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961.
Anne?Marike?LokhorstEmail author Jerry?van?Dijk Henk?Staats Eric?van?Dijk Geert?de?Snoo 《Human ecology: an interdisciplinary journal》2010,38(1):113-122
By adopting nature conservation practices, farmers can enhance the environmental quality and biodiversity of their land. In
this exploratory study, a behavioral intervention that focused on improving Dutch farmers’ nature conservation practices was
developed and tested. This intervention was based on insights derived from social psychology and combined tailored information
and public commitment. Participating farmers were divided in three groups: one group received tailored information only, one
group received both tailored information and a public commitment manipulation, and one group served as a control. A questionnaire
measuring relevant aspects of conservation was completed before and after the intervention. Results show that tailored information
combined with public commitment making resulted in a stronger desire to engage in conservation, an increase in surface area
of non-subsidized natural habitat, and an increase in time farmers spent on conservation. The intervention affected both subsidized
and non-subsidized conservation, but the effects were stronger for non-subsidized conservation. 相似文献
962.
Micha?l Boele van Hensbroek Job C. J. Calis Kamija S. Phiri Raymond Vet Francis Munthali Rob Kraaijenhagen Henk van den Berg Brian Faragher Imelda Bates Malcolm E. Molyneux 《PloS one》2010,5(9)
Background
Severe anaemia is a major cause of morbidity and mortality in African children. The aetiology is multi-factorial, but interventions have often targeted only one or a few causal factors, with limited success.Methods and Findings
We assessed the contribution of different pathophysiological mechanisms (red cell production failure [RCPF], haemolysis and blood loss) to severe anaemia in Malawian children in whom etiological factors have been described previously. More complex associations between etiological factors and the mechanisms were explored using structural equation modelling. In 235 children with severe anaemia (haemoglobin<3.2 mMol/L [5.0 g/dl]) studied, RCPF, haemolysis and blood loss were found in 48.1%, 21.7% and 6.9%, respectively. The RCPF figure increased to 86% when a less stringent definition of RCPF was applied. RCPF was the most common mechanism in each of the major etiological subgroups (39.7–59.7%). Multiple aetiologies were common in children with severe anaemia. In the final model, nutritional and infectious factors, including malaria, were directly or indirectly associated with RCPF, but not with haemolysis.Conclusion
RCPF was the most common pathway leading to severe anaemia, from a variety of etiological factors, often found in combination. Unlike haemolysis or blood loss, RCPF is a defect that is likely to persist to a significant degree unless all of its contributing aetiologies are corrected. This provides a further explanation for the limited success of the single factor interventions that have commonly been applied to the prevention or treatment of severe anaemia. Our findings underline the need for a package of measures directed against all of the local aetiologies of this often fatal paediatric syndrome. 相似文献963.
964.
Enock Matovu Claire M. Mugasa Rosine Ali Ekangu Stijn Deborggraeve George W. Lubega Thierry Laurent Gerard J. Schoone Henk D. Schallig Philippe Büscher 《PLoS neglected tropical diseases》2010,4(7)
Background
The polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA) have been recently modified by coupling to oligochromatography (OC) for easy and fast visualisation of products. In this study we evaluate the sensitivity and specificity of the PCR-OC and NASBA-OC for diagnosis of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense human African trypanosomiasis (HAT).Methodology and Results
Both tests were evaluated in a case-control design on 143 HAT patients and 187 endemic controls from the Democratic Republic of Congo (DRC) and Uganda. The overall sensitivity of PCR-OC was 81.8% and the specificity was 96.8%. The PCR-OC showed a sensitivity and specificity of 82.4% and 99.2% on the specimens from DRC and 81.3% and 92.3% on those from Uganda. NASBA-OC yielded an overall sensitivity of 90.2%, and a specificity of 98.9%. The sensitivity and specificity of NASBA-OC on the specimens from DRC was 97.1% and 99.2%, respectively. On the specimens from Uganda we observed a sensitivity of 84.0% and a specificity of 98.5%.Conclusions/Significance
The tests showed good sensitivity and specificity for the T. b. gambiense HAT in DRC but rather a low sensitivity for T. b. rhodesiense HAT in Uganda. 相似文献965.
