Fluorographic detection of tritiated glycopeptides and oligosaccharides separated on polyacrylamide gels: analysis of glycans from Dictyostelium discoideum glycoproteins

Previous workers have shown that oligosaccharides and glycopeptides can be separated by electrophoresis in buffers containing borate ions. However, normal fluorography of tritium-labeled structures cannot be performed because the glycans are soluble and can diffuse during equilibration with scintillants. This problem has been circumvented by equilibration of the gel with 2,5-diphenyloxazole (PPO) prior to electrophoresis. The presence of PPO in the gel during electrophoresis does not alter mobility of the glycopeptides and oligosaccharides. After electrophoresis, the gel is simply dried and fluorography performed. This allows sensitive and precise comparisons of labeled samples in parallel lanes of a slab gel and, since mobilities are highly reproducible, between different gels. The procedure is preparative in that after fluorography the gel bands can be quantitatively eluted for further study, without any apparent modification by the procedure. In this report, the procedure is illustrated by fractionation of both neutral and anionic glycopeptides produced by the cellular slime mold Dictyostelium discoideum.     

Wireworm populations in relation to crop production: I. A large-scale flotation method for extracting wireworms from soil samples and results from a survey of 600 fields

The wireworm survey in the Eastern Counties revealed many cases where the observed wireworm damage failed to correspond with the estimated field population. A possible explanation for this was the inaccuracy of counts made by picking wireworms out of the soil samples by hand. Tests showed that such methods recovered an extremely variable proportion of the wireworms in the- samples and, on the average, only two-fifths of the larvae were obtained. A modified form of the washing and flotation technique used by Salt & Hollick (1944) was introduced for large-scale work and is described. By this method, ten samples of soil (4 in. diam. and 6 in. deep) bulked together are examined at a time and can be dealt with at the rate of 13 samples per man per hour with an efficiency of 95-100% in the extraction of wireworms. The populations estimated on 600 fields sampled between December 1942 and May 1943 have thrown more light on the size and composition of the wireworm population in grass and arable fields. Inspection of the crop results on fields tested by the washing process showed a much closer relationship between wireworm population and wireworm damage than had been obtained by the hand-sorting method in the previous year.