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排序方式: 共有221条查询结果,搜索用时 15 毫秒
91.
Housa D Vernerová Z Herácek J Procházka B Cechák P Kuncová J Haluzík M 《Physiological research / Academia Scientiarum Bohemoslovaca》2008,57(3):451-458
Serum levels of adiponectin were measured in patients with benign prostatic hyperplasia and prostate cancer of pT2 and pT3 stage. Adiponectin ELISA assay, immunohistochemistry, and selected metabolic and biochemical parameters measurement was performed in 25 patients with benign prostatic hyperplasia and 43 with prostate cancer (17 patients with organ-confined and 26 patients with locally advanced disease). Serum adiponectin levels did not differ between prostate benign hyperplasia and cancer clinical stage T2, but was significantly higher in pT3 relative to pT2 group (14.51+/-4.92 vs. 21.41+/-8.12, P = 0.003). Tissue immunohistochemistry showed enhanced staining in neoplastic prostate glands and intraepithelial neoplasia relative to benign prostatic hyperplasia without distinction between disease grade and stage. Serum adiponectin levels are higher in locally advanced relative to organ-confined prostate cancer and may thus serve as an auxiliary marker providing further improvement for discrimination between pT2 and pT3 stages. 相似文献
92.
Kim HJ Nahm SH Lee HR Yoon GB Kim KT Kang BC Choi D Kweon OY Cho MC Kwon JK Han JH Kim JH Park M Ahn JH Choi SH Her NH Sung JH Kim BD 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,118(1):15-27
Phytophthora capsici Leonian, an oomycete pathogen, is a serious problem in pepper worldwide. Its resistance in pepper is controlled by quantitative trait loci (QTL). To detect QTL associated with P. capsici resistance, a molecular linkage map was constructed using 100 F(2) individuals from a cross between Capsicum annuum 'CM334' and C. annuum 'Chilsungcho'. This linkage map consisted of 202 restriction fragment length polymorphisms (RFLPs), 6 WRKYs and 1 simple sequence repeat (SSR) covering 1482.3 cM, with an average interval marker distance of 7.09 cM. QTL mapping of Phytophthora root rot and damping-off resistance was performed in F(2:3) originated from a cross between resistant Mexican landrace C. annuum 'CM334' and susceptible Korean landrace C. annuum 'Chilsungcho' using composite interval mapping (CIM) analysis. Four QTL explained 66.3% of the total phenotypic variations for root rot resistance and three 44.9% for damping-off resistance. Of these QTL loci, two were located close to RFLP markers CDI25 on chromosome 5 (P5) and CT211A on P9. A bacterial artificial chromosome (BAC) library from C. annuum 'CM334' was screened with these two RFLP probes to obtain sequence information around the RFLP marker loci for development of PCR-based markers. CDI25 and CT211 probes identified seven and eight BAC clones, respectively. Nine positive BAC clones containing probe regions were sequenced and used for cytogenetic analysis. One single-nucleotide amplified polymorphism (SNAP) for the CDI25 locus, and two SSRs and cleaved amplified polymorphic sequence (CAPS) for CT211 were developed using sequences of the positive BAC clones. These markers will be valuable for rapid selection of genotypes and map-based cloning for resistance genes against P. capsici. 相似文献
93.
Thioacetamide induced liver damage in zebrafish embryo as a disease model for steatohepatitis 总被引:2,自引:0,他引:2
Amali AA Rekha RD Lin CJ Wang WL Gong HY Her GM Wu JL 《Journal of biomedical science》2006,13(2):225-232
Summary Steatohepatitis has recently been increasing as a cofactor influencing the progression of fibrosis, cirrhosis, adenoma and
carcinoma in liver; however, the mechanisms by which it contributes to liver injury remain uncertain. We induced steatohepatitis
in zebrafish embryos using thioacetamide (TAA). TUNEL assay revealed significant increasing of apoptosis in liver after 5 days
post fertilization and the increasing of apoptosis was observed to be associated with the up-regulation of apoptotic genes
such as, bad, bax, P-38a, caspase-3 and 8, and JNK-1. Histological sections by oil red O stain showed the accumulation of
fatty droplets which causes the pushing of the nucleus towards one side. Up-regulation of steatosis markers such as, ACC,
adiponectin, PTL, CEBP- and , SREBP-1 was also observed. Furthermore, the elevation of glutathione peroxidase in TAA treated embryos indicated that TAA
induces lipid peroxidation which leads to causes liver damage. Zebrafish has already been considered as a good human disease
model and in this context; TAA-treated zebrafish may serve as a good animal model to study the molecular pathogenesis of steatohepatitis.
