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261.
Yalan Chen Zhangliu Du Zhe Weng Ke Sun Yuqin Zhang Qin Liu Yan Yang Yang Li Zhibo Wang Yu Luo Bo Gao Bin Chen Zezhen Pan Lukas Van Zwieten 《Global Change Biology》2023,29(18):5445-5459
To achieve long-term increases in soil organic carbon (SOC) storage, it is essential to understand the effects of carbon management strategies on SOC formation pathways, particularly through changes in microbial necromass carbon (MNC) and dissolved organic carbon (DOC). Using a 14-year field study, we demonstrate that both biochar and maize straw lifted the SOC ceiling, but through different pathways. Biochar, while raising SOC and DOC content, decreased substrate degradability by increasing carbon aromaticity. This resulted in suppressed microbial abundance and enzyme activity, which lowered soil respiration, weakened in vivo turnover and ex vivo modification for MNC production (i.e., low microbial carbon pump “efficacy”), and led to lower efficiency in decomposing MNC, ultimately resulting in the net accumulation of SOC and MNC. In contrast, straw incorporation increased the content and decreased the aromaticity of SOC and DOC. The enhanced SOC degradability and soil nutrient content, such as total nitrogen and total phosphorous, stimulated the microbial population and activity, thereby boosting soil respiration and enhancing microbial carbon pump “efficacy” for MNC production. The total C added to biochar and straw plots were estimated as 27.3–54.5 and 41.4 Mg C ha−1, respectively. Our results demonstrated that biochar was more efficient in lifting the SOC stock via exogenous stable carbon input and MNC stabilization, although the latter showed low “efficacy”. Meanwhile, straw incorporation significantly promoted net MNC accumulation but also stimulated SOC mineralization, resulting in a smaller increase in SOC content (by 50%) compared to biochar (by 53%–102%). The results address the decadal-scale effects of biochar and straw application on the formation of the stable organic carbon pool in soil, and understanding the causal mechanisms can allow field practices to maximize SOC content. 相似文献
262.
Biswas S Nyman JS Alvarez J Chakrabarti A Ayres A Sterling J Edwards J Rana T Johnson R Perrien DS Lonning S Shyr Y Matrisian LM Mundy GR 《PloS one》2011,6(11):e27090
Breast cancer often metastasizes to bone causing osteolytic bone resorption which releases active TGFβ. Because TGFβ favors progression of breast cancer metastasis to bone, we hypothesized that treatment using anti-TGFβ antibody may reduce tumor burden and rescue tumor-associated bone loss in metastatic breast cancer. In this study we have tested the efficacy of an anti-TGFβ antibody 1D11 preventing breast cancer bone metastasis. We have used two preclinical breast cancer bone metastasis models, in which either human breast cancer cells or murine mammary tumor cells were injected in host mice via left cardiac ventricle. Using several in vivo, in vitro and ex vivo assays, we have demonstrated that anti-TGFβ antibody treatment have significantly reduced tumor burden in the bone along with a statistically significant threefold reduction in osteolytic lesion number and tenfold reduction in osteolytic lesion area. A decrease in osteoclast numbers (p?=?0.027) in vivo and osteoclastogenesis ex vivo were also observed. Most importantly, in tumor-bearing mice, anti-TGFβ treatment resulted in a twofold increase in bone volume (p<0.01). In addition, treatment with anti-TGFβ antibody increased the mineral-to-collagen ratio in vivo, a reflection of improved tissue level properties. Moreover, anti-TGFβ antibody directly increased mineralized matrix formation in calverial osteoblast (p?=?0.005), suggesting a direct beneficial role of anti-TGFβ antibody treatment on osteoblasts. Data presented here demonstrate that anti-TGFβ treatment may offer a novel therapeutic option for tumor-induced bone disease and has the dual potential for simultaneously decreasing tumor burden and rescue bone loss in breast cancer to bone metastases. This approach of intervention has the potential to reduce skeletal related events (SREs) in breast cancer survivors. 相似文献
263.
264.
[目的] 从罗源湾红树林浅滩土壤中筛选出产脲酶真菌,研究其对镧La(Ⅲ)的最大耐受浓度,利用其吸附和产脲酶作用诱导矿化回收稀土离子La(Ⅲ),以期为稀土离子La(Ⅲ)的资源回收提供菌种资源和应用技术指导。[方法] 从罗源湾红树林浅滩土壤中分离、筛选、纯化出可产脲酶及耐La(Ⅲ)真菌,通过ITS rDNA基因序列分析对其进行鉴定;同时,利用XRD、SEM-Mapping及FT-IR分析探讨菌株回收La(Ⅲ)的机理。[结果] 经分离、纯化得到一株可产脲酶及耐受高浓度La(Ⅲ)的真菌FZU-07,鉴定为尖孢镰刀菌(Fusarium oxysporum),其具有较强诱导矿化回收La(Ⅲ)的能力,对La(Ⅲ)的最大耐受浓度为400 mg/L。菌株FZU-07单独对La(Ⅲ)吸附回收效率为46.19%;在诱导矿化条件下回收效率可提高到99.16%。FT-IR和SEM-Mapping分析表明,尖孢镰刀菌吸附La(Ⅲ)与菌丝体表面的氨基、羟基、羰基和磷酸基团相关;XRD和SEM-Mapping结果表明诱导矿化是通过该菌的产脲酶特性,使尿素分解产生碳酸,并与钙离子结合生成球霰石晶型的碳酸钙,La(Ⅲ)被捕获在球霰石晶格中,形成La(Ⅲ)和碳酸钙的混合固相,以共沉淀的形式被回收。[结论] 菌株FZU-07,是一株具有产脲酶特性的尖孢镰刀菌(Fusarium oxysporum),且具有较强的诱导矿化回收La(Ⅲ)能力。表明微生物诱导碳酸钙沉淀是一种可行且生态友好的回收稀土离子的方法。 相似文献
265.
