全文获取类型
收费全文 | 3612篇 |
免费 | 327篇 |
出版年
2023年 | 16篇 |
2022年 | 19篇 |
2021年 | 62篇 |
2020年 | 38篇 |
2019年 | 47篇 |
2018年 | 71篇 |
2017年 | 54篇 |
2016年 | 82篇 |
2015年 | 200篇 |
2014年 | 191篇 |
2013年 | 250篇 |
2012年 | 288篇 |
2011年 | 293篇 |
2010年 | 179篇 |
2009年 | 148篇 |
2008年 | 233篇 |
2007年 | 216篇 |
2006年 | 217篇 |
2005年 | 180篇 |
2004年 | 160篇 |
2003年 | 137篇 |
2002年 | 148篇 |
2001年 | 51篇 |
2000年 | 50篇 |
1999年 | 57篇 |
1998年 | 56篇 |
1997年 | 26篇 |
1996年 | 24篇 |
1995年 | 30篇 |
1994年 | 33篇 |
1993年 | 27篇 |
1992年 | 32篇 |
1991年 | 38篇 |
1990年 | 25篇 |
1989年 | 40篇 |
1988年 | 17篇 |
1987年 | 30篇 |
1986年 | 13篇 |
1985年 | 9篇 |
1984年 | 16篇 |
1983年 | 12篇 |
1982年 | 9篇 |
1981年 | 10篇 |
1980年 | 6篇 |
1979年 | 10篇 |
1978年 | 6篇 |
1972年 | 6篇 |
1968年 | 6篇 |
1965年 | 6篇 |
1964年 | 7篇 |
排序方式: 共有3939条查询结果,搜索用时 265 毫秒
61.
Martina Ferraguti Sergio Magallanes Jéssica Jiménez-Peñuela Josué Martínez-de la Puente Luz Garcia-Longoria Jordi Figuerola Jaime Muriel Tamer Albayrak Staffan Bensch Camille Bonneaud Rohan H. Clarke Gábor Á. Czirják Dimitar Dimitrov Kathya Espinoza John G. Ewen Farah Ishtiaq Wendy Flores-Saavedra László Zsolt Garamszegi Olof Hellgren Dita Horakova Kathryn P. Huyvaert Henrik Jensen Asta Križanauskienė Marcos R. Lima Charlene Lujan-Vega Eyðfinn Magnussen Lynn B. Martin Kevin D. Matson Anders Pape Møller Pavel Munclinger Vaidas Palinauskas Péter L. Pap Javier Pérez-Tris Swen C. Renner Robert Ricklefs Sergio Scebba Ravinder N. M. Sehgal Manuel Soler Eszter Szöllősi Gediminas Valkiūnas Helena Westerdahl Pavel Zehtindjiev Alfonso Marzal 《Global Ecology and Biogeography》2023,32(5):809-823
Aim
The increasing spread of vector-borne diseases has resulted in severe health concerns for humans, domestic animals and wildlife, with changes in land use and the introduction of invasive species being among the main possible causes for this increase. We explored several ecological drivers potentially affecting the local prevalence and richness of avian malaria parasite lineages in native and introduced house sparrows (Passer domesticus) populations.Location
Global.Time period
2002–2019.Major taxa studied
Avian Plasmodium parasites in house sparrows.Methods
We analysed data from 2,220 samples from 69 localities across all continents, except Antarctica. The influence of environment (urbanization index and human density), geography (altitude, latitude, hemisphere) and time (bird breeding season and years since introduction) were analysed using generalized additive mixed models (GAMMs) and random forests.Results
Overall, 670 sparrows (30.2%) were infected with 22 Plasmodium lineages. In native populations, parasite prevalence was positively related to urbanization index, with the highest prevalence values in areas with intermediate urbanization levels. Likewise, in introduced populations, prevalence was positively associated with urbanization index; however, higher infection occurred in areas with either extreme high or low levels of urbanization. In introduced populations, the number of parasite lineages increased with altitude and with the years elapsed since the establishment of sparrows in a new locality. Here, after a decline in the number of parasite lineages in the first 30 years, an increase from 40 years onwards was detected.Main conclusions
Urbanization was related to parasite prevalence in both native and introduced bird populations. In invaded areas, altitude and time since bird introduction were related to the number of Plasmodium lineages found to be infecting sparrows. 相似文献62.