Moreover, non-availability of specific drugs to prevent steatohepatitis, this animal model may serve as a powerful preclinical
platform to study the therapeutic strategies and for evaluating chemoprevention strategies for this disease. 相似文献
94.
95.
J.H. Woo J.Y. Ko E.Y. Choi J.G. Her D.M. O'Sullivan 《Biology of sport / Institute of Sport》2013,30(1):51-55
The axe kick, in Olympic style taekwondo, has been identified as the most popular scoring technique aimed to the head during full contact competition. The first purpose of this study was to identify and investigate design issues with the current World Taekwondo Federation approved chest protector. A secondary purpose was to develop a novel chest protector addressing the identified design issues and to conduct a biomechanical analysis. Fifteen male elite Taekwondo players were selected to perform three different styles of the axe kick, i.e., front, in-out, and out-in axe kick five times each for a total of 45 kicks. Two-way repeated measures ANOVA showed significant differences between the novel and existing chest protector conditions for vertical height of the toe, downward kicking foot speed, hip flexion angle and ipsilateral shoulder flexion extension range of motion (ROM) (p < 0.05). There were no significant differences between the control condition (no chest protector) and the novel chest protector condition for these variables (p > 0.05). These results indicate that the novel chest protector interferes less with both the lower and upper limbs during the performance of the axe kick and provides a more natural, free-moving alternative to the current equipment used. 相似文献
96.
Wen-Jen Lin Shu-Jiuan Her Po-Fen Chen Russel Rhei-Long Chen 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1998,714(2):211
Interference with the UV absorbance of medrogestone by endogenous steroids in plasma was prevented by reacting plasma with oxalyl chloride. The reduction of interference was effective when oxalyl chloride was in the range 10–50 μl/ml plasma. Reaction of oxalyl chloride with plasma for 10 min could reduce interference approximately 5.5-fold, and there was no significant reduction after 30 min. The limit of quantitative concentration for medrogestone in HPLC was 1 ng/ml. The standard curves were linear with the correlation coefficient greater than 0.999 in the range of 1–30 ng/ml. The coefficients of variation of both intra- and inter-day mean values were <12% and <10% of the actual values, respectively. The developed method for plasma sample preparation and the evaluated HPLC condition were further applied to an in vivo pharmacokinetic study. 相似文献
97.
Occurrence of a Novel Galactopinitol and its Changes with Other Non-Reducing Sugars during Development of Leucaena leucocephala Seeds 总被引:1,自引:0,他引:1
Chien Ching-Te; Lin Tsan-Piao; Juo Chiun-Gung; Her Guor-Rong 《Plant & cell physiology》1996,37(4):539-544
A new cyclitol which is abudant in the late developmental stagesof leucaena (Leucaena leucocephala (Lam.) de Wit) seeds wasidentified by HPLC, NMR, and GC-MS as O--D-galactopyranosyl-(1 1)-3-O-methyl-D-chiro-inositol, a new galactopinitol. Thisgalactopinitol was initially detected midway through seed developmentand increased to 10.2 mg (g DW)-1, but decreased in mature seedsto its about a half. Stachyose content increased greatly andremained the most abundant of the soluble sugars in mature seeds(25.6 mg (g DW)-1). Artifical drying at 73% relative humidityof 70 DPA immature seeds induced the accumulation of raffinose,stachyose, galactopinitol and galactinol, but the total amountsof these sugars were only about half of those found in matureseeds. Seed germination decreased following an initial increaseafter 8 d artitifical drying to a moisture content of 24%, andthis dehydration damage probably is because of underdevelopmentof seed tissue. Galactopinitol changes in a similar fashionto the oligosaccharides during the late developmental stageand dehydration experiment, implying that galactopinitol mayplay a role in desiccation tolerance of leucaena seeds.
