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971.
Predator–prey interactions are important evolutionary drivers of defensive behaviors, but they are usually difficult to record. This lack of data on natural history and ecological interactions of species can be overcome through museum specimens, at least for some reptiles. When facing aggressive interactions, reptile species may exhibit the defensive behavior of autotomy by losing the tail, which is also known as “urotomy”. The inspection of preserved specimens for scars of tail breakage can reveal possible ecological and biological correlates of urotomy. Herein, we investigated how the probability of urotomy in the worm lizard Amphisbaena vermicularis is affected by sex, body size, temperature, and precipitation. We found higher chances of urotomy for specimens with larger body size and from localities with warmer temperatures or lower precipitation. There was no difference in urotomy frequency between sexes. Older specimens likely faced – and survived – more predation attempts through their lifetime than smaller ones. Specimens from warmer regions might be more active both below‐ and aboveground, increasing the odds to encounter predators and hence urotomy. Probability of urotomy decreased with increased precipitation. Possibly, in places with heavier rainfall worm lizards come more frequently to the surface when galleries are filled with rainwater, remaining more exposed to efficient predators, which could result in less survival rates and fewer tailless specimens. This interesting defensive behavior is widespread in squamates, but yet little understood among amphisbaenians. The novel data presented here improve our understanding on the correlates of tail breakage and help us to interpret more tales of lost tails.  相似文献   
972.
The extracellular tannase from Emericela nidulans was immobilized on different ionic and covalent supports. The derivatives obtained using DEAE-Sepharose and Q-Sepharose were thermally stable from 60 to 75 °C, with a half life (t50) >24 h at 80 °C at pH 5.0. The glyoxyl-agarose and amino-glyoxyl derivatives showed a thermal stability which was lower than that observed for ionic supports. However, when the stability to pH was considered, the derivatives obtained from covalent supports were more stable than those obtained from ionic supports. DEAE-Sepharose and Q-Sepharose derivatives as well as the free enzyme were stable in 30 and 50 % (v/v) 1-propanol. The CNBr-agarose derivative catalyzed complete tannic acid hydrolysis, whereas the Q-Sepharose derivative catalyzed the transesterification reaction to produce propyl gallate (88 % recovery), which is an important antioxidant.  相似文献   
973.
Anthracnose, caused by Colletotrichum spp., is one of the most common diseases affecting sweet pepper and chilli pepper production worldwide, especially in tropical and subtropical zones. This disease results in severe fruit damage both pre- and postharvest. The development of resistant cultivars is the most effective strategy for disease control, which requires knowing the genetic basis of resistance. In this study, we analysed the inheritance of resistance of Capsicum annuum to Colletotrichum scovillei at two fruit development stages. The ripe and unripe fruits were inoculated by conidia suspension, and anthracnose severity was evaluated for 8 days using a score scale. It was found that the inheritance of resistance has independent expression in ripe and unripe fruits. In both cases, two main genes are responsible for resistance with polygenic effects. Genetic control, with a quantitative aspect, is more affected by dominance than the additive effects, in both fruit development stages of C. annuum.  相似文献   
974.
Brown spot is one of the most devasting and prevalent disease of rice and its control is mainly dependent on fungicide application. Therefore, this study aimed to examine the effects of Si and Mn on the development of brown spot on rice plants grown in hydroponic culture. Rice plants (cv. ‘Metica‐1’) were grown in plastic pots containing 0 or 2 mm Si (?Si and +Si treatments, respectively) with three Mn rates (0.5, 2.5 and 10 μm ). Plants were inoculated with B. oryzae 39 days after emergence. The following components of resistance were evaluated: incubation period (IP), number of lesions (NL) per cm2 of leaf area, real disease severity (RDS) and area under brown spot progress curve (AUBSPC). The content of Si and Mn in the plant tissues was also determined. Si content was significantly higher in rice tissue of plants of the +Si treatment than of the ?Si treatment regardless of the Mn rates used. The Mn rates did not affect the Si content of the rice plants. The Mn content of the rice tissues was significantly higher in the ?Si treatment than on +Si treatment, regardless of the Mn rate used. The Mn content was significantly lower at 0.5 μm Mn in comparison to the other rates for both ?Si and +Si treatments. The IP of brown spot on rice leaves significantly increased in the +Si treatment; but the Mn rates in the presence of Si had no effect on IP. In the ?Si treatment, the IP was significantly higher only at the rate of 2.5 μm . The NL, RDS and AUBSPC were significantly reduced in the +Si treatment regardless of the Mn rates. The Mn rates in the presence of Si had no effect on these components of resistance. Overall, Si dramatically impacted the development of brown spot regardless of the presence of Mn at either low or high rates. This may be useful in regions where the soil has either toxic or deficient levels of Mn and cultivars with brown spot resistance are not commercially available.  相似文献   
975.