Henrik Printz 《Archives of microbiology》1962,42(1):64-73
Zusammenfassung Die Natur der konzentrischen Zonen in den Stämmen von Laminarien ist lange Zeit umstritten gewesen. Nachdem aber im Jahre 1926 nachgewiesen wurde, daß das gesamte vegetative Wachstum bei den nordischen, regelmäßig blatterneuernden Laminarien periodisch und synchron in allen Teilen der Pflanzen erfolgt, war damit bewiesen, daß diese Zonen wirkliche Jahresringe darstellen, die im Zusammenhang mit dem jährlichen Blattwechsel stehen.Ebenso wie die Dendrochronologie die Jahresringe der Bäume als Grundlage für forstliche Untersuchungen über Bestockung, Alter, Produktions- und Verjüngungsverhältnisse im Waldbau benutzt, kann man aus den Jahresringen der nordischen Laminarien entsprechende Rückschlüsse ziehen.Bei der stetig steigenden Nutzbarmachung und Verwendung der Meeresalgen sind derartige Gesichtspunkte von aktuellem Interesse in der Algologie geworden.Vorliegende Arbeit ist ein Versuch, eine Bestandsanalyse in phykochronologischer Regie durchzuführen mit besonderer Berücksichtigung etwaiger Schwankungen in Assoziationen von Laminaria digitata f. stenophylla hinsichtlich Repopulation, Bestockung, Wachstumsverhältnissen und anderer damit verknüpfter biologischer Fragen.Die Untersuchung wurde in den Jahren 1955–1958 an der norwegischen Westküste vorgenommen.Es sei darauf hingewiesen, daß sowohl das vegetative Wachstum als auch die Repopulation bei der genannten Alge von Jahr zu Jahr innerhalb ausgedehnten Grenzen variieren kann und daß diese Pflanze eine nur kurze Lebensdauer hat. Exemplare von mehr als 6 Jahren wurde nicht angetroffen. Ihr Bestand wird deshalb recht rasch erneuert.Professor Dr. E. G. Pringsheim zum 80. Geburtstag gewidmet. 相似文献
63.
64.
Holger Hackstein Gerhard Jahn Holger Kirchner Gregor Bein 《Histochemistry and cell biology》1996,106(2):229-234
Radioactive in situ hybridization techniques or enzymatic detection procedures of hapten-modified human cytomegalovirus (HCMV)
probes have been widely used for studying the infection of peripheral blood leukocytes with HCMV. This report describes significant
improvements in terms of signal resolution which can be obtained by applying a highly sensitive fluorescence in situ hybridization
(FISH) technique in conjunction with a large subgenomic HCMV DNA probe. Three cosmid clones spanning 119.1 kb of the HCMV
genome (230 kb) were used to construct the digoxigenin-11-dUTP-labeled probe which was found to be superior to a total HCMV
probe representing the entire genome. Crucial hybridization parameters were analyzed systematically in order to ensure optimal
resolution power and sensitivity. The protocol was successfully applied to HCMV-infected fibroblasts and peripheral blood
leukocytes of 12 transplant patients and unambiguously facilitated the precise intracellular localization of HCMV genomes
in infected cells. Because of its excellent resolution properties, accompanied by the virtual absence by any background staining,
we recommend the use of this protocol as a sensitive approach for further virological analyses of the interactions between
HCMV and peripheral blood leukocytes at the single-cell level.
Accepted: 16 February 1996 相似文献
65.
66.
67.
