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11.
Monique L'Hostis Henri Dumon Bertrand Dorchies Florence Boisdron André Gorenflot 《Experimental & applied acarology》1995,19(4):211-220
A longitudinal survey was carried out during a 2 year period in Western France to assess the infestation level of grazing pastures byIxodes ricinus ticks. Four farms were visited once a month and each of the grazing pastures was sampled in the centre and at the border using the blanket dragging method. A total of 3562I. ricinus (34 adults, 900 nymphs and 2628 larvae) were collected and the infestation was significantly higher during the first year (p<0.0001). The infestation level byI. ricinus varied between grazing pastures and farms. Grazing pastures in the vicinity of forest were more infested than the others, all through the study. The seasonal distribution of ticks showed peaks, with low fluctuations between farms, years and stages. Tick abundance could not be related to vegetation, but only to the vicinity of woods. 相似文献
12.
We argue that the Würm III glaciation eradicated possible European populations ofS. torvicornis, and that today, a reconquest of Europe takes place on two fronts.A western wave has reached the Pyrenees, an eastern one now occupies most of eastern and northern Europe. The western route probably started in the Maghreb, the eastern one in the Levant and the Ponto-Caspian. Animals in the west had to move north by crossing, at right angles, a series of east-west oriented river valleys and progressed slowly; animals in the east could move up river valleys extending north-east, and moved quickly. Italy was not occupied, becauseS.torvicornis is a warm stenotherm, and by the time the climate had warmed sufficiently for it to reach the southern shore of the mediterranean (ca 6000 BP), the gap with Italy was probably too large for a crossing. Cold-loving species (of the generaBranchipus, Chirocephalus) conversely, and could freely flow across the Central Mediterranean at low sea-levels (ca 12000 BP), and now occur in Italy (and the rest of Europe) as well as in Northern Africa.A prediction of our hypothesis is that the pioneer populations in Spain and Central Europe should have been isolated longest. Thisis tested and confirmed by their comparative morphology, and two subspecies,S. t. torvicornis andS. t. bucheti are reinstated.A gap across the Nile Valley where onlyS. rubricaudatus seems to occur, deserves further study. 相似文献
13.
Sabine Lhernould Yannis Karamanos Patrice Lerouge Henri Morvan 《Glycoconjugate journal》1995,12(1):94-98
The peptide-N
4-(N-acetylglucosaminyl) asparagine amidase (PNGase Se) earlier described [Lhernould S., Karamanos Y., Bourgerie S., Strecker G., Julien R., Morvan H. (1992)Glycoconjugate J
9:191–97] was partially purified from culturedSilene alba cells using affinity chromatography. The enzyme is active between pH 3.0 and 6.5, and is stable in the presence of moderate concentrations of several other protein unfolding chemicals, but is readily inactivated by SDS. Although the enzyme cleaves the carbohydrate from a variety of animal and plant glycopeptides, it does not hydrolyse the carbohydrate from most of the corresponding unfolded glycoproteins in otherwise comparable conditions. The substrate specificity of this plant PNGase supports the hypothesis that this enzyme could be at the origin of the production of unconjugated N-glycans in a suspension medium of culturedSilene alba cells.Abbreviations GlcNAc
N-acetylglucosamine
- PNGase
peptide-N
4-(N-acetylglucosaminyl) asparagine amidase
- BSA
bovine serum albumin
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- TLC
thin layer chromatography
- HPAEC-PAD
High pH anion exchange chromatography-pulsed amperometric detection 相似文献
14.
Maitrot Henri Paquin Cline Lacroix Christophe Champagne Claude P. 《Biotechnology Techniques》1997,11(7):527-531
Bifidobacterium longum was immobilized in k-carrageenan/locust bean gum gel beads, and cultured in a medium containing Lactobacillus MRS broth and whey-permeate. The same beads were incubated for 5 successive batch fermentations and freeze-dried following mixing with a protective solution. Viable population in the beads increased from 8 3 10 7 to 4.7 3 10 10 cfu/g after three batch fermentations, but no further increase in viable cell population could be achieved in the last two fermentations. The freeze-dried culture contained 3 3 10 10 cfu/g with a survival rate of approximately 10%. Survival to freeze-drying of immobilized cells was as good as that of classical free-cell cultures. Stability of freeze-dried cultures during storage at minus 17, 4 and 20°C was not influenced by immobilization. 相似文献
15.
Henri Fontaine 《Geobios》1977,10(3):471-477
After a few remarks on the coral fauna in deposits near La Roquelle in Cotentin, the genus Iowaphyllum is identifiedfor the first time in France. One species (I. bohemicum) is described here; it brings new paleogeographic and stratigraphic data. 相似文献
16.
