全文获取类型
收费全文 | 4133篇 |
免费 | 387篇 |
国内免费 | 239篇 |
出版年
2024年 | 5篇 |
2023年 | 47篇 |
2022年 | 126篇 |
2021年 | 211篇 |
2020年 | 123篇 |
2019年 | 161篇 |
2018年 | 176篇 |
2017年 | 130篇 |
2016年 | 172篇 |
2015年 | 284篇 |
2014年 | 287篇 |
2013年 | 342篇 |
2012年 | 357篇 |
2011年 | 346篇 |
2010年 | 206篇 |
2009年 | 176篇 |
2008年 | 235篇 |
2007年 | 194篇 |
2006年 | 152篇 |
2005年 | 160篇 |
2004年 | 116篇 |
2003年 | 118篇 |
2002年 | 88篇 |
2001年 | 84篇 |
2000年 | 69篇 |
1999年 | 69篇 |
1998年 | 42篇 |
1997年 | 38篇 |
1996年 | 37篇 |
1995年 | 38篇 |
1994年 | 27篇 |
1993年 | 20篇 |
1992年 | 24篇 |
1991年 | 24篇 |
1990年 | 17篇 |
1989年 | 9篇 |
1988年 | 11篇 |
1987年 | 12篇 |
1986年 | 7篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1959年 | 1篇 |
排序方式: 共有4759条查询结果,搜索用时 15 毫秒
11.
Immunoelectron microscopic double labeling of alkaline phosphatase and penicillinase with colloidal gold in frozen thin sections of Bacillus licheniformis 749/C. 总被引:3,自引:2,他引:1
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The subcellular distribution of alkaline phosphatase and penicillinase was determined by double labeling frozen thin sections of Bacillus licheniformis 749/C with colloidal gold-immunoglobulin G (IgG). Antipenicillinase and anti-alkaline phosphatase antibodies were used to prepare complexes with 5- and 15-nm colloidal gold particles, respectively. The character of the labeling of membrane-bound alkaline phosphatase and penicillinase was different: the immunolabels for alkaline phosphatase (15-nm particles) were bound to a few sites at the inner surface of the plasma membrane, and the gold particles formed clusters of various sizes at the binding sites; the immunolabels for penicillinase (5-nm particles), on the other hand, were bound to the plasma membrane in a dispersed and random fashion. In the cytoplasm, immunolabels for both proteins were distributed randomly, and the character of their binding was similar. The labeling was specific: pretreating the frozen thin sections with different concentrations of anti-alkaline phosphatase or penicillinase blocked the binding of the immunolabel prepared with the same antibody. Binding could be fully blocked by pretreatment with 800 micrograms of either antibody per ml. 相似文献
12.
13.
We describe the construction in retroviral vectors and the expression of recombinant rat fibronectin (FN) cDNAs corresponding with the various alternatively spliced forms of FN. In NIH 3T3 cells, the exogenous rat FN subunits are efficiently secreted as heterodimers with endogenous mouse subunits. In contrast, in lymphoid WEHI231 cells, there is no endogenous FN synthesis and the recombinant FNs are secreted and can be purified as homogeneous proteins. We show that the purified recombinant FNs are biochemically and biologically functional. In basic assays for adhesion, spreading, cytoskeletal organization, and migration using various established adherent cell lines, different forms of FNs containing the different alternatively spliced segments show no marked differences in activity. We have used these recombinant FNs to investigate three systems in which earlier results had suggested potential differences between different forms of FN. First, all forms tested appear equally active in restoring normal morphology to a transformed cell line. Second, we detect minor differences in their ability to assemble into preexisting extracellular matrices. Finally, we report that only those forms of FN that contain the V segment will promote the spreading of a lymphoid cell line indicating that this segment confers additional biological functions for some cell types, a result that confirms and extends earlier data. These homogeneous, biologically active recombinant FNs will allow further studies of the role of the alternatively spliced segments of FN. 相似文献
14.
15.
Lipid Composition in Scrapie-Infected Mouse Brain: Prion Infection Increases the Levels of Dolichyl Phosphate and Ubiquinone 总被引:1,自引:0,他引:1
Zhizhong Guan Magnus Söderberg Pavel Sindelar Stanley B. Prusiner †Krister Kristensson Gustav Dallner 《Journal of neurochemistry》1996,66(1):277-285
Abstract: The neutral and phospholipid composition of mouse brain infected with scrapie prions was investigated. During the later stages of this disease, the level of dolichol decreased by 30% whereas the level of dolichyl phosphate increased by 30%. In terminally ill mice, there was also a 2.5-fold increase in both total ubiquinone and its reduced form. Furthermore, α-tocopherol was elevated at this stage by 50%. In contrast, no changes were observed in phospholipid amount, in phospholipid composition, and in phosphatidylethanolamine plasmalogen content during the entire disease process. The fatty acid and aldehyde composition of individual phospholipids remained unaltered as well. No modifications could be detected in cholesterol content. Thus, the majority of membrane lipids in scrapie-infected mouse brain are modified in neither quantity nor structure, but specific changes occur to a few polyisoprenoid lipids. This specificity indicates that, although prions accumulate in lysosomes, the infection process is not associated with a general membrane destruction caused by lysosomal enzyme leakage. 相似文献
16.