Rafael Henrique Nóbrega Caaj Douwe Greebe Henk van de Kant Jan Bogerd Luiz Renato de Fran?a Rüdiger W. Schulz 《PloS one》2010,5(9)
Background
Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, and reside within a specific microenvironment in the testes called “niche” which regulates stem cell properties, such as, self-renewal, pluripotency, quiescence and their ability to differentiate.Methodology/Principal Findings
Here, we introduce zebrafish as a new model for the study of SSCs in vertebrates. Using 5′-bromo-2′-deoxyuridine (BrdU), we identified long term BrdU-retaining germ cells, type A undifferentiated spermatogonia as putative stem cells in zebrafish testes. Similar to rodents, these cells were preferentially located near the interstitium, suggesting that the SSC niche is related to interstitial elements and might be conserved across vertebrates. This localization was also confirmed by analyzing the topographical distribution of type A undifferentiated spermatogonia in normal, vasa::egfp and fli::egfp zebrafish testes. In the latter one, the topographical arrangement suggested that the vasculature is important for the SSC niche, perhaps as a supplier of nutrients, oxygen and/or signaling molecules. We also developed an SSC transplantation technique for both male and female recipients as an assay to evaluate the presence, biological activity, and plasticity of the SSC candidates in zebrafish.Conclusions/Significance
We demonstrated donor-derived spermato- and oogenesis in male and female recipients, respectively, indicating the stemness of type A undifferentiated spermatogonia and their plasticity when placed into an environment different from their original niche. Similar to other vertebrates, the transplantation efficiency was low. This might be attributed to the testicular microenvironment created after busulfan depletion in the recipients, which may have caused an imbalance between factors regulating self-renewal or differentiation of the transplanted SSCs. 相似文献966.
In the present study, structural xeromorphic features in modern and subfossil Quercus laurifolia leaves from southern Florida were quantified to reconstruct past precipitation changes in sensitive terrestrial settings. Absolute cell numbers/mm(2), quantified as epidermal cell density (ED) have been analyzed on leaves from herbarium collections as well as the leaves accumulated during the past 125 years in peat deposits. The results reveal a common principal correlation between the measured ED and winter precipitation (November through March, NDJFM: Herbarium r = -0.74; peat profiles FAK98 r = -0.72, FAK02 r = -0.53) providing a measure of seasonal drought stress. In Florida, the amount of winter precipitation depends on El Ni?o-Southern Oscillation (ENSO) activity, where El Ni?o years produce wet and cold winters, while La Ni?a winters are dry and warm. The negative correlation between cell numbers and winter precipitation has the potential to record precipitation variability from subfossil leaves on near-annual to decadal time scales. In subtropical, terrestrial environments, where traditional paleo-proxies are limited, systematic analysis of leaf morphological characteristics can provide important information on precipitation changes through time. 相似文献
967.
968.
A silver spoon for a golden future: long-term effects of natal origin on fitness prospects of oystercatchers (Haematopus ostralegus) 总被引:1,自引:1,他引:0
Van de Pol M Bruinzeel LW Heg D Van der Jeugd HP Verhulst S 《The Journal of animal ecology》2006,75(2):616-626
1. Long-term effects of conditions during early development on fitness are important for life history evolution and population ecology. Using multistrata mark-recapture models on 20 years of data, we quantified the relation between rearing conditions and lifetime fitness in a long-lived shorebird, the oystercatcher (Haematopus ostralegus). We addressed specifically the relative contribution of short- and long-term effects of rearing conditions to overall fitness consequences. 2. Rearing conditions were defined by differences in natal habitat quality, in which there is a clear dichotomy in our study population. In the first year of life, fledglings from high-quality natal origin had a 1.3 times higher juvenile survival. Later in life (age 3-11), individuals of high-quality natal origin had a 1.6 times higher adult prebreeder survival. The most striking effect of natal habitat quality was that birds that were reared on high-quality territories had a higher probability of settling in high-quality habitat (44% vs. 6%). Lifetime reproductive success of individuals born in high-quality habitat was 2.2 times higher than that of individuals born in low-quality habitat. This difference increased further when fitness was calculated over several generations, due to a correlation between the quality of rearing conditions of parents and their offspring. 3. Long-term effects of early conditions contributed more to overall fitness differences as short-term consequences, contrary to common conceptions on this issue. 4. This study illustrates that investigating only short-term effects of early conditions can lead to the large underestimation of fitness consequences. We discuss how long-term consequences of early conditions may affect settlement decisions and source-sink population interactions. 相似文献
969.