On the continental Copepod fauna of Morocco 总被引:4,自引:4,他引:0
A series of samples, collected in different parts of Morocco, contained 26 species and one subspecies of Copepoda. Ten among these are new to the fauna of Morocco and one is new to Science. Two species (Eucyclops hadjebensis Kiefer and Mixodiaptomus laciniatus atlantis Kiefer) are endemic to Morocco; Afrocyclops gibsoni Brady and Thermocyclops schuurmanae Kiefer are Ethiopan species. The former reaches its limit of Northern extent East of the Atlas mountains; the latter is a pluvial relict. The remaining species have either a cosmopolitan, circummediterranean or North-African distribution, with the exception of Cyclops abyssorum mauretaniae which is a southern element of the Eurasiatic genus Cyclops. Its taxonomical status is reviewed in some detail. Thermocyclops infrequens Kiefer is synonymized with T. schuurmanae Kiefer. It is concluded that the largest gaps in our knowledge of the Copepoda of Morocco are now in the group of the Harpacticoida.Contribution no. 14 of the biological expedition of the University of Gent (Belgium) to Morocco, July–August 1971 相似文献
17.
Summary The addition of serum to quiescent NIH 3T3 mouse cell cultures resulted in a 10- to 20-fold increase of Rb influx which was resistant to ouabain, and only a three- to fourfold activation of ouabain-sensitive Rb influx. Stimulation of the ouabain-resistant Rb influx following serum addition reached its maximum within 2 min. The stimulation of ouabain-resistant Rb influx was a result ofV
m increase while theK
m for Rb was unchanged. Ouabain-resistant Rb influx, after serum addition, was resistant to amiloride and sensitive to ethacrinic acid. Replacing chloride in the medium by NO3
–, CO3
– and CH3COO– resulted in a drastic decrease in the ouabain-resistant Rb influx. It appeared, therefore, that the ouabain-resistant Rb influx in NIH 3T3 cells was Cl–-dependent. 相似文献
18.
van Onckelen Henri A.; Caubergs Roland; de Greef Jan A. 《Plant & cell physiology》1977,18(5):1029-1040
The influence of light and endogenous plant-growth regulatorson the evolution of - and ß-amylases in cotyledonsof Phaseolus vulgaris L. was investigated. Both enzymes, whichare not present in ungerminated seeds, appear during germinationof intact seedlings or incubation of excised cotyledons. -Amylaseactivity decreases upon exposure to light. This inhibition iscorrelated with a drastic increase in chlorophyll content anda change in the endogenous gibberellin-inhibitor balance. ß-Amylaseactivity was not affected by light treatment but was by thepresence of endogenous cytokinins. (Received February 3, 1977; ) 相似文献
19.
Bacteriophage MS2 RNA: A correlation between the stability of the codon: Anticodon interaction and the choice of code words 总被引:9,自引:0,他引:9
Henri Grosjean David Sankoff Willy Min Jou Walter Fiers R. J. Cedergren 《Journal of molecular evolution》1978,12(2):113-119
Summary The non-random distribution of degenerate code words in Bacteriophage MS2 RNA can be explained partially by considerations of the stability of the codon-anticodon complex in prokaryotic systems. Supporting this hypothesis we note that wobble codons are positively selected in codons having G and/or C in the first two positions. In contrast, wobble codons are statitically less likely in codons composed of A and U in the first two positions. Analyses of nucleotides adjacent to 5 and 3 ends of codons indicate a nonrandom distribution as well. It is thus likely that some elements of RNA evolution are independent of the structural needs of the RNA itself and of the translated protein product.This work was supported by grants from the Belgian Government Actions concertées - Gekon-certéerde Acties, N.F.W.O. and F.K.F.O. as well as from le Ministère de l'éducation du Québec. A preliminary report of this work was given at the EMBO ribosome workshop, Brussels 1976 相似文献
20.
Cyst hatching in Anostraca accelerated by retinoic acid,amplified by Calcium Ionophore A23187, and inhibited by Calcium-channel blockers 总被引:1,自引:1,他引:0
Cyst hatching, under standardized conditions, of the Anostracan species Thamnocephalus platyurus and Streptocephalus dichotomus was significantly accelerated but not increased by applying the morphogen retinoic acid (RA). Cyst hatching was enhanced but not accelerated by artificially increasing the inflow of Ca2+ to the embryonic cells, using Calcium Ionophore A 23 187. Cyst hatching was accelerated and amplified, to a level in excess of the summed effects of each treatment, by a combined application of RA and ionophore. It was inhibited almost quantitatively by the Calcium-channel blockers Nifedipin and Verapamil. The significance of these findings is discussed. 相似文献