Background

The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression.

Methods

The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells.

Results

We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR.

Conclusions

A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein.
  相似文献   
976.
A high-fat diet (HFD) is associated with reduced glucose uptake in muscle, but not in adipose tissue. In the present study, we investigated whether a HFD can modulate glucose uptake in adipose tissue by increasing signal transduction through the CAP/Cbl pathway, independently of the PI3-K/Akt pathway. Our results suggest that, in HFD, the differential regulation of insulin-induced glucose uptake between skeletal muscle and adipose tissue may, in part, be a consequence of the CAP/Cbl/C3G pathway, since the expression of CAP and Cbl, and also the activation of this pathway were increased in adipose tissue but not in muscle.  相似文献   
977.
The present study presents the morphology, histology, and the dynamics of vitellogenesis in females of the tick Amblyomma triste. The ovary in this species is of the panoistic type, therefore it lacks nurse cells. It is composed of a layer of epithelial cells that outwardly form the wall of the ovary, but also originate the pedicel, the structure that attaches the oocytes to its external margin, as well the oocytes themselves. In Amblyomma triste, the oocytes develop in four synchronic stages, which differs from the process in other tick species. The classification of the stages of the oocytes was carried out based on the presence of four morphologic characteristics: cytoplasm appearance; site of the germ vesicle; presence, quantity, and constitution of the yolk granules and presence of chorium.  相似文献   
978.
A study was conducted to define culture conditions for in vitro growth arbuscular mycorrhizal fungi (AMF) with liverworts as hosts. Lunularia cruciata (L.) Dumortier ex. Lindberg developed in vitro monoxenic mycothalli with both Glomus proliferum Dalpé & Declerck (MUCL 41827) and Glomus intraradices Schenck & Smith (MUCL 43204). AMF inoculated plants were co-cultured in plastic Petri dishes with semi-solidified medium supplemented with sucrose and grown under filtered light. Mycothalli of L. cruciata produced external hyphae and spores in quantities equivalent to those obtained with Ri T-DNA transformed root systems.  相似文献   
979.
Most mammalian chromosomes have satellite DNA sequences located at or near the centromeres, organized in arrays of variable size and higher order structure. The implications of these specific repetitive DNA sequences and their organization for centromere function are still quite cloudy. In contrast to most mammalian species, the domestic cat seems to have the major satellite DNA family (FA-SAT) localized primarily at the telomeres and secondarily at the centromeres of the chromosomes. In the present work, we analyzed chromosome preparations from a fibrosarcoma, in comparison with nontumor cells (epithelial tissue) from the same individual, by in situ hybridization of the FA-SAT cat satellite DNA family. This repetitive sequence was found to be amplified in the cat tumor chromosomes analyzed. The amplification of these satellite DNA sequences in the cat chromosomes with variable number and appearance (marker chromosomes) is discussed and might be related to mitotic instability, which could explain the exhibition of complex patterns of chromosome aberrations detected in the fibrosarcoma analyzed.  相似文献   
980.
In oxygen-sensing PAS domains, a conserved polar residue on the proximal side of the heme cofactor, usually arginine or histidine, interacts alternately with the protein in the "on-state" or the heme edge in the "off-state" but does not contact the bound ligand directly. We assessed the contributions of this residue in Bradyrhizobium japonicum FixL by determining the effects of an R206A substitution on the heme-PAS structure, ligand affinity, and regulatory capacity. The crystal structures of the unliganded forms of the R206A and wild-type BjFixL heme-PAS domains were similar, except for a more ruffled porphyrin ring in R206A BjFixL and a relaxation of the H214 residue and heme propionate 7 due to their lost interactions. The oxygen affinity of R206A BjFixL (Kd approximately 350 microM) was 2.5 times lower than that of BjFixL, and this was due to a higher off-rate constant for the R206A variant. The enzymatic activities of the unliganded "on-state" forms, either deoxy or met-R206A BjFixL, were comparable to each other and slightly lower (twofold less) than those of the corresponding BjFixL species. The most striking difference between the two proteins was in the enzymatic activities of the liganded "off-state" forms. In particular, saturation with a regulatory ligand (the Fe(III) form with cyanide) caused a >2000-fold inhibition of the BjFixL phosphorylation of BjFixJ, but a 140-fold inhibition of this catalytic activity in R206A BjFixL. Thus, in oxygen-sensing PAS domains, the interactions of polar residues with the heme edge couple the heme-binding domain to a transmitter during signal transduction.  相似文献   
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