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101.
Manuelle Viguier Stéphanie Rist Fran?ois Aubin Marie-Thérèse Leccia Marie-Aleth Richard Marina Esposito-Farèse Philippe Gaudin Thao Pham Pascal Richette Daniel Wendling Jean Sibilia Florence Tubach Club Rhumatismes et Inflammation 《PloS one》2015,10(5)
Patients with inflammatory rheumatisms, such as rheumatoid arthritis, are more prone to develop skin cancers than the general population, with an additional increased incidence when receiving TNF blockers. There is therefore a need that physicians treating patients affected with inflammatory rheumatisms with TNF blockers recognize malignant skin lesions, requiring an urgent referral to the dermatologist and a potential withdrawal or modification of the immunomodulatory treatment. We aimed to demonstrate that an online training dedicated to skin tumors increase the abilities of rheumatologists to discriminate skin cancers from benign skin tumors. A nationwide randomized web-based survey involving 141 French rheumatologists was conducted. The baseline evaluation included short cases with skin lesion pictures and multiple choice questions assessing basic knowledge on skin cancers. For each case, rheumatologists had to indicate the nature of skin lesion (benign; premalignant/malignant), their level of confidence in this diagnosis (10-points Likert scale), and the precise dermatological diagnosis among 5 propositions. Different scores were established. After randomization, only one group had access to the online formation consisting in 4 e-learning modules on skin tumors, of 15 minutes each (online training group). After reevaluation, the trained and the non-trained group (control group) were compared. The primary end-point was the number of adequate diagnoses of the nature of the skin lesions. The mean number of adequate diagnosis for the benign versus premalignant/malignant nature of the lesions was higher in the online training group (13.4 vs. 11.2 points; p value <0.0001). While the other knowledge scores were also significantly higher, no statistical difference was observed on the level of self-confidence between the 2 groups. In conclusion, the online formation was effective to improve the rheumatologists’ ability to diagnose skin cancer. 相似文献
102.
103.
Jeannotte L Aubin J Bourque S Lemieux M Montaron S Provencher St-Pierre A 《Genesis (New York, N.Y. : 2000)》2011,49(3):152-159
Cre-expressing mouse lines constitute an important asset to mammalian genetics, allowing the deletion of genes in a spatio-temporal specific manner. Our study on Hox gene function in lung development has led us to use a lung endoderm-specific deletion with the Sftpc-cre mouse line expressing the Cre recombinase gene under the control of human surfactant protein C regulatory sequences. In control experiments, the Cre recombinase faithfully activated the Rosa26-lacZ reporter gene in lung epithelium. However as early as e15.5, lungs from Sftp-Cre(+) embryos showed abnormal dilated cysts. This unexpected phenotype was also observed in mice carrying the conditional lung epithelial Hoxa5 deletion, indicating some bias due to Cre deleterious effects. Excessive apoptosis, likely due to Cre toxicity, could explain the abnormal cysts. Our findings illustrate the need for appropriate control experiments and careful interpretation of data to discriminate between the phenotype due to the targeted mutation and the confounding effects of the Cre recombinase. 相似文献
104.
Emilie Bouhsira Michel Franc Henri-Jean Boulouis Philippe Jacquiet Isabelle Raymond-Letron Emmanuel Liénard 《Applied and environmental microbiology》2013,79(23):7439-7444
Bartonella henselae (Rhizobiales: Bartonellaceae) is a Gram-negative fastidious bacterium of veterinary and zoonotic importance. The cat flea Ctenocephalides felis (Siphonaptera: Pulicidae) is the main recognized vector of B. henselae, and transmission among cats and humans occurs mainly through infected flea feces. The present study documents the use of a quantitative molecular approach to follow the daily kinetics of B. henselae within the cat flea and its excreted feces after exposure to infected blood for 48 h in an artificial membrane system. B. henselae DNA was detected in both fleas and feces for the entire life span of the fleas (i.e., 12 days) starting from 24 h after initiation of the blood meal. 相似文献
105.
Masha T. van der Sande Helge Bruelheide Wayne Dawson Jürgen Dengler Franz Essl Richard Field Sylvia Haider Mark van Kleunen Holger Kreft Joern Pagel Jan Pergl Oliver Purschke Petr Pyek Patrick Weigelt Marten Winter Fabio Attorre Isabelle Aubin Erwin Bergmeier Milan Chytrý Matteo Dainese Michele De Sanctis Jaime Fagundez Valentin Golub Greg R. Guerin Alvaro G. Gutirrez Ute Jandt Florian Jansen Borja Jimnez‐Alfaro Jens Kattge Elizabeth Kearsley Stefan Klotz Koen Kramer Marco Moretti Ülo Niinemets Robert K. Peet Josep Penuelas Petr Petík Peter B. Reich Brody Sandel Marco Schmidt Maria Sibikova Cyrille Violle Timothy J. S. Whitfeld Thomas Wohlgemuth Tiffany M. Knight 《Global Ecology and Biogeography》2020,29(2):281-294
106.
