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Felix Mba Medie Renaud Vincentelli Michel Drancourt Bernard Henrissat 《Protein expression and purification》2011,75(2):172-176
Mycobacterium tuberculosis is a facultative intracellular pathogen, and the ability of this bacterium to survive and to grow inside macrophages is central to its virulence. Multiple strategies are employed by M. tuberculosis to ensure survival in macrophages, including secretion of several proteins, which are good candidates to be virulence factors, drug targets for disease intervention, and vaccine antigens. However, some M. tuberculosis secreted proteins do not appear to play any role in the growth or survival of the bacterium in its mammalian host. Among these proteins are three putative cellulose-targeting proteins encoded by the genes Rv0062, Rv1090, and Rv1987. It has been previously shown that Rv0062 encodes an active cellulase. Here we report that Rv1090 and Rv1987 also encode functional proteins. Rv1090 is able to hydrolyze barley β-glucan while Rv1987 displays cellulose-binding activity on filter paper and on microcrystalline cellulose (Avicel). Collectively, these observations point toward a unique unknown relationship between M. tuberculosis and a cellulose-containing host. We hypothesize that amoeba could be such hosts. 相似文献
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Ugo Cenci Shannon J. Sibbald Bruce A. Curtis Ryoma Kamikawa Laura Eme Daniel Moog Bernard Henrissat Eric Maréchal Malika Chabi Christophe Djemiel Andrew J. Roger Eunsoo Kim John M. Archibald 《BMC biology》2018,16(1):137
Background
The evolution of photosynthesis has been a major driver in eukaryotic diversification. Eukaryotes have acquired plastids (chloroplasts) either directly via the engulfment and integration of a photosynthetic cyanobacterium (primary endosymbiosis) or indirectly by engulfing a photosynthetic eukaryote (secondary or tertiary endosymbiosis). The timing and frequency of secondary endosymbiosis during eukaryotic evolution is currently unclear but may be resolved in part by studying cryptomonads, a group of single-celled eukaryotes comprised of both photosynthetic and non-photosynthetic species. While cryptomonads such as Guillardia theta harbor a red algal-derived plastid of secondary endosymbiotic origin, members of the sister group Goniomonadea lack plastids. Here, we present the genome of Goniomonas avonlea—the first for any goniomonad—to address whether Goniomonadea are ancestrally non-photosynthetic or whether they lost a plastid secondarily.Results
We sequenced the nuclear and mitochondrial genomes of Goniomonas avonlea and carried out a comparative analysis of Go. avonlea, Gu. theta, and other cryptomonads. The Go. avonlea genome assembly is ~?92 Mbp in size, with 33,470 predicted protein-coding genes. Interestingly, some metabolic pathways (e.g., fatty acid biosynthesis) predicted to occur in the plastid and periplastidal compartment of Gu. theta appear to operate in the cytoplasm of Go. avonlea, suggesting that metabolic redundancies were generated during the course of secondary plastid integration. Other cytosolic pathways found in Go. avonlea are not found in Gu. theta, suggesting secondary loss in Gu. theta and other plastid-bearing cryptomonads. Phylogenetic analyses revealed no evidence for algal endosymbiont-derived genes in the Go. avonlea genome. Phylogenomic analyses point to a specific relationship between Cryptista (to which cryptomonads belong) and Archaeplastida.Conclusion
We found no convincing genomic or phylogenomic evidence that Go. avonlea evolved from a secondary red algal plastid-bearing ancestor, consistent with goniomonads being ancestrally non-photosynthetic eukaryotes. The Go. avonlea genome sheds light on the physiology of heterotrophic cryptomonads and serves as an important reference point for studying the metabolic “rewiring” that took place during secondary plastid integration in the ancestor of modern-day Cryptophyceae.155.
Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens 总被引:7,自引:0,他引:7
Klosterman SJ Subbarao KV Kang S Veronese P Gold SE Thomma BP Chen Z Henrissat B Lee YH Park J Garcia-Pedrajas MD Barbara DJ Anchieta A de Jonge R Santhanam P Maruthachalam K Atallah Z Amyotte SG Paz Z Inderbitzin P Hayes RJ Heiman DI Young S Zeng Q Engels R Galagan J Cuomo CA Dobinson KF Ma LJ 《PLoS pathogens》2011,7(7):e1002137
156.
Alison R. Erickson Brandi L. Cantarel Regina Lamendella Youssef Darzi Emmanuel F. Mongodin Chongle Pan Manesh Shah Jonas Halfvarson Curt Tysk Bernard Henrissat Jeroen Raes Nathan C. Verberkmoes Claire M. Fraser Robert L. Hettich Janet K. Jansson 《PloS one》2012,7(11)
Crohn''s disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional signatures of CD in stool samples from six twin pairs that were either healthy, or that had CD in the ileum (ICD) or colon (CCD). Integration of these omics approaches revealed several genes, proteins, and pathways that primarily differentiated ICD from healthy subjects, including depletion of many proteins in ICD. In addition, the ICD phenotype was associated with alterations in bacterial carbohydrate metabolism, bacterial-host interactions, as well as human host-secreted enzymes. This eco-systems biology approach underscores the link between the gut microbiota and functional alterations in the pathophysiology of Crohn''s disease and aids in identification of novel diagnostic targets and disease specific biomarkers. 相似文献
157.
158.
Functional implications of structure-based sequence alignment of proteins in the extracellular pectate lyase superfamily. 总被引:9,自引:2,他引:7 下载免费PDF全文
Pectate lyases are plant virulence factors that degrade the pectate component of the plant cell wall. The enzymes share considerable sequence homology with plant pollen and style proteins, suggesting a shared structural topology and possibly functional relationships as well. The three-dimensional structures of two Erwinia chrysanthemi pectate lyases, C and E, have been superimposed and the structurally conserved amino acids have been identified. There are 232 amino acids that superimpose with a root-mean-square deviation of 3 A or less. These amino acids have been used to correct the primary sequence alignment derived from evolution-based techniques. Subsequently, multiple alignment techniques have allowed the realignment of other extracellular pectate lyases as well as all sequence homologs, including pectin lyases and the plant pollen and style proteins. The new multiple sequence alignment reveals amino acids likely to participate in the parallel beta helix motif, those involved in binding Ca2+, and those invariant amino acids with potential catalytic properties. The latter amino acids cluster in two well-separated regions on the pectate lyase structures, suggesting two distinct enzymatic functions for extracellular pectate lyases and their sequence homologs. 相似文献
159.
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Espagne E Lespinet O Malagnac F Da Silva C Jaillon O Porcel BM Couloux A Aury JM Ségurens B Poulain J Anthouard V Grossetete S Khalili H Coppin E Déquard-Chablat M Picard M Contamine V Arnaise S Bourdais A Berteaux-Lecellier V Gautheret D de Vries RP Battaglia E Coutinho PM Danchin EG Henrissat B Khoury RE Sainsard-Chanet A Boivin A Pinan-Lucarré B Sellem CH Debuchy R Wincker P Weissenbach J Silar P 《Genome biology》2008,9(5):R77-22