266.
Yu Song Xin Yao Yunhong Tan Yi Gan Junbo Yang Richard T. Corlett 《Tree Genetics & Genomes》2017,13(6):120
Phoebe is an economically important genus from the family Lauraceae. It is widely distributed in tropical and subtropical Asia, but systematics of the genus is unclear, and currently there is no species-level phylogeny. Here, we determined the complete chloroplast genome sequences of two species with long-range PCR and next genome sequencing technologies, and identified mutation sites and highly variable regions. These highly variable sites were used to reconstruct the phylogeny. The plastomes of Phoebe sheareri and P. omeiensis were 152, 876, and 152, 855 bp, respectively. Comparative genomic analysis indicated that there are 222 mutation sites including 146 substitutions, 73 indels, and 3 microinversions in both plastomes. Fifty-six single-nucleotide changes were identified in gene-coding regions, and 45 microsatellite sites were found for use in species identification. Fourteen divergence hotspots of 38 variable regions were located. Phylogeny was reconstructed using a Bayesian and maximum likelihood approach for 12 Phoebe species and other five related Lauraceae based on 15 of the highly variable regions including accD-psaI, atpB-rbcL, ndhC-trnV, ndhF-rpl32, petA-psbJ, psaA, psbA-trnH, rbcL, rps8-rpl14, rps16-trnQ, rpl32-trnL, trnC-petN, trnL-trnF, trnS-trnG, and ycf1 indicated that variability in the chloroplast regions proposed as variable is enough to detect divergence events among 12 taxa of Phoebe, and that maybe also useful to help to elucidate further relationships among other taxa of the genus. 相似文献
267.
Diversity of endophytic bacteria within nodules of the Sphaerophysa salsula in different regions of Loess Plateau in China 总被引:1,自引:0,他引:1
Deng ZS Zhao LF Kong ZY Yang WQ Lindström K Wang ET Wei GH 《FEMS microbiology ecology》2011,76(3):463-475
A total of 115 endophytic bacteria were isolated from root nodules of the wild legume Sphaerophysa salsula grown in two ecological regions of Loess Plateau in China. The genetic diversity and phylogeny of the strains were revealed by restriction fragment length polymorphism and sequencing of 16S rRNA gene and enterobacterial repetitive intergenic consensus-PCR. Their symbiotic capacity was checked by nodulation tests and analysis of nifH gene sequence. This is the first systematic study on endophytic bacteria associated with S. salsula root nodules. Fifty of the strains found were symbiotic bacteria belonging to eight putative species in the genera Mesorhizobium, Rhizobium and Sinorhizobium, harboring similar nifH genes; Mesorhizobium gobiense was the main group and 65 strains were nonsymbiotic bacteria related to 17 species in the genera Paracoccus, Sphingomonas, Inquilinus, Pseudomonas, Serratia, Mycobacterium, Nocardia, Streptomyces, Paenibacillus, Brevibacillus, Staphylococcus, Lysinibacillus and Bacillus, which were universally coexistent with symbiotic bacteria in the nodules. Differing from other similar studies, the present study is the first time that symbiotic and nonsymbiotic bacteria have been simultaneously isolated from the same root nodules, offering the possibility to accurately reveal the correlation between these two kinds of bacteria. These results provide valuable information about the interactions among the symbiotic bacteria, nonsymbiotic bacteria and their habitats. 相似文献
268.
Evseeva Nina V. Tkachenko Oksana V. Denisova Alena Yu. Burygin Gennady L. Veselov Dmitry S. Matora Larisa Yu. Shchyogolev Sergei Yu. 《World journal of microbiology & biotechnology》2019,35(12):1-10
World Journal of Microbiology and Biotechnology - Derived from RNA, 5?-ribonucleotides, especially Inosine-5?-monophosphate (IMP) and guanosine-5?-monophosphate (GMP), can enhance... 相似文献
269.
Hongsheng Liu Long Yu George Simon Katherine Dean Ling Chen 《Carbohydrate polymers》2009,77(3):662-669
Corn starches with different amylose/amylopectin ratios (waxy 0/100, normal corn 23/77, Gelose 50 50/50, Gelose 80 80/20) were annealed at below their gelatinization temperatures in excess water. The effects of annealing on the gelatinization and microstructures of the starches were studied using DSC, XRD and a microscope equipped with both normal and polarized light. In addition, a high-pressure DSC pan was used to study the effects of high-temperature annealing on the multiphase transitions of starches with different water contents. The granular size of the starches increased after the annealing process, but the size variation rates were different, with higher amylopectin contents resulting in a higher diameter growth rates and final accretion ratios. DSC results showed that annealing increased the gelatinization enthalpy of the amylose-rich starches. The increased enthalpy was mainly attributed to endotherm G – there were no significant changes to endotherms M1, M2 or Z – indicating that annealing mainly affected the helical length of shorter or sub-optional amylopectins, in particular the amylopectin in amylose-rich starches. The XRD traces of all starches after annealing remained unchanged. 相似文献
270.