Abdou Khaled Le Loc’h François Gascuel Didier Romdhane Mohamed Salah Aubin Joël Ben Rais Lasram Frida 《The International Journal of Life Cycle Assessment》2020,25(1):105-119
The International Journal of Life Cycle Assessment - The present study assesses environmental performance of seafood production by demersal trawling in Tunisia (Gulf of Gabes) in order to analyze... 相似文献
107.
Morphological versus acoustic analysis: what is the most efficient method for sexing yelkouan shearwaters Puffinus yelkouan? 总被引:1,自引:1,他引:0
Karen Bourgeois Charlotte Curé Jér?me Legrand Elena Gómez-Díaz Eric Vidal Thierry Aubin Nicolas Mathevon 《Journal of Ornithology》2007,148(3):261-269
Sexing monomorphic seabirds is particularly difficult, and available methods have various disadvantages, such as seasonal
non-applicability or stress induction. The yelkouan shearwater Puffinus yelkouan is a poorly studied seabird endemic to the Mediterranean basin. Ecological and biological data are needed for this species,
thus necessitating the development of appropriate field methods. Here, we tested two methods for sexing yelkouan shearwaters:
the classical morphological method and a new acoustic method. Morphological analysis demonstrated sexual differences in head,
leg and feather measurements, with a high degree of overlap resulting in only 87.2% accuracy. In contrast, acoustic analysis
showed that the highest value of the fundamental frequency (FC) and duration of the clear note of calls did not overlap between
males and females. Yelkouan shearwaters can be sexed with 100% accuracy by measuring FC. If FC < 678.4 Hz, the bird is female,
while if FC > 678.4 Hz, the bird is male. Other advantages of this method are its simplicity and non-invasiveness, which are
particularly important for a potentially threatened species. 相似文献
108.
109.
Christelle Mbondji-Wonje Viswanath Ragupathy Jiangqin Zhao Aubin Nanfack Sherwin Lee Judith Torimiro Phillipe Nyambi Indira K. Hewlett 《PloS one》2014,9(11)
Background
The use of CCR5 antagonists involves determination of HIV-1 tropism prior to initiation of treatment. HIV-1 tropism can be assessed either by phenotypic or genotypic methods. Genotypic methods are extensively used for tropism prediction. However, their validation in predicting tropism of viral isolates belonging to group M non-B subtypes remains challenging. In Cameroon, the genetic diversity of HIV-1 strains is the broadest reported worldwide. To facilitate the integration of CCR5 antagonists into clinical practice in this region, there is a need to evaluate the performance of genotypic methods for predicting tropism of highly diverse group M HIV-1 strains.Methods
Tropism of diverse HIV-1 strains isolated from PBMCs from Cameroon was determined using the GHOST cell assay. Prediction, based on V3 sequences from matched plasma samples, was determined using bioinformatics algorithms and rules based on position 11/25 and net charge applied independently or combined according to Delobel''s and Garrido''s rules. Performance of genotypic methods was evaluated by comparing prediction generated with tropism assigned by the phenotypic assay.Results
Specificity for predicting R5-tropic virus was high, ranging from 83.7% to 97.7% depending on the genotypic methods used. Sensitivity for X4-tropic viruses was fairly low, ranging from 33.3% to 50%. In our study, overall, genotypic methods were less able to accurately predict X4-tropic virus belonging to subtype CRF02_AG. In addition, it was found that of the methods we used the Garrido rule has the highest sensitivity rate of over 50% with a specificity of 93%.Conclusion
Our study demonstrated that overall, genotypic methods were less sensitive for accurate prediction of HIV-1 tropism in settings where diverse HIV-1 strains co-circulate. Our data suggest that further optimization of genotypic methods is needed and that larger studies to determine their utility for tropism prediction of diverse HIV-1 strains may be warranted. 相似文献110.
MaFeng Liu Yann Ferrandez Emilie Bouhsira Martine Monteil Michel Franc Henri-Jean Boulouis Francis Biville 《PloS one》2012,7(10)
Bartonella are hemotropic bacteria responsible for emerging zoonoses. These heme auxotroph alphaproteobacteria must import heme for their growth, since they cannot synthesize it. To import exogenous heme, Bartonella genomes encode for a complete heme uptake system enabling transportation of this compound into the cytoplasm and degrading it to release iron. In addition, these bacteria encode for four or five outer membrane heme binding proteins (Hbps). The structural genes of these highly homologous proteins are expressed differently depending on oxygen, temperature and heme concentrations. These proteins were hypothesized as being involved in various cellular processes according to their ability to bind heme and their regulation profile. In this report, we investigated the roles of the four Hbps of Bartonella henselae, responsible for cat scratch disease. We show that Hbps can bind heme in vitro. They are able to enhance the efficiency of heme uptake when co-expressed with a heme transporter in Escherichia coli. Using B. henselae Hbp knockdown mutants, we show that these proteins are involved in defense against the oxidative stress, colonization of human endothelial cell and survival in the flea. 相似文献