, with µ = 10,700 (Q
10 for 11° to 21° = 2.1). The velocity of a single wave must have very nearly the same "temperature characteristic," which is found also in another case of nerve net transmission (in Renilla). 相似文献
992.
993.
994.
A kinin-directed monoclonal antibody to kininogens has been developed by the fusion of murine myeloma cells with mouse splenocytes immunized with bradykinin-conjugated hemocyanin. The hybrid cells were screened by an enzyme-linked immunosorbent assay (ELISA) and a radioimmunoassay (RIA) for the secretion of antibodies to bradykinin. Ascitic fluids were produced and purified by a bradykinin-agarose affinity column. The monoclonal antibody (IgG1) bound to bradykinin, Lys-bradykinin, Met-Lys-bradykinin, and kininogens in ELISA. Further, this target-directed monoclonal antibody recognized purified low and high molecular weight bovine, human, or rat kininogens and T-kininogen in Western blotting. After turpentine-induced acute inflammation, rat kininogen levels increased dramatically in liver and serum as well as in the perfused pituitary, heart, lung, kidney, thymus, and other tissues, as identified by the kinin-directed kininogen antibody in Western blot analyses. The results were confirmed by measuring kinin equivalents of kininogens with a kinin RIA. During an induced inflammatory response, rat kininogens were localized immunohistochemically with the kinin-directed monoclonal antibody in parenchymal cells of liver, in acinar cells and some granular convoluted tubules of submandibular gland, and in the collecting tubules of kidney. Northern and cytoplasmic dot blot analyses using a kinin oligonucleotide probe showed that kininogen mRNA levels in liver but not in other tissues increase after turpentine-induced inflammation. The results indicated that rat kininogens are distributed in various tissues in addition to liver and only liver kininogen is induced by acute inflammation. The target-directed kininogen monoclonal antibody is a useful reagent for studying the structure, localization, and function of kininogens or any protein molecule containing the kinin moiety. 相似文献
995.
The Chlamydomonas cell wall degrading enzyme, lysin, acts on two substrates within the framework of the wall 总被引:5,自引:3,他引:2
The Chlamydomonas cell wall is a multilayered, extracellular matrix containing 20-25 proteins and glycoproteins, many of which are highly enriched in hydroxyproline. 80-90% of the wall protein is located in a crystalline portion of the wall that is soluble in sarkosyl-urea solutions as well as in chaotropic salts. Although the wall has no cellulose it contains a noncrystalline, highly insoluble framework portion that is responsible for the integrity and overall shape of the wall. In the present report we show that the framework of the wall is composed of two components that are acted upon by lysin, a wall degrading enzyme released by mating gametes. One, which makes up the major portion of the framework, is insoluble upon boiling in SDS-PAGE sample buffer. Lysin treatment of this portion leads to its physical degradation and the concomitant appearance of several SDS-dithiothreitol-soluble polypeptides ranging in relative molecular mass from greater than 400,000 to less than 60,000. The second component is the flagellar collar. This hollow cylinder composed of striated fibers aligned in parallel array serves as the tunnel in the wall through which the flagella protrude. Our evidence indicates that the primary collar polypeptide is a 225,000-Mr molecule that itself has at least two functional domains. One domain, contained in a 185,000-Mr fragment, permits the self-association of the molecules to form the main body of the collar. The second part of the molecule anchors the collar to the wall framework via sarkosyl-urea-insensitive, SDS-dithiothreitol-sensitive linkages. 相似文献
996.
997.
Albert R. K.; Lamm W. J.; Henderson W. R.; Bolin R. W. 《Journal of applied physiology》1989,66(1):458-464
Leukotrienes (LTs) C4 and D4 are vasoconstrictors and are thought to increase both systemic and pulmonary vascular permeability. However, we and others have observed that LTC4 and LTD4 cause pulmonary vasoconstriction but do not increase the fluid filtration coefficient of excised guinea pig lungs perfused with a cell-depleted perfusate. To determine what vascular segments were exposed to an LT-induced increase in intravascular hydrostatic pressure we measured pulmonary arterial (Ppa), pulmonary arterial occlusion (Po,a), venous (Po,v) and double occlusion (Pdo) pressures in isolated guinea pig lungs perfused with a cell-depleted buffered salt solution before and after injecting 4 micrograms of LTB4, LTC4, or LTD4 into the pulmonary artery. All three LTs increased airway pressures and also increased Ppa, Po,a, and Pdo. Histamine (15 micrograms) as well as serotonin (20 or 200 micrograms) had the same effect. In excised rabbit lungs, histamine and serotonin increased only Ppa, and Po,a. LTC4 had no vasoactivity. There are marked species variations with regard to the activity and site of action of histamine, serotonin, and LTC4 on the pulmonary circulation. 相似文献
998.
999.
1000.