1Contribution no. 79 of Taiwan Forestry Research Institute. 相似文献
98.
U Zor E Her T Harell G Fischer Z Naor P Braquet E Ferber N Reiss 《Biochimica et biophysica acta》1991,1091(3):385-392
The role of protein kinase C in phospholipase A2 (PLA2) activation in rat basophilic leukemia cells (RBL-2H3) and macrophages was investigated. 12-O-Tetradecanoyl phorbol 13-acetate (TPA) doubled ionomycin-induced PLA2 activity, assessed by [3H]arachidonate release. Protein kinase C inhibitors, staurosporine and K252a (100 nM) or H-7 (15 micrograms/ml) inhibited ionomycin-stimulation of PLA2 activity by 62, 75 and 80%, respectively. Down-regulation of protein kinase C by prolonged treatment with TPA inhibited Ca2(+)-ionophore A23187 or antigen-stimulation of [3H]arachidonate release by 80%. We examined whether the inhibitory effect of dexamethasone (DEX) on PLA2 activity is related to modulation of protein kinase C activity. The 50% inhibition by DEX of ionomycin elevation of [3H]arachidonate release was almost overcome by addition of TPA. The Ca2+ ionophore and antigen-induced increase in [3H]TPA binding to intact RBL cells was not impaired by DEX. However, DEX markedly reduced phosphorylation of several proteins. 1-Oleoyl-2-acetyl-glycerol (OAG) had a sustained stimulatory effect on PLA2 activity in isolated plasma membranes derived from treated bone-marrow intact mouse macrophages, while both DEX and staurosporine reduced elevated PLA2 activity by 68 and 84%, respectively. The results support an essential role for protein kinase C in regulation of PLA2 activity. 相似文献
99.
We recently cloned from a mouse 3T3 cell cDNA library a cDNA with sequence similarity to the p42mapk protein and other members of the MAP kinase family. To determine with certainty which member of the family this clone encodes, we have expressed the cDNA in COS cells and characterized the protein product. When the pSV2MAP plasmid carrying the full-length clone was transfected into COS cells, a protein of 42,000 Da was expressed. This 42 kDa protein displayed chromatographic properties indistinguishable from the endogenous p42mapk, and could be separated from the closely related pp44. In addition, upon serum stimulation, the 42 kDa protein became tyrosine-phosphorylated and enzymatically active towards the substrate myelin basic protein. We conclude that this clone codes for a functional p42mapk protein kinase. 相似文献
100.
Denitritification and denitratification in anoxic filters were performed to generate experimental data. Also, a kinetic model of denitratification that accounts for intrinsic biokinetics and hydrodynamic behavior of the biofilter is proposed. In denitritification, the simulated results are in good agreement with the experimental data; and a higher nitrite influent concentration gives a higher nitrite reduction efficiency if the denitrifying loading is kept the same. In denitratification, the intermediate nitrite tends to accumulate, and a higher denitrifying loading results in a higher nitrite effluent concentration. By inserting biological and physical parameter values into the kinetic model, the variations in distributed fractions of nitrate-reductase (f) and nitrite-reductase (1-f) with different denitrifying loadings can be estimated by fitting in experimental data. The estimated f increased with an increase in denitrifying loading, implying that a higher denitrifying loading results in a higher nitrite effluent concentration. From parametric sensitivity analyses, the parameter f is more sensitive than other biological and physical parameters. Accordingly, the proposed kinetic model of denitratification can be used to predict the treatment performance of anoxic filters appropriately. Copyright 1998 John Wiley & Sons, Inc. 相似文献