The dominant phosphoprotein pp65 (UL83) of human cytomegalovirus is dispensable for growth in cell culture. 总被引:16,自引:14,他引:2
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The phosphoprotein pp65 (ppUL83) of human cytomegalovirus (HCMV) is abundantly synthesized during lytic infection in cultured fibroblasts. As a major constituent of extracellular particles, it gains entry to infected cells immediately after adsorption and subsequently translocates to the cell nucleus. This efficient transport is mediated by unique nuclear localization signals. To study the function of pp65, a viral deletion mutant was constructed by replacing the pp65 gene with the bacterial neomycin phosphotransferase gene, driven by the simian virus 40 early promoter. The resulting virus, RVAd65, could be grown and selected on human fibroblasts without complementation. The deletion of the pp65 gene in RVAd65 was verified by using Southern blot and PCR analyses. The lack of expression from the gene was investigated by immunoblotting with pp65-specific monoclonal antibodies. Single-cycle growth analyses showed that RVAd65 grew to levels of infectivity comparable to those of the wild-type virus. Therefore, pp65 is nonessential for the growth of HCMV in human fibroblasts. Electron microscopy revealed no differences in the processes of virion morphogenesis, although the maturation appeared to be delayed. However, the kinetics of expression of the immediate-early genes UL122 and UL123, the early gene UL44, and the late gene UL32 were the same in RVAd65-infected cells as in wild-type virus-infected cells in immunoblot analyses. In vitro phosphorylation assays showed that some of the virion proteins were labelled to a markedly reduced extent by virion-associated kinases in RVAd65 compared with wild-type virus. We therefore conclude that although deletion of the pp65 gene does not abolish replication of HCMV, a recombinant virus lacking pp65 displays phenotypic alterations compared with wild-type virus during growth in cultured fibroblasts. 相似文献
68.
Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine. 总被引:29,自引:4,他引:25
The synaptic vesicle protein synaptobrevin (VAMP) has recently been implicated as one of the key proteins involved in exocytotic membrane fusion. It interacts with the synaptic membrane proteins syntaxin I and synaptosome-associated protein (SNAP)-25 to form a complex which precedes exocytosis [Söllner et al. (1993b) Cell, 75, 409-418]. Here we demonstrate that the majority of synaptobrevin is bound to the vesicle protein synaptophysin in detergent extracts. No syntaxin I was found in this complex when synaptophysin-specific antibodies were used for immunoprecipitation. Conversely, no synaptophysin was associated with the synaptobrevin-syntaxin I complex when syntaxin-specific antibodies were used for immunoprecipitation. Thus, the synaptobrevin pool bound to synaptophysin is not available for binding to syntaxin I and SNAP-25, and vice versa. Synaptobrevin-synaptophysin binding was also demonstrated by chemical cross-linking in isolated nerve terminals. Furthermore, recombinant synaptobrevin II efficiently bound synaptophysin and its isoform synaptoporin, but not the more distantly related synaptic vesicle protein p29. Recombinant synaptobrevin I bound with similar efficiency, whereas the non-neuronal isoform cellubrevin displayed a lower affinity towards synaptophysin. Treatment with high NaCl concentrations resulted in a dissociation of the synaptobrevin-synaptophysin complex. In addition, the interaction of synaptobrevin with synaptophysin was irreversibly abolished by low amounts of SDS, while the interaction with syntaxin I was enhanced. We conclude that synaptophysin selectively interacts with synaptobrevin in a complex which excludes the t-SNAP receptors syntaxin I and SNAP-25, suggesting a role for synaptophysin in the control of exocytosis. 相似文献
69.
DNA rearrangements in Euplotes crassus coincide with discrete periods of DNA replication during the polytene chromosome stage of macronuclear development. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Macronuclear development in Euplotes crassus begins with polytenization of micronuclear chromosomes and is accompanied by highly precise excision of DNA sequences known as internal eliminated sequences and transposon-like elements (Tecs). Quantitation of radiolabeled-precursor incorporation into DNA indicates that DNA synthesis during formation of polytene chromosomes is not continuous and occurs during two distinct periods. We demonstrate that the timing of Tec excision coincides with these replication periods and that excision can occur during both periods even at a single locus. We also show that Tec and internal eliminated sequence excisions are coincident in the second replication period, thus providing further evidence for similarity in their excision mechanism. Inhibition of DNA synthesis with hydroxyurea diminishes Tec element excision, indicating that replication is an important aspect of the excision process. 相似文献
70.
Testosterone-regulated expression of enzymes involved in steroid and aromatic hydrocarbon catabolism in Comamonas testosteroni. 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The effect of testosterone as the sole carbon source on protein expression was analyzed in Comamonas testosteroni. Testosterone simultaneously induced the expression of steroid- and aromatic hydrocarbon-catabolizing enzymes and repressed one amino acid-degrading enzyme. It is suggested that steroids play a regulative role in catabolic enzyme synthesis during adaptive growth of C. testosteroni. 相似文献