Voigt CC Kelm DH Visser GH 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2006,176(3):213-222
Recently, it was argued that extrinsic factors, such as high foraging costs, lead to elevated field metabolic rates (FMR).
We tested this suggestion by comparing the FMR of nectar-feeding and fruit-eating bats. We hypothesized that the foraging
effort per energy reward is higher for nectar-feeding mammals than for fruit-eating mammals, since energy rewards at flowering
plants are smaller than those at fruiting plants. Using the doubly labelled water method, we measured the FMR of nectar-feeding
Glossophaga commissarisi and fruit-eating Carollia brevicauda, which coexisted in the same rainforest habitat and shared the same daytime roosts. Mass-specific FMR of G. commissarisi exceeded that of C. brevicauda by a factor of almost two: 5.3±0.6 kJ g−1 day−1 for G. commissarisi and 2.8±0.4 kJ g−1 day−1 for C. brevicauda. Since nectar-feeding bats imbibe nectar droplets of only 193 J energy content during each flower visit, a G. commissarisi bat has to perform several 100 flower visits per night to meet its energy requirement. The fruit-eating C. brevicauda, on the other hand, needs to harvest only 3–12 Piper infructescenses per night, as the energy reward per Piper equals ca. 6–30 kJ. We argue that the flowering and fruiting plants exert different selective forces on the foraging behaviour
and energetics of pollinators and the seed dispersers, respectively. A comparison between nectar-feeding and non-nectar-feeding
species in various vertebrate taxa demonstrates that pollinators have elevated FMRs. 相似文献
970.
Xiao B Zhong G Obayashi M Yang D Chen K Walsh MP Shimoni Y Cheng H Ter Keurs H Chen SR 《The Biochemical journal》2006,396(1):7-16
We have recently shown that RyR2 (cardiac ryanodine receptor) is phosphorylated by PKA (protein kinase A/cAMP-dependent protein kinase) at two major sites, Ser-2030 and Ser-2808. In the present study, we examined the properties and physiological relevance of phosphorylation of these two sites. Using site- and phospho-specific antibodies, we demonstrated that Ser-2030 of both recombinant and native RyR2 from a number of species was phosphorylated by PKA, indicating that Ser-2030 is a highly conserved PKA site. Furthermore, we found that the phosphorylation of Ser-2030 responded to isoproterenol (isoprenaline) stimulation in rat cardiac myocytes in a concentration- and time-dependent manner, whereas Ser-2808 was already substantially phosphorylated before beta-adrenergic stimulation, and the extent of the increase in Ser-2808 phosphorylation after beta-adrenergic stimulation was much less than that for Ser-2030. Interestingly, the isoproterenol-induced phosphorylation of Ser-2030, but not of Ser-2808, was markedly inhibited by PKI, a specific inhibitor of PKA. The basal phosphorylation of Ser-2808 was also insensitive to PKA inhibition. Moreover, Ser-2808, but not Ser-2030, was stoichiometrically phosphorylated by PKG (protein kinase G). In addition, we found no significant phosphorylation of RyR2 at the Ser-2030 PKA site in failing rat hearts. Importantly, isoproterenol stimulation markedly increased the phosphorylation of Ser-2030, but not of Ser-2808, in failing rat hearts. Taken together, these observations indicate that Ser-2030, but not Ser-2808, is the major PKA phosphorylation site in RyR2 responding to PKA activation upon beta-adrenergic stimulation in both normal and failing hearts, and that RyR2 is not hyperphosphorylated by PKA in heart failure. Our results also suggest that phosphorylation of RyR2 at Ser-2030 may be an important event associated with altered Ca2+ handling and cardiac arrhythmia that is commonly observed in heart failure upon beta-adrenergic stimulation. 相似文献