Abstract Isolation and identification of allatostatin 4 (AST 4) from crude brain extracts of female Diploptera punctata are described. Synthetic analogues of allatostatin 4 truncated from the N-terminal were evaluated to gain some knowledge of structure-activity correlation. AST 4 reversibly inhibits the biosynthesis of JH in vitro . AST 4 has the following sequence: Asp-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 . This neurohormone (AST 4) has no sequence similarity with any known neuropeptide from other organisms. Synthetic AST 4 as well as truncation fragments, including two with the five and six amino acids terminal residues deleted showed in vitro activity distinguishable from that of the native allatostatin. 相似文献
17.
沿海防护林防海煞危害初探 总被引:2,自引:0,他引:2
通过后牧村海岸防护林带附近空气中主要盐离子飘尘的观测分析表明,林带对大气盐尘的吸附和截留作用,无林带地区平均沉降量为4.3μg.m^-3.100m^-1,通过林带时为18.4μm^-3.100m^-1。林带的吸队量与树种、枝叶形状有关,侧柏对Cl^-和Na^+的吸附量分别为6330和3350mg.kg^-1,而黑松为1570和950mg.kg^-1,空气中盐尘受天气条件特别是风速的影响,阴天,风速 相似文献
18.
七星瓢虫(Coccmella septimpunctata)为了适应环境的变化,通过咽侧体产生保幼激素的活动调节其生殖作用。为了探索内外因素对咽侧体活动的影响,应用放射化学法及免疫电泳测定了食物、卵巢发育、脑神经肽、保幼激素类似物对卵黄发生期成虫保幼激素生物合成及血淋巴中卵黄原蛋白含量的影响。结果表明咽侧体活性受上述各种因子的影响。咽侧体活性与卵黄原蛋白含量及卵母细胞生长密切相关,说明有反馈作用。食物的质与量影响着咽侧体活性的变化。低剂量外源保幼激素类似物处理成虫则可促使咽侧体活性的变化。脑分泌的神经肽(allatotropin)可活化咽侧体。这些结果表明雌瓢虫保幼激素的生产主要是受起源于脑的促咽侧体信号的调节作用。 相似文献
19.
以人胃癌细胞BGC-823为模型,研究了毛喉萜(forskolin)对胃癌细胞中蛋白激酶C活性及其亚类基因表达的作用,同时也观察了毛喉萜对癌基因c-jun及抑癌基因p53表达的影响.结果表明,2×10~(-5)mol/L毛喉萜处理BGC-823细胞72h,细胞质、膜和细胞核PKC活性下降,PKC亚类β,γ基因表达被抑制,癌基因c-jun的表达也明显降低,而抑癌基因p53表达升高,上述变化可能是毛喉萜抑制胃癌细胞增殖等生理效应的重要分子事件。 相似文献
20.
Transformation and pp60v-src autophosphorylation correlate with SHC-GRB2 complex formation in rat and chicken cells expressing host-range and kinase-active, transformation-defective alleles of v-src. 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《Molecular biology of the cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The biochemical properties of several pp60v-src substrates believed to participate in src-mediated transformation were examined in cells expressing a kinase-active, transformation-defective v-src allele (v-src-F172 delta/Y416F) and its parental allele, v-src-F172 delta, a host-range--dependent allele that transforms chicken cells to a fusiform morphology, but does not transform rat cells. Because pp60v-src-F172 delta is dependent on autophosphorylation for transforming ability, these alleles provide a unique opportunity to examine the role of pp60v-src autophosphorylation in regulating substrate interactions. Increased pp125FAK tyrosine phosphorylation and high levels of pp60v-src-associated phosphotidylinositol-3' kinase activity were detected specifically in chicken cells exhibiting round, refractile transformation but not in cells transformed to a fusiform morphology. Increased pp125FAK kinase activity, but not increased pp125FAK tyrosine-phosphorylation correlated with pp60v-src autophosphorylation and increased anchorage-independent growth. Thus, pp125FAK and PI3'K may participate in morphological transformation by v-src. Furthermore, association of phosphorylated SHC with the adapter GRB2 correlated with increased anchorage-independent growth (and autophosphorylation) in both rat and chicken cells independent of the morphological phenotype induced. Therefore, host-range dependence for transformation may be regulated through association of SHC with GRB2, thus implicating SHC as a crucial substrate for src-dependent transformation. 相似文献